Difference between revisions of "Team:UNOTT/Experiments"
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<p>For <i>Key. coli</i> to work as intended and not deteriorate we need need to things to occur: | <p>For <i>Key. coli</i> to work as intended and not deteriorate we need need to things to occur: | ||
| − | <ol> | + | <ol style="font-color:#ffffff;"> |
<li style="font-color:#ffffff;">The <i> E. coli </i> cells must be kept inactive so that nutrients is not depleted causing the transformed cells to die </li> | <li style="font-color:#ffffff;">The <i> E. coli </i> cells must be kept inactive so that nutrients is not depleted causing the transformed cells to die </li> | ||
<li style="font-color:#ffffff;"> The <i> E. coli </i> cells must be able to be activated after inactivation to allow the fluorescent genes to be expressed to give the key its unique fluorescent code which will allow access to the appliance.</li> | <li style="font-color:#ffffff;"> The <i> E. coli </i> cells must be able to be activated after inactivation to allow the fluorescent genes to be expressed to give the key its unique fluorescent code which will allow access to the appliance.</li> | ||
Revision as of 22:28, 30 October 2017
EXPERIMENTS:
STEP 1: Create guideRNA Plasmid
STEP 2: Create Reporter Plasmid
STEP 3: Promoter Library
STEP 4: Random Ligations
STEP 5: Freeze Drying & Revival
