Team:Lanzhou/Parts

Lanzhou

Lanzhou2017
Basic part
Name Type Description Designer Length
BBa_K2377000 DNA Intron loop with Aphids specificity Zhicheng Lin 194
BBa_K2377001 DNA Intron loop with Arabidopsis specificity He Jindian, Zhicheng Lin 106
BBa_K2377002 Coding Celluse coding sequence Zhicheng Lin 1500
BBa_K2377003 Coding Pectinase coding sequence Zhicheng Lin 1047
BBa_K2377010 DNA EcrL1 , the sense fragment of Ecr gene He Jindian, Zhang Shuting 523
BBa_K2377011 DNA EcrL2 , the anti-sense fragment of Ecr gene He Jindian, Zhang Shuting, Zhicheng Lin 523
BBa_K2377012 DNA Trxz sense fragment He Jindian 552
BBa_K2377013 DNA Trxz anti-sense fragment He Jindian 552
BBa_K2377014 Regulatory zarp, zinc two-component relulatory system Zhou Tuoyu 201
BBa_K2377015 DNA loop120 Zhicheng Lin 121
Composite part
Name Type Description Designer Length
BBa_K2377004 DNA Thioredoxin Z with two reverse T7 promoters and double terminators to generate dsRNA Zhicheng Lin, Ren Yi, He jindian, Huang Zonghui 904
BBa_K2377005 DNA TrxzL, A developed element to generate trxz hpRNA with a normal loop Zhicheng Lin, Ren Yi, He jindian, Huang Zonghui 1401
BBa_K2377006 DNA Replace intron loop with loop120( selected form A-1 chloramphenicol O-acetyltransferase) in BBa_K2377005 Zhicheng Lin, Ren Yi, He jindian, Huang Zonghui 1386
BBa_K2377007 DNA EcrL, one element to genarete Ecr hpRNA Zhicheng Lin 1358
BBa_K2377008 DNA ecrN Zhicheng Lin 1431
BBa_K2377009 DNA zrap ribB Zhou Tuoyu 1029
Plasmid backbone
Name Type Description Designer Length
BBa_K2377016 Plasmid backbone pDP: plasmid backbone for dsRNA production Zhicheng Lin 2349

Main part

BBa_K2377004

BBa_K2377009 is the initial part of our project. It is made up of Thioredoxin z (TRXz) gene fragment with two reverse T7 promoters and double terminators. It have been shown that silencing of TRX z result in a PEP-deficient phenotype which renders the plants incapable to grow autotrophically with their leaves being yellow.

BBa_K2377005

Traditional way to generate RNAi gene silencer using two reverse T7 promotors has a relative low efficiency due to the interference of two RNA polymerases during transcription process. To address this problem, we designed hpRNA with a loop between sense and antisense form of target sequence. It can be generated using only one promotor.

At first, we used loop120 (a 120bp sequence selected from Chl resistant gene.) as our loop. Afterwards, we saw from papers that the intron sequence in hpRNA can be spliced by spliceosome in plant. This process raises the efficiency of RNAi in plant by an unknown mechanism. Therefore, intron could be a better option of loop.

BBa_K2377007

Acyrthosiphon pisum ecdysone receptor (Ecr) is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003383721.1). ECR silencing can effectively reduce the expression of vital genes for Aphids development, thereby reducing the survival rate of insects and reproduction rate. This part is the sense arm of EcrL with loop120.

BBa_K2377002/BBa_K2377003

We design this two part for helping our dsRNA invade plant. Traces of pectinase and cellulase can cause