Team:BIT-China/Parts

BIT-CHINA

Biobrick	Name	Description	Length
Receptor expression
BBa_2368003	T1R2	Human Sweet-receptor	2520
BBa_2368004	T1R3	Human Sweet-receptor	1989
BBa_2368007	P_{Gal 1/10}-T1R2-CYC1t	Completed expression of the human Sweet-receptor	3449
BBa_2368008	P_{Gal 1/10}-T1R3-ADH1	Completed expression of the human Sweet-receptor	2814
BBa_2368023	MYC+T1R2 overlap	Marker genes of the Sweet-receptor	349
BBa_2368009	HIS+T1R2 overlap	Marker genes of the Sweet-receptor	50
BBa_2368024	HIS+T1R3 overlap	Marker genes of the Sweet-receptor	187
BBa_2368011	FLAG+T1R3 overlap	Marker genes of the Sweet-receptor	50
BBa_2368012	YCP+T1R2 overlap	Marker genes of the Sweet-receptor	740
BBa_2368013	YCP+T1R3 overlap	Marker genes of the Sweet-receptor	740
BBa_2368014	BCP+T1R2 overlap	Marker genes of the Sweet-receptor	710
BBa_2368015	GFP+T1R2 overlap	Marker genes of the Sweet-receptor	699
BBa_2368020	BCP+T1R3 overlap	Marker genes of the Sweet-receptor	710
Host engineering
BBa_2368017	500bp Homologous arm＋His	Knock out the sst2 gene	2050
BBa_2368018	500bp Homologous arm＋Ura	Knock out the far1 gene	2050
BBa_2368016	500bp Homologous arm＋Trp	Knock out the ste2 gene	2071
BBa_2368021	Modified Gα Schizosaccharomyces pombe	Signal transduction	1421
BBa_2368022	Modified Gα Caenorhabditis elegans	Signal transduction	1418
BBa_2368028	Gα	Signal transduction	1421
Detection circuit
BBa_2368005	P_fus	Induced promoter	438
BBa_2368026	P_fus(4 STE12 binding sites)	Engineered promoter	223
BBa_2368027	P_fus(6 STE12 binding sites)	Engineered promoter	249
BBa_2368006	P_fus+mRFP+CYC1t	The reporter	1375
BBa_2368002	CYC1t	A terminator in the yeast	231
BBa_2368029	P_fus(2 STE12 binding sites)	Engineered promoter	213

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