Description

Tell us about your project, describe what moves you and why this is something important for your team.

Key. coli

• The first biological password that changes over time! We are looking into transforming bacteria with a unique array of existing iGEM systems to produce a unique signal of secondary metabolites, initially using fluorescence as a proof of concept. Eventually, we will use the system to produce a unique and random configuration of secondary metabolites, as our "key". In order to produce this randomness, alteration of the activity or presence of promoters associated with these metabolites will be applied using one of a few methods currently being considered by the team.
• This key will be used to open locked mechanism such as safes and secure doors. We see a system where the measurement of key engineered metabolites such as volatiles will give a distinct mass spectrum. A juxtaposition of a detection technique such as gas chromotography-mass spectrometry and data comparison software will compare the secondary metabolites of the "key" bacteria to the "reference/lock" from which it was taken. If the spectra of both colonies exceeds a threshold of similarity the locked object will become unlocked.
• For the key to be practical it would need to be portable, this is where the design of our key transport device comes in. We are currently looking into a system of a similar design to a chemostat where a continual supply of medium will allow maintenance of a culture. Other options we are looking into include the use of freeze-dried cells or microfluidics.
• After a certain amount of time, our Key will have to be renewed from the Lock colony, and when this occurs the configuration of the key is shuffled once again to ensure the key and lock are changing.

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