Team:REC-CHENNAI/Notebook
Lab Journal
This is our first time in iGEM and the past couple of months has been nothing short of hectic.
Scroll down to read our timeline - a journey of nights that turned into days with lab partners that turned into family.
| alarm_on February | |
|---|---|
| event 15 | lightbulb_outline Finalized team |
| event 15 | lightbulb_outline Created a group on WhatsApp. After a lot of reading, we got an idea, something about Lactoferrin. |
| event15-20 | lightbulb_outline Reading reading Zzzz |
| event 20 | lightbulb_outline Team met to understand the registration process. |
| event 21 | lightbulb_outline Had a discussion and concluded that Lf is too big a protein to work with in this short time span. Dropped it. |
| event 22 | lightbulb_outline Back to square one. No project idea. Split ourselves into 3 groups, each focusing on a different problem statement. |
| alarm_on March & April | |
| lightbulb_outline Brainstorming and Fundraising. | |
| alarm_on May | |
| event 02 | lightbulb_outline Break for end semester exams. |
| event 03 | lightbulb_outline Whole team met Ramit. He agreed to mentor our team. |
| event 10 | lightbulb_outline Opened our iGEM kit. |
| event10-21 | lightbulb_outline Lab construction and renovation in progress. lightbulb_outlineMeanwhile literature work , brainstorming for project ideas. |
| event15-20 | lightbulb_outlineDecided to work with AMPs lightbulb_outlineCame across papers related to Latarcin lightbulb_outlineDecided to work with this particular peptide lightbulb_outlineLab construction and renovation in progress. |
| alarm_on June | |
| event01-09 | lightbulb_outline Brainstorming for applications. lightbulb_outline Lab construction and renovation in progress. |
| event10 | lightbulb_outlineFirst skype session with Carolina. lightbulb_outline Finalized our project idea. |
| event11 | lightbulb_outlineTeam was split into wet lab, interlab study and HP team. |
| event13 | lightbulb_outlineFirst team fight :P |
| event13-25 | lightbulb_outlinePlaced order for all chemicals and kits required. |
| event26 | lightbulb_outlineMet Dr. N.G.Iboyaima Singh and Dr. G.R.Gopi to discuss our Delivery system. |
| event27 | lightbulb_outlineTeam meeting to discuss about mutant latarcin. lightbulb_outlineSomething seemed off. We realized that people were still not comfortable working with each other. Decided to talk it out like adults :P |
| event28 | lightbulb_outlineTeam bonding time. Played basketball. Went out for lunch. |
| event29-30 | lightbulb_outlinePreparing buffers. |
| alarm_on July | |
| event02 | lightbulb_outlineCompetent cell preparation. |
| event03 | lightbulb_outlineTransformation of plasmid from the iGEM kit. |
| event05 | lightbulb_outlineNo growth, Failed. |
| event06-17 | lightbulb_outlineAlter protocol- prepare competent cells- transform- fail terribly- repeat. |
| event15-16 | lightbulb_outlineMarcia & Nishka attended the All India iGEM Meet at IISER, Pune. |
| event20 | lightbulb_outlineGot our first transformation results. |
| event26 | lightbulb_outlineConducted a workshop for school students. |
| event27-30 | lightbulb_outlineDesigned and ordered our gblocks. |
| alarm_on August | |
| event01-12 | lightbulb_outlinePlasmid isolation and restriction digestion optimisation. lightbulb_outlineWorked on improving our transformation efficiency. |
| event13 | lightbulb_outlineConducted Biofair with SVCE. Turned out pretty well despite the fluctuating weather. |
| event14-30 | lightbulb_outlinePlaced order for our primers. lightbulb_outlineStarted working on the wiki. lightbulb_outlineWorked on our questionnaire for the HP. |
| alarm_on September | |
| event02 | lightbulb_outlineLigation refused to work out. lightbulb_outlineDecided to move on to interlab study. |
| event04-20 | lightbulb_outlineInterlab study. |
| event21-24 | lightbulb_outlineBack to working on ligation in the lab. |
| event25-26 | lightbulb_outlineUS VISA interview.VISA approved! |
| event27 | lightbulb_outlineSkype session with UCopenhagen about Podcasts. |
| event28-30 | lightbulb_outlineCompilation of HP work. |
| alarm_on October | |
| event03-10 | lightbulb_outlineRestriction-Ligation-Transformation. |
| event11-12 | lightbulb_outlineWorked on Podcasts. |
| event13-20 | lightbulb_outlineRealized that Jamboree Preparation was way overdue! lightbulb_outlineStarted working on our Poster and Presentation. |
| event21-30 | lightbulb_outlinePresentation practice. lightbulb_outlineFinal touches on Wiki. lightbulb_outlineGet ready for the Jamboree. |
| alarm_on November | |
| event01-07 | lightbulb_outlineStudy-Crib-Write semester exam-Cry.Repeat. |
