<p> At low cell density, the quantity of AHL produced is too little for it to bring about Latarcin production. As the cell density increases, the cells divide and simultaneously produce both AHL and LuxR. Without the AHL, LuxR is unstable and gets degraded shortly after production. But beyond a certain threshold level, AHL begins forming a complex with LuxR, preventing its degradation. This complex now binds to the Lux promoter that regulates Latarcin synthesis. On binding, the promoter immediately switches ON and begins to produce a large amount of Latarcin. A small portion of the synthesised Latarcin destroys the host, and the rest can now be isolated and purified. </p>

                       <p> At low cell density, the quantity of AHL produced is too little for it to bring about Latarcin production. As the cell density increases, the cells divide and simultaneously produce both AHL and LuxR. Without the AHL, LuxR is unstable and gets degraded shortly after production. But beyond a certain threshold level, AHL begins forming a complex with LuxR, preventing its degradation. This complex now binds to the Lux promoter that regulates Latarcin synthesis. On binding, the promoter immediately switches ON and begins to produce a large amount of Latarcin. A small portion of the synthesised Latarcin destroys the host, and the rest can now be isolated and purified. </p>

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   <p>  To serve as a nutrient source for the bacteria, LB broth is contained within a compartment made of PVC, which is sandwiched between the two PVDF membranes. When mechanical stress is applied, the delicate PVC membrane disintegrates, allowing the bacteria to come in contact with the LB broth. When this happens, the dormant bacteria start multiplying.  Once the bacteria reach a substantial number,  due to the quorum sensing mechanism, the mutant latarcin will be  synthesised. </p>

   <p>  To serve as a nutrient source for the bacteria, LB broth is contained within a compartment made of PVC, which is sandwiched between the two PVDF membranes. When mechanical stress is applied, the delicate PVC membrane disintegrates, allowing the bacteria to come in contact with the LB broth. When this happens, the dormant bacteria start multiplying.  Once the bacteria reach a substantial number,  due to the quorum sensing mechanism, the mutant latarcin will be  synthesised. </p>

<p>  This results in abundant production of Latarcin by the host bacteria.  Latarcin diffuses through the inner PVDF membrane and reaches the food. On encountering bacteria present on the food, Latarcin effectively kills them via the carpet mechanism. Because of its half life of 10 hours, any unused Latarcin is therefore degraded, making the food safe for human consumption. </p>

<p>  This results in abundant production of Latarcin by the host bacteria.  Latarcin diffuses through the inner PVDF membrane and reaches the food. On encountering bacteria present on the food, Latarcin effectively kills them via the carpet mechanism. Because of its half life of 10 hours, any unused Latarcin is therefore degraded, making the food safe for human consumption. </p>

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