Difference between revisions of "Team:UNOTT/Experiments"

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       <div id="clear6" style="display: none;">
 
       <div id="clear6" style="display: none;">
 
       <p>For <i>Key. coli</i> to work as intended and not deteriorate we need need to things to occur:  
 
       <p>For <i>Key. coli</i> to work as intended and not deteriorate we need need to things to occur:  
 +
<br>
 
<ol>
 
<ol>
 
<li><span>The <i> E. coli </i> cells must be kept inactive so that nutrients is not depleted causing the transformed cells to die </span></li>
 
<li><span>The <i> E. coli </i> cells must be kept inactive so that nutrients is not depleted causing the transformed cells to die </span></li>

Revision as of 22:33, 30 October 2017





EXPERIMENTS:

STEP 1: Create guideRNA Plasmid

STEP 2: Create Reporter Plasmid

STEP 3: Promoter Library

STEP 4: Random Ligations

STEP 5: Freeze Drying & Revival

STEP 6: CRISPRi & gRNA Efficiency