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− | <span class="image avatar"><a href="https://2017.igem.org/Team:TU_Darmstadt"><img src="https://static.igem.org/mediawiki/2017/3/3d/LogoOWL.png" alt="home" | + | <style> |
+ | @media screen and (max-width: 1024px){ | ||
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+ | #teaser { | ||
+ | background-color: #002d54; | ||
+ | height: 100%; | ||
+ | position: static; | ||
+ | top: 0; | ||
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+ | width: 100%; | ||
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+ | .center { | ||
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+ | border:3px solid #e4f1fe; | ||
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+ | border:5px dashed #d4043b; | ||
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+ | <span class="image avatar"><a href="https://2017.igem.org/Team:TU_Darmstadt"><img src="https://static.igem.org/mediawiki/2017/3/3d/LogoOWL.png" alt="home"></a></span> | ||
<h1 id="logo"><a href="https://2017.igem.org/Team:TU_Darmstadt">ChiTUcare</a></h1> | <h1 id="logo"><a href="https://2017.igem.org/Team:TU_Darmstadt">ChiTUcare</a></h1> | ||
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<li><a href="https://2017.igem.org/Team:TU_Darmstadt/project">Project</a></li> | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/project">Project</a></li> | ||
<li><a href="https://2017.igem.org/Team:TU_Darmstadt/human_practices">Human Practices</a></li> | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/human_practices">Human Practices</a></li> | ||
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<li><a href="https://2017.igem.org/Team:TU_Darmstadt/team">Team</a></li> | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/team">Team</a></li> | ||
<li><a href="https://2017.igem.org/Team:TU_Darmstadt/judging">Judging</a></li> | <li><a href="https://2017.igem.org/Team:TU_Darmstadt/judging">Judging</a></li> | ||
− | </ul> | + | </div></ul> |
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+ | <img src="https://static.igem.org/mediawiki/2017/6/63/T--TU_Darmstadt--BannerHomePage5.png" style="width:100%;height:auto;"/> | ||
<div class="container"> | <div class="container"> | ||
− | <header class="major"> | + | <!--<header class="major"> |
− | <h2>ChiTUcare</h2> | + | <center><h2 class="projectTitle">ChiTUcare</h2></center> |
− | </header> | + | </header>--> |
− | <div class="post-it"> | + | <!--<div class="post-it"> |
− | <p style=" | + | <p>...</p></div>--> |
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+ | {{TU_Darmstadt/Flowchart}} | ||
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− | <section id="two"><div class="container"> | + | |
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+ | <section id="teaser"> | ||
+ | <br><br> | ||
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+ | <div class="box alt"> | ||
+ | <center> <a href="https://2017.igem.org/Team:TU_Darmstadt/Demonstrate"> | ||
+ | <h2 class="HoverText"> Proof of Concept </h2></a> </center> | ||
+ | <div class="row 50% uniform"> | ||
+ | <div class="1u"></div> | ||
+ | <div class="4u"><span class="image fit"><a href="https://2017.igem.org/Team:TU_Darmstadt/project/hydrogel"><img src="https://static.igem.org/mediawiki/2017/e/e3/T--TU_Darmstadt--Teaser_Pic4.png" style="border-radius: 50%;" class="HoverBorder" alt=""/></a></span></div> | ||
+ | <div class="2u"></div> | ||
+ | <div class="4u"><span class="image fit"><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase"><img src="https://static.igem.org/mediawiki/2017/d/df/T--TU_Darmstadt--Teaser_Pic3.png" style="border-radius: 50%;" class="HoverBorder" alt="" /></a></span></div> | ||
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+ | <div class="6u"><span class="image fit"><video src="https://static.igem.org/mediawiki/2017/5/56/T--TU_Darmstadt--geiles_video.mp4" alt="Chitosan-Alkaline production" style=" | ||
+ | border:3px solid #e4f1fe; width:100%; position:relative; | ||
+ | z-index: 2; margin-bottom: -26%; margin-top: -26%;" controls></video> </span></div> | ||
+ | <div class="3u"></div> | ||
+ | </div> | ||
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+ | <div class="4u"><span class="image fit"><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chitin_deacetylase"><img src="https://static.igem.org/mediawiki/2017/c/cf/T--TU_Darmstadt--Teaser_Pic2.png" style="border-radius: 50%;" class="HoverBorder" alt="" /></a></span></div> | ||
+ | <div class="2u"></div> | ||
+ | <div class="4u"><span class="image fit"><a href="https://2017.igem.org/Team:TU_Darmstadt/project/chemistry"><img src="https://static.igem.org/mediawiki/2017/d/db/T--TU_Darmstadt--Teaser_Pic1.png" style="border-radius: 50%;" class="HoverBorder" alt="" /></a></span></div> | ||
+ | <div class="1u"></div> | ||
+ | </div> | ||
+ | </div> | ||
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+ | <br><br> | ||
+ | </section> | ||
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+ | <!--<section id="two"><div class="container"> | ||
<p>Chitosan is a biopolymer with both antibacterial and wound-healing properties. By linking fluorophores to chitosan oligomeres smart plasters can be produced, able to detect pathogenic bacteria via proteolytic activity. Therefore, production of designed chitosan for medical purpose is of special interest.</p> | <p>Chitosan is a biopolymer with both antibacterial and wound-healing properties. By linking fluorophores to chitosan oligomeres smart plasters can be produced, able to detect pathogenic bacteria via proteolytic activity. Therefore, production of designed chitosan for medical purpose is of special interest.</p> | ||
<center><img src="https://static.igem.org/mediawiki/2017/3/36/TUDarmstadtPDplaster.png" alt="chitosan hydrogel" width="80%" style="padding: 1em 0 1em 0;"></center> | <center><img src="https://static.igem.org/mediawiki/2017/3/36/TUDarmstadtPDplaster.png" alt="chitosan hydrogel" width="80%" style="padding: 1em 0 1em 0;"></center> | ||
− | <h4>Engineering <i>E. coli</i> for specific | + | <h4>Engineering <i>E. coli</i> for specific Synthesis of Designer Chitosan</h4> |
<p>Our primary wet-lab goal is to engineer a synthetic biological circuit for the specific synthesis of chitosans in <i>E. coli</i>. The chemical properties as well as the bioactivity of chitosans mainly depend on three variables: the length of the oligomers, their level of deacetylation and their patterns of deacetylation.</p> | <p>Our primary wet-lab goal is to engineer a synthetic biological circuit for the specific synthesis of chitosans in <i>E. coli</i>. The chemical properties as well as the bioactivity of chitosans mainly depend on three variables: the length of the oligomers, their level of deacetylation and their patterns of deacetylation.</p> | ||
<p>Our enzymatic approach includes three enzymes. A chitin synthase (<i>Rhizobium leguminosarum bv. viciae</i>) catalyzes the oligomerization of N-acetylglucosamine-UDP monomers to chitin oligomers (tetramers and pentamers). Furthermore, two chitin deacetylases that differ in their regioselectivity (<i>Sinorhizobium meliloti</i> (nodB) and <i>Puccina graminis f. sp. tritici</i>) are regulated orthogonally, making it possible to choose between two different types of deacetylation patterns.</p> | <p>Our enzymatic approach includes three enzymes. A chitin synthase (<i>Rhizobium leguminosarum bv. viciae</i>) catalyzes the oligomerization of N-acetylglucosamine-UDP monomers to chitin oligomers (tetramers and pentamers). Furthermore, two chitin deacetylases that differ in their regioselectivity (<i>Sinorhizobium meliloti</i> (nodB) and <i>Puccina graminis f. sp. tritici</i>) are regulated orthogonally, making it possible to choose between two different types of deacetylation patterns.</p> | ||
<center><img src="https://static.igem.org/mediawiki/2017/2/2d/TUDarmstadtPDchitosansynthesis.png" alt="pathway chitosan" width="80%" style="padding: 1em 0 1em 0;"></center> | <center><img src="https://static.igem.org/mediawiki/2017/2/2d/TUDarmstadtPDchitosansynthesis.png" alt="pathway chitosan" width="80%" style="padding: 1em 0 1em 0;"></center> | ||
− | <h4>Application of | + | <h4>Application of Chitosan Oligomers: Chitosan Hydrogel for bacterial Enzyme Detection</h4> |
<p>Downstream of the synthetic biological circuit for the synthesis of chitosan pentamers, we want to give an example for an explicit application of chitosan oligomers. This shall be accomplished by building a plaster for wounds carrying the chitosan hydrogel with chitosan oligomers linked to a fluorophore via a peptide chain. When the plaster is applied to a wound it can detect bacterial protease activities and thus diagnose wound infection. Proteases will cleave the peptide linker and release the fluorophore, that is then detectable via UV-light.</p> | <p>Downstream of the synthetic biological circuit for the synthesis of chitosan pentamers, we want to give an example for an explicit application of chitosan oligomers. This shall be accomplished by building a plaster for wounds carrying the chitosan hydrogel with chitosan oligomers linked to a fluorophore via a peptide chain. When the plaster is applied to a wound it can detect bacterial protease activities and thus diagnose wound infection. Proteases will cleave the peptide linker and release the fluorophore, that is then detectable via UV-light.</p> | ||
<center><img src="https://static.igem.org/mediawiki/2017/4/40/TUDarmstadtPDpeptidelinker.png" alt="peptide linker" width="50%" style="padding: 1em 0 1em 0;"></center> | <center><img src="https://static.igem.org/mediawiki/2017/4/40/TUDarmstadtPDpeptidelinker.png" alt="peptide linker" width="50%" style="padding: 1em 0 1em 0;"></center> | ||
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<h3>Contact Us</h3> | <h3>Contact Us</h3> | ||
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Latest revision as of 20:27, 1 November 2017