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<h4>Endolysin Construct</h4> | <h4>Endolysin Construct</h4> | ||
<center><img src="https://static.igem.org/mediawiki/2017/c/c7/T--NYMU-Taipei--NS_endolysin_snapgene.png" style="width:45%;"></center> | <center><img src="https://static.igem.org/mediawiki/2017/c/c7/T--NYMU-Taipei--NS_endolysin_snapgene.png" style="width:45%;"></center> | ||
− | <p> We successfully constructed J23106-B0034-Endolysin-B0010-B0012. The endolysin in this part is from iGEM released part, BBa_K112806, which is endolysin from enterobacteria phage T4. Besides endolysin, in this composite part, we choose BBa_J23106 as a constitutive promoter, BBa_B0034 as ribosome binding site, BBa_B0010 and BBa_B0012 as double terminator, all of which are widely used parts in iGEM.</p> | + | <p> We successfully constructed <font class='mark_backbone'>J23106-B0034-Endolysin-B0010-B0012</font>. The endolysin in this part is from iGEM released part, BBa_K112806, which is endolysin from enterobacteria phage T4. Besides endolysin, in this composite part, we choose BBa_J23106 as a constitutive promoter, BBa_B0034 as ribosome binding site, BBa_B0010 and BBa_B0012 as double terminator, all of which are widely used parts in iGEM.</p> |
<div style="width:55%;margin:auto;"> | <div style="width:55%;margin:auto;"> | ||
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<h4></h4> | <h4></h4> | ||
<h4>Holin Construct</h4> | <h4>Holin Construct</h4> | ||
− | <p> We successfully constructed R0010-B0034-Holin-B0010-B0012. To control the precise suicide timing, we choose a lactose-induced promoter, BBa_R0010, to regulate this suicide mechanism. Besides holin and inducible promoter, in this composite part, we also choose BBa_B0034 as ribosome binding site, and BBa_B0010 and BBa_B0012 as double terminator.</p> | + | <p> We successfully constructed <font class='mark_backbone'>R0010-B0034-Holin-B0010-B0012</font>. To control the precise suicide timing, we choose a lactose-induced promoter, BBa_R0010, to regulate this suicide mechanism. Besides holin and inducible promoter, in this composite part, we also choose BBa_B0034 as ribosome binding site, and BBa_B0010 and BBa_B0012 as double terminator.</p> |
<div style="width:55%;margin:auto;"> | <div style="width:55%;margin:auto;"> | ||
<img src="https://static.igem.org/mediawiki/2017/9/9a/T--NYMU-Taipei--NS_holin_PCR.png" style="width:95%;"> | <img src="https://static.igem.org/mediawiki/2017/9/9a/T--NYMU-Taipei--NS_holin_PCR.png" style="width:95%;"> | ||
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<h4></h4> | <h4></h4> | ||
<h4>Endolysin-Holin Construct</h4> | <h4>Endolysin-Holin Construct</h4> | ||
− | <p> We successfully constructed R0010-B0034-Holin-B0010-B0012-J23106-B0034-Endolysin-B0010-B0012. We combined holin and endolysin for suicide mechanism. In this composite part, holin functions as an important regulation role.</p> | + | <p> We successfully constructed <font class='mark_backbone'>R0010-B0034-Holin-B0010-B0012-J23106-B0034-Endolysin-B0010-B0012</font>. We combined holin and endolysin for suicide mechanism. In this composite part, holin functions as an important regulation role.</p> |
<div style="width:55%;margin:auto;"> | <div style="width:55%;margin:auto;"> | ||
<img src="https://static.igem.org/mediawiki/parts/e/e2/T--NYMU-Taipei%E2%80%94Holin-Endolysin-REcheck.png" style="width:50%;"> | <img src="https://static.igem.org/mediawiki/parts/e/e2/T--NYMU-Taipei%E2%80%94Holin-Endolysin-REcheck.png" style="width:50%;"> | ||
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<h4></h4> | <h4></h4> | ||
<h4>Endolysin-Holin-NrtA Construct</h4> | <h4>Endolysin-Holin-NrtA Construct</h4> | ||
− | <p> We successfully constructed R0010-B0034-Holin-B0010-B0012-J23106-B0034-Endolysin-B0010-B0012-J23118-B0034-NrtA-B0015. This part combined holin, endolysin, and NrtA and had nitrate-capturing function and suicide function.</p> | + | <p> We successfully constructed <font class='mark_backbone'>R0010-B0034-Holin-B0010-B0012-J23106-B0034-Endolysin-B0010-B0012-J23118-B0034-NrtA-B0015</font>. This part combined holin, endolysin, and NrtA and had <font class='mark_backbone'>nitrate-capturing</font> function and <font class='mark_backbone'>suicide</font> function.</p> |
<div style="width:55%;margin:auto;"> | <div style="width:55%;margin:auto;"> | ||
<img src="https://static.igem.org/mediawiki/parts/b/b0/Holin-Endolysin-NrtA_REcheck.png" style="width:50%;"> | <img src="https://static.igem.org/mediawiki/parts/b/b0/Holin-Endolysin-NrtA_REcheck.png" style="width:50%;"> |
Revision as of 01:18, 29 October 2017