We carried out insilico studies for both our peptides using ExPASy ProtParam tool and the results are as listed below.
Peptide Sequence: GLFGKLIKKFGRKAISYAVKKARGKH-OH
Number of amino acids: 26
Molecular weight: 2902.57
Theoretical pI: 11.33
Carbon |
C |
136 |
Hydrogen |
H |
229 |
Nitrogen |
N |
41 |
Oxygen |
O |
29 |
Sulfur |
S |
0 |
Formula: C136H229N41O29
Total number of atoms: 435
The N-terminal of the sequence considered is G (Gly).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
The instability index (II) is computed to be 1.29
This classifies the protein as stable.
Aliphatic index: 82.69
Grand average of hydropathicity (GRAVY): -0.438
Peptide sequence: GLFGKLIKKKGRKAISYAVKKARGKH-OH
Number of amino acids: 26
Molecular weight: 2883.57
Theoretical pI: 11.36
Carbon |
C |
133 |
Hydrogen |
H |
232 |
Nitrogen |
N |
42 |
Oxygen |
O |
29 |
Sulfur |
S |
0 |
Formula: C133H232N42O29
Total number of atoms: 436
The N-terminal of the sequence considered is G (Gly).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
The instability index (II) is computed to be -1.98
This classifies the protein as stable.
Aliphatic index: 82.69
Grand average of hydropathicity (GRAVY): -0.696