Difference between revisions of "Team:Lanzhou/Applied Design"

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<li><a href="#article_1">Crops Guardian</a></li>
 
<li><a href="#article_1">Crops Guardian</a></li>
<li><a href="#article_2">Dev2</a></li>
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<li><a href="#article_2">Bio-pesticides kit</a></li>
 
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<h1 class="mdc-typography--headline">Components</h1>
 
<h1 class="mdc-typography--headline">Components</h1>
<figure>
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<h2 class="mdc-typography--title">• Embedded device</h2>
<img src="https://static.igem.org/mediawiki/2017/9/97/Lanzhou_wiki_app_figure1.png" alt="">
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<figure class="small-picture">
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<img src="https://static.igem.org/mediawiki/2017/thumb/7/7b/Lanzhou_wiki_app_figure6.png/800px-Lanzhou_wiki_app_figure6.png" alt="" data-action="zoom">
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<img src="https://static.igem.org/mediawiki/2017/thumb/8/80/Lanzhou_wiki_app_figure7.png/526px-Lanzhou_wiki_app_figure7.png" alt="" data-action="zoom">
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<img src="https://static.igem.org/mediawiki/2017/thumb/1/12/Lanzhou_wiki_app_figure8.png/800px-Lanzhou_wiki_app_figure8.png" alt="" data-action="zoom">
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<img src="https://static.igem.org/mediawiki/2017/thumb/6/64/Lanzhou_wiki_app_figure9.png/800px-Lanzhou_wiki_app_figure9.png" alt="" data-action="zoom">
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<img src="https://static.igem.org/mediawiki/2017/a/a2/Lanzhou_wiki_app_figure10.png" alt="" data-action="zoom">
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<img src="https://static.igem.org/mediawiki/2017/thumb/8/8b/Lanzhou_wiki_app_figure11.png/800px-Lanzhou_wiki_app_figure11.png" alt="" data-action="zoom">
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<img src="https://static.igem.org/mediawiki/2017/thumb/c/c7/Lanzhou_wiki_app_figure12.png/800px-Lanzhou_wiki_app_figure12.png" alt="" data-action="zoom">
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<img src="https://static.igem.org/mediawiki/2017/thumb/2/23/Lanzhou_wiki_app_figure13.png/800px-Lanzhou_wiki_app_figure13.png" alt="" data-action="zoom">
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<img src="https://static.igem.org/mediawiki/2017/thumb/5/53/Lanzhou_wiki_app_figure14.png/800px-Lanzhou_wiki_app_figure14.png" alt="" data-action="zoom">
 
<figcaption>Figure 1. Sketch and model.</figcaption>
 
<figcaption>Figure 1. Sketch and model.</figcaption>
 
</figure>
 
</figure>
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<h2 class="mdc-typography--title">Real priduct</h2>
 
<figure>
 
<figure>
 
<img src="https://static.igem.org/mediawiki/2017/thumb/6/6c/Lanzhou_wiki_app_figure2.png/800px-Lanzhou_wiki_app_figure2.png" alt="">
 
<img src="https://static.igem.org/mediawiki/2017/thumb/6/6c/Lanzhou_wiki_app_figure2.png/800px-Lanzhou_wiki_app_figure2.png" alt="">
 
<figcaption>Figure 2. Real product.</figcaption>
 
<figcaption>Figure 2. Real product.</figcaption>
 
</figure>
 
</figure>
<p class="mdc-typography--boyd2">1. The ground part contains two joints for connecting water pipes, which could firmly link with PE hose. And the annular groove can prevent the soil from entering device.</p>
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<p class="mdc-typography--body2">1. The ground part contains two joints for connecting water pipes, which could firmly link with PE hose. And the annular groove can prevent the soil from entering device.</p>
<p class="mdc-typography--boyd2">2. A connector between the water pipe and the PE hose.</p>
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<p class="mdc-typography--body2">2. A connector between the water pipe and the PE hose.</p>
<p class="mdc-typography--boyd2">3. PE hose which is high strength, corrosion resistance, non-toxic and low cost.</p>
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<p class="mdc-typography--body2">3. PE hose which is high strength, corrosion resistance, non-toxic and low cost.</p>
<p class="mdc-typography--boyd2">4. The underground part consists of 10 centimeter device body and a sprinkler head.</p>
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<p class="mdc-typography--body2">4. The underground part consists of 10 centimeter device body and a sprinkler head.</p>
<p class="mdc-typography--boyd2">5. Spray part and spherical bottom. The spray part is made up of eight holes, which could supply water evenly. And the large spherical bottom can prevent the clogging or destruction during the insertion.</p>
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<p class="mdc-typography--body2">5. Spray part and spherical bottom. The spray part is made up of eight holes, which could supply water evenly. And the large spherical bottom can prevent the clogging or destruction during the insertion.</p>
<p class="mdc-typography--boyd2">6. Complete device</p>
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<p class="mdc-typography--body2">6. Complete device</p>
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<h2 class="mdc-typography--title">Mixing Device</h2>
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<figure><img src="https://static.igem.org/mediawiki/2017/thumb/3/3b/Lanzhou_wiki_app_figure15.png/800px-Lanzhou_wiki_app_figure15.png" alt="" data-action="zoom"></figure>
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<h1 class="mdc-typography--headline">Overall</h1>
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<p class="mdc-typography--body2">When the embedded device work with the mixing device , the overall effect diagram is like below.</p>
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<figure><img src="https://static.igem.org/mediawiki/2017/thumb/9/97/Lanzhou_wiki_app_figure1.png/800px-Lanzhou_wiki_app_figure1.png" alt="" data-action="zoom"></figure>
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<p class="mdc-typography--body2">
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This underground irrigation system not only reduces the losses of water, fertilizer and bio-pesticides but speeds up the weeding and killing pests efficiency by omitting permeation time.
 +
</p>
 +
<p class="mdc-typography--body2">
 +
Which is more economical and practical.
 +
</p>
  
 
<h1 class="mdc-typography--headline">Materials & Cost</h1>
 
<h1 class="mdc-typography--headline">Materials & Cost</h1>
<p class="mdc-typography--boyd2">We use 3D printing technology to produce this precise nozzle. The material is photosensitive resin -Somos GP Plus, which is a low viscosity photosensitive polymer, with the advantages of excellent waterproof, durability and hardness. It could be used for making solid, precision parts. Other part of the water pipe is PE texture, which has the characteristics of high strength, corrosion resistance, non-toxic and low-cost.</p>
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<p class="mdc-typography--body2">We use 3D printing technology to produce two part of devices. The material is photosensitive resin -Somos GP Plus, which is a low viscosity photosensitive polymer, with the advantages of excellent waterproof, durability and hardness. It could be used for making solid, precision parts. Other part of the water pipe is PE texture, which has the characteristics of high strength, corrosion resistance, non-toxic and low-cost.</p>
<p class="mdc-typography--boyd2">3D printing is not suitable for the volume production. If our device use the mold to produce in batches, the unit price will drop to a very low level.</p>
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<p class="mdc-typography--body2">3D printing is not suitable for the volume production. If our device use the mold to produce in batches, the unit price will drop to a very low level.</p>
 
<figure>
 
<figure>
 
<img src="https://static.igem.org/mediawiki/2017/0/0c/Lanzhou_wiki_app_figure3.png" alt="">
 
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</article>
 
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<article id="article_2" class="page__article"></article>
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<article id="article_2" class="page__article">
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<h1 class="mdc-typography--headline">Overview</h1>
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<p class="mdc-typography--body2">
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We designed a kit model for our Bio-pesticides application in future. The kit allows users to  to cultivate engineered bacteria for dsRNA production by themselves. It reduces the cost greatly and make the bio-pesticides have ability to compete with the chemical pesticides in market.
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</p>
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<p class="mdc-typography--body2">
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The device can ensure aseptic operation and can be reused.
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</p>
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<h1 class="mdc-typography--headline">Components</h1>
 +
<h2 class="mdc-typography--title">Appearance</h2>
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<figure>
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<img src="https://static.igem.org/mediawiki/2017/1/17/Lanzhou_wiki_app_figure16.png" alt="">
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</figure>
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<h2 class="mdc-typography--title">Inside</h2>
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<figure>
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<img src="https://static.igem.org/mediawiki/2017/thumb/4/42/Lanzhou_wiki_app_figure17.png/800px-Lanzhou_wiki_app_figure17.png" alt="" data-action="zoom">
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</figure>
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<h1 class="mdc-typography--headline">Usage</h1>
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<p class="mdc-typography--body2">
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1. Open the culture bottle, pour the powder into the medium, pour in the boiled water, change the rubber plug with aluminum shell, shake it and make the culture .
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</p>
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<p class="mdc-typography--body2">
 +
2. Using a clean needle to insert into the glycerol bacteria bottle to extract glycerol bacteria, when the culture fluid cold, taking bacteria liquid into the culture bottle and shaking the culture solution
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</p>
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<p class="mdc-typography--body2">
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3. Culture in room temperature and after 24 hours,pour the lytic phage powder into the culture bottle to lysed E. coli. Insert the culture bottle at the interface of the watering pipe, then dsRNA can be poured into the field.
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</p>
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<p class="mdc-typography--body2">
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4. After using, then wash the bacteria solution with boiled water to sterilize it for reuse.
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</p>
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<h1 class="mdc-typography--headline">Conclusion</h1>
 +
<p class="mdc-typography--body2">
 +
We hope that when we finished our future work and create a mature Bio-pesticide product, our kit could be put into production and really help the peasants all over the world to fight with weeds and pests.
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</p>
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Revision as of 14:44, 30 October 2017

Lanzhou

Lanzhou2017

Overview

Can you imagine an application which could water and fertilize crops accurately under the soil ? Our team’s watering device dose!

Traditional irrigation technology depends on water permeation from soil surface to the plant roots, this process will lose much water and fertilizer, besides lead soil hardening.

And our device, an improvement of micro-irrigation, perfectly solve this problem. It aims at target irrigation, supplying water and fertilizer for plant roots directly and precisely, with the advantages of multifunctional, more energy-saving and higher water transport efficiency.

Weeds and pests are the most important damages to agronomy system, which resulted in agriculture economic losses of more than $20.4 billion in 1972. Merely taking the United States as an example, the damage is $8.4 billion a year, while pests account for 40% and weeds account for 60%. In our country, China, the crop yield loss is almost fifty million kilogram per year.

Note : Micro-irrigation system is the most advanced irrigation system right now, through transporting the pressure water to the fields, it can irrigate the soil near the root of the plant by only a small flow of water permeation. The main superiority of micro-irrigation are energy saving, evenly irrigation and the strong adaptability to soil and topography, which can increase crop production and field salt tolerance.

Principle

Our device can not only provide nutrients and water for crops, but also take the herbicides to weed roots and kill weeds. So we call it“Crops Guardian”.

During the design, we aimed at one of the most widely distributed weeds family, Gramineae , whose roots locate about 10cm under the ground. According to this character, we determined our device parameters available for it.

Components

• Embedded device

Figure 1. Sketch and model.

Real priduct

Figure 2. Real product.

1. The ground part contains two joints for connecting water pipes, which could firmly link with PE hose. And the annular groove can prevent the soil from entering device.

2. A connector between the water pipe and the PE hose.

3. PE hose which is high strength, corrosion resistance, non-toxic and low cost.

4. The underground part consists of 10 centimeter device body and a sprinkler head.

5. Spray part and spherical bottom. The spray part is made up of eight holes, which could supply water evenly. And the large spherical bottom can prevent the clogging or destruction during the insertion.

6. Complete device

Mixing Device

Overall

When the embedded device work with the mixing device , the overall effect diagram is like below.

This underground irrigation system not only reduces the losses of water, fertilizer and bio-pesticides but speeds up the weeding and killing pests efficiency by omitting permeation time.

Which is more economical and practical.

Materials & Cost

We use 3D printing technology to produce two part of devices. The material is photosensitive resin -Somos GP Plus, which is a low viscosity photosensitive polymer, with the advantages of excellent waterproof, durability and hardness. It could be used for making solid, precision parts. Other part of the water pipe is PE texture, which has the characteristics of high strength, corrosion resistance, non-toxic and low-cost.

3D printing is not suitable for the volume production. If our device use the mold to produce in batches, the unit price will drop to a very low level.

Figure 3. A working 3D printer: Union Tech-RS Prp 600.

Usage

1st Connect the composite device between the ends of two pipes.

2nd Punch the pipe wall with a puncher.

3rd Then link the device to the water pipe through the connecting head.

4th Insert the head into the soil, open the tap water to irrigate.

5th Add pesticides and fertilizers to the Mixing device.

Figure 4. Installation complete.
The crops guardian will start working

Performance & feedback

We put the device in the soil to test it performance. And we got conclusion that the effective watering radius of our device can up to 25cm, which is equivalent to the moisture that 20 - 30 wheat individuals are needed. Moreover, the water saving effect of this device is far more than traditional irrigation and sprinkler irrigation system.

Meanwhile, for gaining real user experience, we found some voluntary farmers to let them use our product for a while. We gave them instructions and helped them with installation. After a week, we got the feedback, which strengthened our confidence to perfect it and finally bring it to the market in the near future.

Figure 5. Feed from farmers.

The positive side:

Most of them think the watering device is novel and easy to use, which is very convenient. In the northwestern China, areas are always arid, so they praised that the water-saving effects of our system are much better than any other irrigation methods. Also, they are touched by the integration of watering, fertilization and weeding of our device.

Areas for improvement:

Of course, as a preliminary design, our device also has some shortcomings. Like, for some deeper roots plants, the watering effect is not as good as to the shallow ones. And may be one field need several different size pipes to work together.

Conclusion

On the whole, they think this is a great invention. And they hope that our devices can be put into production in the near future and bring convenience to other farmers.

Overview

We designed a kit model for our Bio-pesticides application in future. The kit allows users to to cultivate engineered bacteria for dsRNA production by themselves. It reduces the cost greatly and make the bio-pesticides have ability to compete with the chemical pesticides in market.

The device can ensure aseptic operation and can be reused.

Components

Appearance

Inside

Usage

1. Open the culture bottle, pour the powder into the medium, pour in the boiled water, change the rubber plug with aluminum shell, shake it and make the culture .

2. Using a clean needle to insert into the glycerol bacteria bottle to extract glycerol bacteria, when the culture fluid cold, taking bacteria liquid into the culture bottle and shaking the culture solution

3. Culture in room temperature and after 24 hours,pour the lytic phage powder into the culture bottle to lysed E. coli. Insert the culture bottle at the interface of the watering pipe, then dsRNA can be poured into the field.

4. After using, then wash the bacteria solution with boiled water to sterilize it for reuse.

Conclusion

We hope that when we finished our future work and create a mature Bio-pesticide product, our kit could be put into production and really help the peasants all over the world to fight with weeds and pests.