Difference between revisions of "Team:UIOWA"

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<h1> Welcome to iGEM 2017! </h1>
 
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
 
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<h5>Before you start: </h5>
 
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<p> Please read the following pages:</p>
 
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<ul>
 
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
 
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
 
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
 
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<div style="text-align:center; margin-left: 250px;">
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<h1 align="center"><font face="Arial">System Level Design</font></h1>
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<h5> Styling your wiki </h5>
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<table id="t01" style = "float: right;">
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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    <th>Input</th>
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    <th>Output</th>  
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    <td id="t01">Absent</td>
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    <td>Off</td>
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    <td>Present</td>
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    <td id="t01">On</td>
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<td><strong>System:</strong> 3HP Biosensor in <i>Bacillus Subtilis</i> </td>
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<td><strong>Input:</strong> 3HP</td>
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<td><strong>Output:</strong> Luminescence </td>
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<h5> Wiki template information </h5>
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<h1 align="center">Device Level Design</h1>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<img src="https://static.igem.org/mediawiki/2017/e/e8/T--UIOWA--3HP_2.jpg" width=1000px style="padding-top: 2px;">
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<h2 align=center>Refining a 3-Hydroxypropionic Acid Biosensor</h2>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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<a href="https://2017.igem.org/Special:Upload">
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<h2 align=center>Brief description of our motivation and goals</h2>
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UPLOAD FILES
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<p> Many industrial manufacturing processes revolve around the molecule 3-hydroxypropionic acid (3-HP). This organic molecule can be used in a variety of industrial products, from biofuels to plastic production. As a biologically produced chemical, 3-HP is biodegradable and thus a better substitute for inorganic plastics for environmental reasons. As 3-HP-derived plastics degrade, they release carbon dioxide, but do not persist for thousands of years in landfills (as PET plastics do). While much of the field is focusing on maximizing the production of this important molecule, a much smaller subset is focused on finding ways to sense and measure its production in an effort to aid industrial manufacturing systems. In a recent study, genes from the bacteria <i>Pseudomonas putida</i> were incorporated into <i>Escherichia coli</i> and <i>Cupriavidus necator</i> and demonstrated that repurposed regulatory proteins from <i>P. putida</i> could be used as a biosensor for 3-HPA (Hanko et al. 2017). A separate study identified similar 3-HP responsive genes in <i>Pseudomonas denitrificans</i> (Zhou et al. 2015).
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<p> Our summer iGEM research project seeks to use the same 3-HP responsive genes found in <i>P. putida</i> and <i>P. denitrificans</i> as switches for a reporter. This reporter will ‘program’ our bacteria to express a unique color in response to the presence of 3-HP. Additionally, we are seeking to add this system to <i>Bacillus subtilis</i>, a hardy bacterium that has great potential as a 3-HP producer for industrial processes and metabolic engineering experiments. <i>B. subtilis</i> can withstand high concentrations of 3-HP without triggering ‘toxicity’ pathways. By modifying naturally occurring proteins with 3-HP sensitive genes in new organisms, we can tune our biosensor to be used for different purposes in research and industry. This collection of 3-HP responsive biological tools can also be used by future iGEM teams around the world, as we will package our system in biological packets known as Biobricks. Similar to Lego pieces snapping together, Biobricks can be easily incorporated into bacteria by other iGEM teams to build larger projects with an amalgam of these biological building blocks.
 
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Latest revision as of 12:14, 1 November 2017


System Level Design

Input Output
Absent Off
Present On
System: 3HP Biosensor in Bacillus Subtilis
Input: 3HP
Output: Luminescence

Device Level Design

Refining a 3-Hydroxypropionic Acid Biosensor

Brief description of our motivation and goals

Many industrial manufacturing processes revolve around the molecule 3-hydroxypropionic acid (3-HP). This organic molecule can be used in a variety of industrial products, from biofuels to plastic production. As a biologically produced chemical, 3-HP is biodegradable and thus a better substitute for inorganic plastics for environmental reasons. As 3-HP-derived plastics degrade, they release carbon dioxide, but do not persist for thousands of years in landfills (as PET plastics do). While much of the field is focusing on maximizing the production of this important molecule, a much smaller subset is focused on finding ways to sense and measure its production in an effort to aid industrial manufacturing systems. In a recent study, genes from the bacteria Pseudomonas putida were incorporated into Escherichia coli and Cupriavidus necator and demonstrated that repurposed regulatory proteins from P. putida could be used as a biosensor for 3-HPA (Hanko et al. 2017). A separate study identified similar 3-HP responsive genes in Pseudomonas denitrificans (Zhou et al. 2015).

Our summer iGEM research project seeks to use the same 3-HP responsive genes found in P. putida and P. denitrificans as switches for a reporter. This reporter will ‘program’ our bacteria to express a unique color in response to the presence of 3-HP. Additionally, we are seeking to add this system to Bacillus subtilis, a hardy bacterium that has great potential as a 3-HP producer for industrial processes and metabolic engineering experiments. B. subtilis can withstand high concentrations of 3-HP without triggering ‘toxicity’ pathways. By modifying naturally occurring proteins with 3-HP sensitive genes in new organisms, we can tune our biosensor to be used for different purposes in research and industry. This collection of 3-HP responsive biological tools can also be used by future iGEM teams around the world, as we will package our system in biological packets known as Biobricks. Similar to Lego pieces snapping together, Biobricks can be easily incorporated into bacteria by other iGEM teams to build larger projects with an amalgam of these biological building blocks.