Difference between revisions of "Team:UCC Ireland"

 
(246 intermediate revisions by 3 users not shown)
Line 1: Line 1:
 +
{{UCC_Ireland/Menu}}
 +
{{UCC_Ireland/Stylesheet}}
  
 +
<html>
 +
<style type="text/css">
 +
body {
  
−
<html lang="en" xmlns="http://www.w3.org/1999/html">
+
}
−
  <head>
+
</style>
−
    <meta charset="utf-8">
+
−
    <meta http-equiv="X-UA-Compatible" content="IE=edge">
+
−
    <meta name="viewport" content="width=device-width, initial-scale=1">
+
−
    <!-- The above 3 meta tags *must* come first in the head; any other head content must come *after* these tags -->
+
  
−
    <title>UCC iGEM</title>
+
<head>
 +
<link href="https://2017.igem.org/Template:UCC_Ireland/CSS/style?action=raw&ctype=text/css" type="text/css" rel="stylesheet">
 +
<link href="https://fonts.googleapis.com/css?family=Saira" rel="stylesheet">
 +
<title>UCC iGEM 2017</title>
 +
</head>
 +
<body>
  
−
    <!-- Bootstrap core CSS -->
+
<div class='content_main'>
−
    <link rel="stylesheet" href="https://2017.igem.org/Template:Team:UCC_Ireland/CSS?action=raw&amp;ctype=text/css">
+
  
−
    <script src="https://2016.igem.org/Template:Team:UCC_Ireland/JS?action=raw&amp;ctype=text/javascript"></script>
 
−
   
 
−
  </head>
 
  
−
  <body>
+
<div style='margin:auto; width: 850px;'>
−
    <div class="container-full">
+
<img src='https://static.igem.org/mediawiki/2017/0/00/T--UCC_Ireland--moonshine_text_moo.png' style='width: 60%; margin: auto; display: block;'></img>
−
      <nav class="navbar navbar-inverse navbar-fixed-top">
+
</div>
−
        <div class="container">
+
−
          <div class="navbar-header">
+
−
            <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false" aria-controls="navbar">
+
−
              <span class="sr-only">Toggle navigation</span>
+
−
              <span class="icon-bar"></span>
+
−
              <span class="icon-bar"></span>
+
−
              <span class="icon-bar"></span>
+
−
            </button>
+
−
              <a class="navbar-brand" href="https://2016.igem.org/Team:UCC_Ireland"><img style="height: 80px;" src="https://static.igem.org/mediawiki/2016/a/a3/LIMITLESSLACTIS-WHITE.png" alt=""></a>
+
−
          </div>
+
−
          <div id="navbar" class="navbar-collapse collapse">
+
−
            <ul class="nav navbar-nav">
+
−
              <li class="active"><a href="https://2016.igem.org/Team:UCC_Ireland">Home</a></li>
+
−
              <li class="dropdown">
+
−
                <a class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Project<span class="caret"></span></a>
+
−
                <ul class="dropdown-menu">
+
−
                  <li><a href="https://2016.igem.org/Team:UCC_Ireland/Limitless_Lactis">Limitless Lactis</a></li>
+
−
                  <li><a href="https://2016.igem.org/Team:UCC_Ireland/iRFP">iRFP In-Vitro Reporter</a></li>
+
−
                  <li><a href="https://2016.igem.org/Team:UCC_Ireland/Leishmaniasis">Leishmaniasis</a></li>
+
−
                  <li><a href="https://2016.igem.org/Team:UCC_Ireland/Proof">Proof</a></li>
+
−
                  <li><a href="https://2016.igem.org/Team:UCC_Ireland/Demonstrate">Demonstrate</a></li>
+
−
                </ul>
+
−
              </li>
+
−
              <li class="dropdown">
+
−
                <a class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Outreach<span class="caret"></span></a>
+
−
                <ul class="dropdown-menu">
+
−
                  <li><a href="https://2016.igem.org/Team:UCC_Ireland/HP/Silver">HP Silver</a></li>
+
−
                  <li><a href="https://2016.igem.org/Team:UCC_Ireland/HP/Gold">HP Gold</a></li>
+
−
                  <li role="separator" class="divider"></li>
+
−
                  <li><a href="https://2016.igem.org/Team:UCC_Ireland/Engagement">Engagement</a></li>
+
−
                  <li><a href="https://2016.igem.org/Team:UCC_Ireland/Integrated_Practices">Integrated Practices</a></li>
+
−
                </ul>
+
−
              </li>
+
−
              <li><a href="https://2016.igem.org/Team:UCC_Ireland/Team">Team</a></li>
+
−
              <li><a href="https://2016.igem.org/Team:UCC_Ireland/Notebook">Notes</a></li>
+
−
              <li><a href="https://2016.igem.org/Team:UCC_Ireland/Parts">Parts</a></li>
+
−
              <li class="dropdown">
+
−
                <a class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Collaborations<span class="caret"></span></a>
+
−
                <ul class="dropdown-menu">
+
−
                  <li><a href="https://2016.igem.org/Team:UCC_Ireland/Collaborations">iGEM Teams</a></li>
+
−
                  <li role="separator" class="divider"></li>
+
−
                  <li class="dropdown-header">Synenergene</li>
+
−
                  <li><a href="https://2016.igem.org/Team:UCC_Ireland/Application_Scenario">Application Scenario</a></li>
+
−
                  <li><a href="https://2016.igem.org/Team:UCC_Ireland/Techno_Moral_Newsletter">Techno-Moral Newsletter</a></li>
+
−
                  <li><a href="https://2016.igem.org/Team:UCC_Ireland/Blog.html">Techno-Moral Blog</a></li>
+
−
                </ul>
+
−
              </li>
+
−
              <li><a href="https://2016.igem.org/Team:UCC_Ireland/Interlab_Study">Interlab</a></li>
+
−
              <li><a href="https://2016.igem.org/Team:UCC_Ireland/Achievements">Achievements</a></li>
+
−
              <li><a href="https://2016.igem.org/Team:UCC_Ireland/Attributions">Attributions</a></li>
+
−
            </ul>
+
−
          </div>
+
−
        </div>
+
−
      </nav>
+
−
      <div class="container-fluid fix-nav-padding-top">
+
  
−
        <div class="row" id="landing">
 
−
          <div class="col-xs-12 col-md-12">
 
  
−
            <img style="width: 400px; display:block; margin:auto;" src="https://static.igem.org/mediawiki/2016/6/6b/T--UCC_Ireland--LIMITLESSLACTIS-BLACK.png" alt="">
+
<p id='home_page'> Our project aims to create a standardised universal biosensor strategy, that will be affordable and user friendly, containing all the components for readout. This system will consist of three elements:
−
           
+
<br/>
−
          </div>
+
<div class='abstract_container'>
−
        </div><!-- /landing -->
+
<div class='abstract_section'>
−
       
+
<center style="font-size:24px;font-weight:700;padding-bottom:5px;padding-top:0px;">Bioengineered construct</center>
 +
<div class='home_imgdiv'>
 +
<img class="homepage_into_img" src='https://static.igem.org/mediawiki/2017/b/b2/T--UCC_Ireland--amilcp.png'>
 +
</div>
 +
A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein.
 +
</div>
 +
<div class='abstract_section'>
 +
<center style="font-size:24px;font-weight:700;padding-bottom:5px;padding-top:0px;">Chassis</center>
 +
<div class='home_imgdiv'>
 +
<img class="homepage_into_img" src='https://static.igem.org/mediawiki/2017/d/d7/T--UCC_Ireland--plasmidhp.png'>
 +
</div>
 +
The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system).
 +
</div>
 +
<div class='abstract_section'>
 +
<center style="font-size:24px;font-weight:700;padding-bottom:5px;padding-top:0px;">Readout device</center>
 +
<div class='home_imgdiv'>
 +
<img class="homepage_into_img" src='https://static.igem.org/mediawiki/2017/9/91/T--UCC_Ireland--phoneclipart.png'>
 +
</div>
 +
The readout device was 3D printed and designed to attach easily to common smartphones. It uses spectrometry technology to measure the relative fluorescence or absorbance of a sample. We built an app to analyse the output from the device to determine the concentration of the substance being tested for.
 +
</div>
 +
</div>
 +
<div style="height:50px;float:right;clear:right;width:100%;"></div>
 +
<div class='home_content'>
 +
To validate the idea for our Universal Biosensor Strategy, we identified irish industries with a need for such a product. We have targeted the dairy and microbrewery industries as proof of concept for our project, to test for antibiotic or methanol residue.
 +
</div>
  
−
        <div class="row text-center" id="abstract">
+
<img src='https://static.igem.org/mediawiki/2017/5/58/T--UCC_Ireland--methanol_home_page.jpg' style='width: 90%; margin: auto; display: block;'></img>
−
          <div class="col-xs-12 col-md-8 col-md-offset-2">
+
−
            <h2 class="text-center"><span>Limitless Lactis:</span> Intelligent <em>Lactococcus lactis</em> strains as a protein delivery platform for disease treatment.</h2>
+
−
    <hr>
+
−
              <p class="lead text-left">
+
−
              <em>Lactococcus lactis</em>, a generally recognised as safe (GRAS) bacterium commonly used in food production, is highly amenable to genetic manipulation. We aim to develop a synthetic <em>L. lactis</em>-based platform that can deliver proteins to immune cells to precisely influence the host immune response for use in the treatment of various diseases. Potential applications which we have investigated include vaccination strategies and macrophage modification. We have developed the platform as a vaccine against leishmaniasis, a neglected tropical disease increasing in geographical distribution. LJM11 is an immunogenic salivary protein of the sandfly vector, <em>Lutzomyia longipalpis</em>. Our inexpensive platform, through simple oral administration, has the capacity to deliver this protein to antigen-presenting cells, and potentially immunise against the life cycle of leishmaniasis. Besides vaccination strategies, this platform may be employed to modify the phenotype of other phagocytic cells associated with diseases such as cancer.
+
−
            </p>
+
−
              <hr>
+
−
              <div align="center" class="embed-responsive embed-responsive-16by9">
+
−
                <video controls class="embed-responsive-item">
+
−
                  <source src="https://static.igem.org/mediawiki/2016/e/e3/T--UCC_Ireland--I-Like-It-Lactis-UCC-iGEM-2016-SONG.mp4" type="video/mp4">
+
−
                </video>
+
−
              </div>
+
−
          </div>
+
−
        </div><!-- /abstract -->
+
−
        <br>
+
−
        <br>
+
−
        <br>
+
−
        <br>
+
−
        <hr>
+
  
−
        <div class="row" id="sponsors">
+
<img src='https://static.igem.org/mediawiki/2017/5/53/T--UCC_Ireland--milk_home_page.jpg' style='width: 90%; margin: auto; display: block;'></img>
−
          <div class="col-xs-12 col-md-11 col-md-11 text-center text-uppercase">
+
−
            <div class="row">
+
−
  <div class="col-xs-4 col-md-1 col-lg-1">
+
−
    <a href="http://apc.ucc.ie/" target="_blank"><img src="https://static.igem.org/mediawiki/2016/f/f3/T--UCC_Ireland--apc.png" alt=""></a>
+
−
  </div>
+
−
  <div class="col-xs-4 col-md-1 col-lg-1">
+
−
    <a href="http://www.ccrc.ie/" target="_blank"><img src="https://static.igem.org/mediawiki/2016/a/a6/T--UCC_Ireland--ccrc.png" alt=""></a>
+
−
  </div>
+
−
  <div class="col-xs-4 col-md-1 col-lg-1">
+
−
    <a href="https://www.ucc.ie/en/med-health/" target="_blank"><img src="https://static.igem.org/mediawiki/2016/2/20/T--UCC_Ireland--COMH.jpg" alt=""></a>
+
−
  </div>
+
−
  <div class="col-xs-4 col-md-1 col-lg-1">
+
−
    <a href="http://www.fyffes.us/" target="_blank"><img src="https://static.igem.org/mediawiki/2016/f/f8/T--UCC_Ireland--Fyffes_SVG_logo.svg.png" alt=""></a>
+
−
  </div>
+
−
  <div class="col-xs-4 col-md-1 col-lg-1">
+
−
    <a href="http://www.genscript.com/" target="_blank"><img src="https://static.igem.org/mediawiki/2016/5/5b/T--UCC_Ireland--genscripteditkevin.png" alt=""></a>
+
−
  </div>
+
−
  <div class="col-xs-4 col-md-1 col-lg-1">
+
−
    <a href="http://www.lilly.ie/en/index.aspx" target="_blank"><img src="https://static.igem.org/mediawiki/2016/7/73/T--UCC_Ireland--hutchinsonlilly-2vd3ujlywuaxuzb5zmonpc.jpg" alt=""></a>
+
−
  </div>
+
−
  <div class="col-xs-4 col-md-1 col-lg-1">
+
−
    <a href="http://eu.idtdna.com/site" target="_blank"><img src="https://static.igem.org/mediawiki/2016/b/b0/T--UCC_Ireland--IDT_logo_trans.png" alt=""></a>
+
−
  </div>
+
−
  <div class="col-xs-4 col-md-1 col-lg-1">
+
−
    <a href="http://www.janssen.com/ireland/" target="_blank"><img src="https://static.igem.org/mediawiki/2016/6/65/T--UCC_Ireland--Janssen.jpg" alt=""></a>
+
−
  </div>
+
−
  <div class="col-xs-4 col-md-1 col-lg-1">
+
−
    <a href="https://www.synenergene.eu/" target="_blank"><img src="https://static.igem.org/mediawiki/2016/e/ef/T--UCC_Ireland--logo%281%29.png" alt=""></a>
+
−
  </div>
+
−
  <div class="col-xs-4 col-md-1 col-lg-1">
+
−
    <a href="https://www.ucc.ie/en/biochemistry/" target="_blank"><img src="https://static.igem.org/mediawiki/2016/0/03/T--UCC_Ireland--schoolofbiochemlogoeditkevin.png" alt=""></a>
+
−
  </div>
+
−
  <div class="col-xs-4 col-md-1 col-lg-1">
+
−
    <a href="http://www.ucc.ie/en/" target="_blank"><img src="https://static.igem.org/mediawiki/2016/4/47/T--UCC_Ireland--ucc.png" alt=""></a>
+
−
  </div>
+
−
</div>
+
−
            <br>
+
−
            <br>
+
−
            <br>
+
−
            <br>
+
−
          </div>
+
−
        </div><!-- /sponsors -->
+
−
            <br>
+
−
            <br>
+
−
            <br>
+
−
            <br>
+
−
          </div>
+
−
        </div><!-- /sponsors -->
+
  
−
        <hr>
 
  
−
      </div><!-- /home -->
+
</br>
−
    </div> <!-- /container full -->
+
  
−
  </body>
+
 
 +
 
 +
</div>
 +
 
 +
 
 +
</body>
 
</html>
 
</html>
 +
 +
{{UCC_Ireland/Footer}}

Latest revision as of 23:02, 1 November 2017

UCC iGEM 2017

Our project aims to create a standardised universal biosensor strategy, that will be affordable and user friendly, containing all the components for readout. This system will consist of three elements:

Bioengineered construct
A biological circuit designed to detect the presence of a specific substrate. The sensing element is specific to the substance being detected, while the readout for each construct is AmilCP, a blue coloured Chromoprotein.
Chassis
The system is deployed initially through plasmid containing E. coli (tube-based), with the aim of progressing to a cell free system (as a lyophilised paper system).
Readout device
The readout device was 3D printed and designed to attach easily to common smartphones. It uses spectrometry technology to measure the relative fluorescence or absorbance of a sample. We built an app to analyse the output from the device to determine the concentration of the substance being tested for.
To validate the idea for our Universal Biosensor Strategy, we identified irish industries with a need for such a product. We have targeted the dairy and microbrewery industries as proof of concept for our project, to test for antibiotic or methanol residue.