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| | {{BIT-China}} | | {{BIT-China}} |
| | <html> | | <html> |
| | + | <style> |
| | + | .my-banner2 { |
| | + | width: 100%; text-align: center; |
| | + | /*background-image: url(https://static.igem.org/mediawiki/2017/f/ff/T--BIT-China--2017home-background.jpg);*/ |
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| | + | } |
| | + | .my-banner2 img { |
| | + | width: 75%; |
| | | | |
| − | <div class="column full_size" > | + | } |
| − | [[File:Bit-china-2017logo.svg]]
| + | </style> |
| − | </div> | + | <body> |
| | + | <div class="my-banner"> |
| | + | <img src="https://static.igem.org/mediawiki/2017/6/67/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171031093107.jpg" alt=""> |
| | + | </div> |
| | + | |
| | + | <section class="content_container" id="mytop"> |
| | + | <section class="cd-section"> |
| | + | <h2 class="title-h2">ABSTRACT</h2> |
| | + | <p class="my-content-p"> |
| | + | In a parallel galaxy, there were two brothers named T1R2 and T1R3, they lived in a galaxy that was under the threat of sweet monsters who wanted to rule the galaxy. Though the brothers were born with the ability to eat these monsters, they were too weak to fight with so many enemies. To be stronger, they asked the battle creatures who lived in planet JB486 for help and sent their genes to a battle king SC84117 in the meantime. Driven by a mysterious force from the depths of the universe, the battle king, after adopting the gene <i>T1R2/T1R3</i>, lost its genes <i>ste2</i>, <i>sst2</i> and <i>far1</i>. After integration with <i>T1R2/T1R3</i> perfectly, something magical happened, a spaceship with a red ruler appeared. The battle king transformed into this spaceship which can eat sweet monsters, known as the Sugar Hunter. Since then, he travels all around the galaxy to fight sweet monsters and use the red ruler to measure them, the galaxy stays peaceful ever after.</p> |
| | + | </section> |
| | + | </section> |
| | + | <div class="my-banner2" id="top"> |
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| | + | <img src="https://static.igem.org/mediawiki/2017/a/a0/2222.png" alt=""> |
| | + | <img src="https://static.igem.org/mediawiki/2017/7/74/T--BIT-China--2017homepic2.jpg" alt=""> |
| | + | <img src="https://static.igem.org/mediawiki/2017/d/d5/2223.png" alt=""> |
| | + | <img src="https://static.igem.org/mediawiki/2017/2/24/T--BIT-China--2017homepic4.jpg" alt=""> |
| | | | |
| | + | </div> |
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| | | | |
| | + | <section class="content_container_img" id="mytop"> |
| | + | <div class="my-home-box"> |
| | + | |
| | + | <a href="https://2017.igem.org/Team:BIT-China/Project/Description"> |
| | + | <img src="https://static.igem.org/mediawiki/2017/d/d9/Bit-china-2017project.jpg" alt=""> |
| | + | </a> |
| | + | <a href="https://2017.igem.org/Team:BIT-China/Model"> |
| | + | <img src="https://static.igem.org/mediawiki/2017/a/a5/Bit-china-2017modeling.jpg" alt=""> |
| | + | </a> |
| | + | <a href="https://2017.igem.org/Team:BIT-China/Material"> |
| | + | <img src="https://static.igem.org/mediawiki/2017/e/ec/T--BIT-China--2017laboratory.jpg" alt=""> |
| | + | </a> |
| | + | </div> |
| | + | <div class="my-home-box"> |
| | + | <a href="https://2017.igem.org/Team:BIT-China/Parts"> |
| | + | <img src="https://static.igem.org/mediawiki/2017/f/fb/Bit-china-2017achievements.jpg" alt=""> |
| | + | </a> |
| | + | <a href="https://2017.igem.org/Team:BIT-China/HPSilver"> |
| | + | <img src="https://static.igem.org/mediawiki/2017/0/0a/Bit-china-2017human-practice.jpg" alt=""> |
| | + | </a> |
| | + | <a href="https://2017.igem.org/Team:BIT-China/Member"> |
| | + | <img src="https://static.igem.org/mediawiki/2017/d/d8/Bit-china-2017team.jpg" alt=""> |
| | + | </a> |
| | + | </div> |
| | + | </section> |
| | + | </body> |
| | + | </html> |
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| − | <div class="column full_size" >
| + | {{BIT-China/footer}} |
| − | <h1> Sweet Sensing Yeast </h1>
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| − | <p></p>
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| − | <h2> Description </h2>
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| − | <p>Low-caloric artificial sweeteners (LAS) and Low-caloric natural sweeteners (LNS) are among the most widely used food additives worldwide, regularly consumed by lean and obese individuals alike. With the improvement of people's living standard，desired sweeteners are expected far more eagerly due to the growing living level and the developing science technology. The goal of this work is to introduce the human sweet receptor T1R2-T1R3 system to create a practical application of a sweetener sensor using an engineered yeast strain. Initially，we replacing the endogenous yeast G-protein Ste2 with the human sweet receptor T1R2-T1R3, additionally, we deleted some genes to further improve the sensitivity of the T1R2-T1R3 reporter signal. And we also introduce a RFP expression cassette to characterized the relative sweetness degree by RFP fluorescence intensity. Our work will assist the development of a fluorescence-based - sweetening agent sensing system using engineered yeast.</p>
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| − | <p>GPCRs constitute the largest family of membrane proteins and are involved in the majority of signal transduction. The common architecture of GPCRs has a seven transmembrane domain structure (<i>Fredriksson et al.</i>, 2003; <i>Takeda et al.</i>, 2002) that responds to a remarkable range of stimuli, including neurotransmitters, hormones, and ions (<i>Lefkowitz and Shenoy</i>, 2005). G proteins are binary switches that are ‘off’ when bound to guanosine diphosphate (GDP) and ‘on’ when bound to guanosine triphosphate (GTP). The P-loop is responsible for the correct positioning of the triphosphate moiety of the bound nucleotide (<i>M. Saraste</i>,1990). Activation of the G protein stabilises the effector binding site via an interaction with a conserved threonine residue in switch I and a glycine residue in the switch II region, which form hydrogen bonds with the γ-phosphate of GTP in combination with a Mg<sup>2+</sup> cofactor. This functionality is conferred by an ∼20 kDa G domain that is conserved across all G proteins .
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| − | <div class="clear"></div>
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| − | <h5>Before you start: </h5>
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| − | <p> Please read the following pages:</p>
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| − | <ul>
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| − | <li> <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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| − | <li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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| − | <li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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| − | </ul>
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| − | </div>
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| − | <div class="column half_size" >
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| − | <div class="highlight">
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| − | <h5> Styling your wiki </h5>
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| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| − | </div>
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| − | </div>
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| − | <div class="column full_size" >
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| − | <h5> Wiki template information </h5>
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| − | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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| − | <h5> Editing your wiki </h5>
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| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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| − | <p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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| − | </div>
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| − | <div class="column half_size" >
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| − | <h5>Tips</h5>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <ul>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li>
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| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | </ul>
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| − | </div>
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| − | <div class="column half_size" >
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| − | <h5>Inspiration</h5>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <ul>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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| − | </ul>
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| − | </div>
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| − | <h5> Uploading pictures and files </h5>
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| − | <p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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| − | When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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| − | <a href="https://2017.igem.org/Special:Upload">
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| − | UPLOAD FILES
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| − | </a>
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| − | </p>
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| − | </div>
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| − | </html>
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ABSTRACT
In a parallel galaxy, there were two brothers named T1R2 and T1R3, they lived in a galaxy that was under the threat of sweet monsters who wanted to rule the galaxy. Though the brothers were born with the ability to eat these monsters, they were too weak to fight with so many enemies. To be stronger, they asked the battle creatures who lived in planet JB486 for help and sent their genes to a battle king SC84117 in the meantime. Driven by a mysterious force from the depths of the universe, the battle king, after adopting the gene T1R2/T1R3, lost its genes ste2, sst2 and far1. After integration with T1R2/T1R3 perfectly, something magical happened, a spaceship with a red ruler appeared. The battle king transformed into this spaceship which can eat sweet monsters, known as the Sugar Hunter. Since then, he travels all around the galaxy to fight sweet monsters and use the red ruler to measure them, the galaxy stays peaceful ever after.
