Difference between revisions of "Team:UNOTT/Experiments"

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<li> The <i> E. coli </i> cells must be able to be activated after inactivation to allow the fluorescent genes to be expressed to give the key its unique fluorescent code which will allow access to the appliance.</li>  
 
<li> The <i> E. coli </i> cells must be able to be activated after inactivation to allow the fluorescent genes to be expressed to give the key its unique fluorescent code which will allow access to the appliance.</li>  
 
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To accomplish this, we chose to freeze dry the cells within the key. This involved.
 
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Revision as of 22:07, 30 October 2017





EXPERIMENTS:

STEP 1: Create guideRNA Plasmid

STEP 2: Create Reporter Plasmid

STEP 3: Promoter Library

STEP 4: Random Ligations

STEP 5: Freeze Drying & Revival

STEP 6: CRISPRi & gRNA Efficiency