Difference between revisions of "Team:BIT-China/Parts"
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<img src="https://static.igem.org/mediawiki/2017/f/fb/Bit-china-2017achievements.jpg" alt=""> | <img src="https://static.igem.org/mediawiki/2017/f/fb/Bit-china-2017achievements.jpg" alt=""> | ||
</div> | </div> | ||
| − | <div style="box-shadow: 0 0 0 transparent; margin-bottom: 100px; | + | <div style="box-shadow: 0 0 0 transparent; margin-bottom: 100px;" class="my-banner" id="top"> |
| − | <img style="width: | + | <img style="width: 70% !important; margin-left: 15% !important; margin-top: 100px !important" src="https://static.igem.org/mediawiki/2017/e/e6/T-BIT-China-2017partniu.jpeg" alt=""> |
</div> | </div> | ||
<section class="content_container" id="mytop"> | <section class="content_container" id="mytop"> | ||
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</thead> | </thead> | ||
| − | <tbody> | + | <tbody style="background-color: rgba(255,180,0,0.1);"> |
<tr> | <tr> | ||
<td colspan="4" class="my-colspan4">Receptor expression</td> | <td colspan="4" class="my-colspan4">Receptor expression</td> | ||
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</td> | </td> | ||
<td>T1R2</td> | <td>T1R2</td> | ||
| − | <td>Human | + | <td>Human Sweet-receptor</td> |
<td>2520</td> | <td>2520</td> | ||
</tr> | </tr> | ||
| Line 48: | Line 48: | ||
</td> | </td> | ||
<td>T1R3</td> | <td>T1R3</td> | ||
| − | <td>Human | + | <td>Human Sweet-receptor</td> |
<td>1989</td> | <td>1989</td> | ||
</tr> | </tr> | ||
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368007">BBa_2368007</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368007">BBa_2368007</a> | ||
</td> | </td> | ||
| − | <td>Gal 1/10-T1R2- | + | <td>P<sub>Gal 1/10</sub>-T1R2-CYC1t</td> |
| − | <td>Completed expression of the human | + | <td>Completed expression of the human Sweet-receptor</td> |
<td>3449</td> | <td>3449</td> | ||
</tr> | </tr> | ||
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368008">BBa_2368008</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368008">BBa_2368008</a> | ||
</td> | </td> | ||
| − | <td>Gal 1/10-T1R3-ADH1</td> | + | <td>P<sub>Gal 1/10</sub>-T1R3-ADH1</td> |
| − | <td>Completed expression of the human | + | <td>Completed expression of the human Sweet-receptor</td> |
<td>2814</td> | <td>2814</td> | ||
</tr> | </tr> | ||
| Line 73: | Line 73: | ||
<td>MYC+T1R2 overlap</td> | <td>MYC+T1R2 overlap</td> | ||
<td>Marker genes of the Sweet-receptor</td> | <td>Marker genes of the Sweet-receptor</td> | ||
| − | <td> | + | <td>349</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 142: | Line 142: | ||
| − | + | </tbody> | |
| + | <tbody style="background-color: rgba(0,255,0,0.1);"> | ||
<tr> | <tr> | ||
| − | <td colspan="4" style="line-height: 3rem; text-transform: uppercase; font-weight: bold;">Host | + | <td colspan="4" style="line-height: 3rem; text-transform: uppercase; font-weight: bold;">Host engineering</td> |
</tr> | </tr> | ||
| Line 155: | Line 156: | ||
</td> | </td> | ||
<td>500bp Homologous arm＋His</td> | <td>500bp Homologous arm＋His</td> | ||
| − | <td> | + | <td>Knock out the <i>sst2</i> gene</td> |
<td>2050</td> | <td>2050</td> | ||
</tr> | </tr> | ||
| Line 163: | Line 164: | ||
</td> | </td> | ||
<td>500bp Homologous arm＋Ura</td> | <td>500bp Homologous arm＋Ura</td> | ||
| − | <td> | + | <td>Knock out the <i>far1</i> gene</td> |
<td>2050</td> | <td>2050</td> | ||
</tr> | </tr> | ||
| Line 170: | Line 171: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368016">BBa_2368016</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368016">BBa_2368016</a> | ||
</td> | </td> | ||
| − | <td>500bp Homologous | + | <td>500bp Homologous arm＋Trp</td> |
| − | <td> | + | <td>Knock out the <i>ste2</i> gene</td> |
<td>2071</td> | <td>2071</td> | ||
</tr> | </tr> | ||
| Line 179: | Line 180: | ||
</td> | </td> | ||
<td>Modified Gα Schizosaccharomyces pombe</td> | <td>Modified Gα Schizosaccharomyces pombe</td> | ||
| − | <td>Signal | + | <td>Signal transduction</td> |
<td>1421</td> | <td>1421</td> | ||
</tr> | </tr> | ||
| Line 187: | Line 188: | ||
</td> | </td> | ||
<td>Modified Gα Caenorhabditis elegans</td> | <td>Modified Gα Caenorhabditis elegans</td> | ||
| − | <td>Signal | + | <td>Signal transduction</td> |
<td>1418</td> | <td>1418</td> | ||
</tr> | </tr> | ||
| Line 195: | Line 196: | ||
</td> | </td> | ||
<td>Gα</td> | <td>Gα</td> | ||
| − | <td>Signal | + | <td>Signal transduction</td> |
<td>1421</td> | <td>1421</td> | ||
</tr> | </tr> | ||
| − | + | </tbody> | |
| + | <tbody style="background-color: rgba(0,180,255,0.1);"> | ||
<tr> | <tr> | ||
| − | <td colspan="4" style="line-height: 2rem; text-transform: uppercase; font-weight: bold;"> | + | <td colspan="4" style="line-height: 2rem; text-transform: uppercase; font-weight: bold;">Detection circuit</td> |
</tr> | </tr> | ||
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368005">BBa_2368005</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368005">BBa_2368005</a> | ||
</td> | </td> | ||
| − | <td> | + | <td><i>P<sub>fus</sub></i></td> |
<td>Induced promoter</td> | <td>Induced promoter</td> | ||
<td>438</td> | <td>438</td> | ||
| Line 218: | Line 220: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368026">BBa_2368026</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368026">BBa_2368026</a> | ||
</td> | </td> | ||
| − | <td> | + | <td><i>P<sub>fus</sub></i>(4 STE12 binding sites)</td> |
| − | <td> | + | <td>Engineered promoter</td> |
<td>223</td> | <td>223</td> | ||
</tr> | </tr> | ||
| Line 226: | Line 228: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368027">BBa_2368027</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368027">BBa_2368027</a> | ||
</td> | </td> | ||
| − | <td> | + | <td><i>P<sub>fus</sub></i>(6 STE12 binding sites)</td> |
| − | <td> | + | <td>Engineered promoter</td> |
<td>249</td> | <td>249</td> | ||
</tr> | </tr> | ||
| Line 234: | Line 236: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368006">BBa_2368006</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368006">BBa_2368006</a> | ||
</td> | </td> | ||
| − | <td> | + | <td><i>P<sub>fus</sub></i>+mRFP+CYC1t</i></td> |
| − | <td>The | + | <td>The reporter</td> |
<td>1375</td> | <td>1375</td> | ||
</tr> | </tr> | ||
| Line 242: | Line 244: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368002">BBa_2368002</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368002">BBa_2368002</a> | ||
</td> | </td> | ||
| − | <td> | + | <td><i>CYC1t</i> </td> |
| − | <td>the yeast</td> | + | <td>A terminator in the yeast</td> |
<td>231</td> | <td>231</td> | ||
</tr> | </tr> | ||
| Line 250: | Line 252: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368029">BBa_2368029</a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2368029">BBa_2368029</a> | ||
</td> | </td> | ||
| − | <td> | + | <td> <i>P<sub>fus</sub></i>(2 STE12 binding sites)</td> |
| − | <td> | + | <td>Engineered promoter</td> |
<td>213</td> | <td>213</td> | ||
</tr> | </tr> | ||
| Line 260: | Line 262: | ||
| − | + | <div class="article-nav" style="width: 100%;"> | |
| − | + | <a href="https://2017.igem.org/Team:BIT-China/Medal" class="article-nav-right2"> | |
| − | + | <img class="article-caption" src="https://static.igem.org/mediawiki/2017/6/6f/T--BIT-China--2017next.png" alt=""> | |
| − | + | </a> | |
| − | + | <div style="clear: both;"></div> | |
| + | </div> | ||
</section> | </section> | ||
Latest revision as of 13:33, 1 November 2017
| Biobrick | Name | Description | Length |
|---|---|---|---|
| Receptor expression | |||
| BBa_2368003 | T1R2 | Human Sweet-receptor | 2520 |
| BBa_2368004 | T1R3 | Human Sweet-receptor | 1989 |
| BBa_2368007 | PGal 1/10-T1R2-CYC1t | Completed expression of the human Sweet-receptor | 3449 |
| BBa_2368008 | PGal 1/10-T1R3-ADH1 | Completed expression of the human Sweet-receptor | 2814 |
| BBa_2368023 | MYC+T1R2 overlap | Marker genes of the Sweet-receptor | 349 |
| BBa_2368009 | HIS+T1R2 overlap | Marker genes of the Sweet-receptor | 50 |
| BBa_2368024 | HIS+T1R3 overlap | Marker genes of the Sweet-receptor | 187 |
| BBa_2368011 | FLAG+T1R3 overlap | Marker genes of the Sweet-receptor | 50 |
| BBa_2368012 | YCP+T1R2 overlap | Marker genes of the Sweet-receptor | 740 |
| BBa_2368013 | YCP+T1R3 overlap | Marker genes of the Sweet-receptor | 740 |
| BBa_2368014 | BCP+T1R2 overlap | Marker genes of the Sweet-receptor | 710 |
| BBa_2368015 | GFP+T1R2 overlap | Marker genes of the Sweet-receptor | 699 |
| BBa_2368020 | BCP+T1R3 overlap | Marker genes of the Sweet-receptor | 710 |
| Host engineering | |||
| BBa_2368017 | 500bp Homologous arm＋His | Knock out the sst2 gene | 2050 |
| BBa_2368018 | 500bp Homologous arm＋Ura | Knock out the far1 gene | 2050 |
| BBa_2368016 | 500bp Homologous arm＋Trp | Knock out the ste2 gene | 2071 |
| BBa_2368021 | Modified Gα Schizosaccharomyces pombe | Signal transduction | 1421 |
| BBa_2368022 | Modified Gα Caenorhabditis elegans | Signal transduction | 1418 |
| BBa_2368028 | Gα | Signal transduction | 1421 |
| Detection circuit | |||
| BBa_2368005 | Pfus | Induced promoter | 438 |
| BBa_2368026 | Pfus(4 STE12 binding sites) | Engineered promoter | 223 |
| BBa_2368027 | Pfus(6 STE12 binding sites) | Engineered promoter | 249 |
| BBa_2368006 | Pfus+mRFP+CYC1t | The reporter | 1375 |
| BBa_2368002 | CYC1t | A terminator in the yeast | 231 |
| BBa_2368029 | Pfus(2 STE12 binding sites) | Engineered promoter | 213 |
