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| − | {{UCC_Ireland}} | + | {{UCC_Ireland/Menu}} |
| | + | {{UCC_Ireland/Stylesheet}} |
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| | <html> | | <html> |
| | + | <style type="text/css"> |
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| − | <div class="column full_size" > | + | <head> |
| − | <img src="http://placehold.it/2000x300/d3d3d3/f2f2f2"> | + | <link href="https://2017.igem.org/Template:UCC_Ireland/CSS/style?action=raw&ctype=text/css" type="text/css" rel="stylesheet"> |
| − | </div> | + | <link href="https://fonts.googleapis.com/css?family=Saira" rel="stylesheet"> |
| | + | <title>UCC iGEM 2017</title> |
| | + | </head> |
| | + | <body> |
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| | + | <div class='content_main'> |
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| − | <div class="column full_size" > | + | <div style='margin:auto; width: 850px;'> |
| − | <h1> Welcome to iGEM 2017! </h1> | + | <img src='https://static.igem.org/mediawiki/2017/0/00/T--UCC_Ireland--moonshine_text_moo.png' style='width: 60%; margin: auto; display: block;'></img> |
| − | <p>Your team has been approved and you are ready to start the iGEM season! </p>
| + | </div> |
| − | </div> | + | |
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| − | <div class="clear"></div>
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| − | <div class="column half_size" > | + | <p id='home_page'> Our project aims to create a standardised universal biosensor strategy, that will be affordable and user friendly, containing all the components for readout. This system will consist of three elements: |
| − | <h5>Before you start: </h5> | + | <br/> |
| − | <p> Please read the following pages:</p> | + | <div class='abstract_container'> |
| − | <ul> | + | <div class='abstract_section'> |
| − | <li> <a href="https://2017.igem.org/Competition">Competition Hub</a> </li> | + | <center style="font-size:24px;font-weight:700;padding-bottom:5px;padding-top:0px;">Bioengineered construct</center> |
| − | <li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li> | + | <div class='home_imgdiv'> |
| − | <li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
| + | <img class="homepage_into_img" src='https://static.igem.org/mediawiki/2017/b/b2/T--UCC_Ireland--amilcp.png'> |
| − | </ul>
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| | </div> | | </div> |
| − | | + | A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein. |
| − | <div class="column half_size" >
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| − | <div class="highlight">
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| − | <h5> Styling your wiki </h5>
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| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| | </div> | | </div> |
| | + | <div class='abstract_section'> |
| | + | <center style="font-size:24px;font-weight:700;padding-bottom:5px;padding-top:0px;">Chassis</center> |
| | + | <div class='home_imgdiv'> |
| | + | <img class="homepage_into_img" src='https://static.igem.org/mediawiki/2017/d/d7/T--UCC_Ireland--plasmidhp.png'> |
| | </div> | | </div> |
| − | | + | The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system). |
| − | <div class="column full_size" >
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| − | <h5> Wiki template information </h5>
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| − | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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| − | </div>
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| − | <div class="column half_size" >
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| − | <h5> Editing your wiki </h5>
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| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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| − | <p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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| | </div> | | </div> |
| − | | + | <div class='abstract_section'> |
| − | | + | <center style="font-size:24px;font-weight:700;padding-bottom:5px;padding-top:0px;">Readout device</center> |
| − | <div class="column half_size" > | + | <div class='home_imgdiv'> |
| − | <h5>Tips</h5> | + | <img class="homepage_into_img" src='https://static.igem.org/mediawiki/2017/9/91/T--UCC_Ireland--phoneclipart.png'> |
| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <ul>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li> | + | |
| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li> | + | |
| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | </ul>
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| | </div> | | </div> |
| − | | + | The readout device was 3D printed and designed to attach easily to common smartphones. It uses spectrometry technology to measure the relative fluorescence or absorbance of a sample. We built an app to analyse the output from the device to determine the concentration of the substance being tested for. |
| − | | + | </div> |
| − | <div class="column half_size" >
| + | </div> |
| − | <h5>Inspiration</h5>
| + | <div style="height:50px;float:right;clear:right;width:100%;"></div> |
| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
| + | <div class='home_content'> |
| − | <ul>
| + | To validate the idea for our Universal Biosensor Strategy, we identified irish industries with a need for such a product. We have targeted the dairy and microbrewery industries as proof of concept for our project, to test for antibiotic or methanol residue. |
| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li> | + | |
| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li> | + | |
| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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| − | </ul>
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| | </div> | | </div> |
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| − | <div class="column half_size" > | + | <img src='https://static.igem.org/mediawiki/2017/5/58/T--UCC_Ireland--methanol_home_page.jpg' style='width: 90%; margin: auto; display: block;'></img> |
| − | <h5> Uploading pictures and files </h5>
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| − | <p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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| − | When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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| − | <a href="https://2017.igem.org/Special:Upload"> | + | <img src='https://static.igem.org/mediawiki/2017/5/53/T--UCC_Ireland--milk_home_page.jpg' style='width: 90%; margin: auto; display: block;'></img> |
| − | UPLOAD FILES
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| − | </a>
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| − | </p>
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| − | </div> | + | |
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| | + | </br> |
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| | + | </div> |
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| | + | </body> |
| | </html> | | </html> |
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| | + | {{UCC_Ireland/Footer}} |
Our project aims to create a standardised universal biosensor strategy, that will be affordable and user friendly, containing all the components for readout. This system will consist of three elements:
Bioengineered construct
A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein.
Chassis
The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system).
Readout device
The readout device was 3D printed and designed to attach easily to common smartphones. It uses spectrometry technology to measure the relative fluorescence or absorbance of a sample. We built an app to analyse the output from the device to determine the concentration of the substance being tested for.
To validate the idea for our Universal Biosensor Strategy, we identified irish industries with a need for such a product. We have targeted the dairy and microbrewery industries as proof of concept for our project, to test for antibiotic or methanol residue.
