Difference between revisions of "Team:UNOTT/Experiments"

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       <h4 style="color: #ffffff; font-weight: bold; font-size: 30px;">STEP 1: CREATE guideRNA Plasmid </h4><center><img class="icons" src="https://static.igem.org/mediawiki/2017/1/11/T--UNOTT--guideRNA.png" style="width:300px;height:auto;"></center>
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<p>We first produced our gRNA plasmids in order to stop expression of targeted fluorescent proteins on our reporter plasmid. </p>
 
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Revision as of 00:22, 30 October 2017





EXPERIMENTS:

STEP 1: Create guideRNA Plasmid

STEP 2: CREATE pREPORTER

OBJECTIVE: PROMOTER LIBRARY

OBJECTIVE: RANDOM LIGATIONS

OBJECTIVE: FREEZE DRYING

OBJECTIVE: CRISPRi & gRNA EFFICIENCY