Difference between revisions of "Team:FAFU-CHINA"

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{{FAFU-CHINA}}
 
{{FAFU-CHINA}}
 +
{{FAFU-CHINA/Bootstrap.js}}
 +
{{FAFU-CHINA/Bootstrap.css}}
 
<html>
 
<html>
 +
<head><meta name="viewport" content="width=device-width, initial-scale=1.0, minimum-scale=0.5, maximum-scale=2.0, user-scalable=yes" />   
 +
<meta name="apple-mobile-web-app-capable" content="yes" />   
 +
<meta name="format-detection" content="telephone=no" /> </head>
 +
<body>
  
 +
<style>
 +
body{
 +
opacity: 0.9;
 +
    background-color: #F1e6ce
 +
;
 +
 +
}
  
−
<div class="column full_size" >
+
.imagestyle{
−
<img src="http://placehold.it/2000x300/d3d3d3/f2f2f2">
+
width: 100%;
−
</div>
+
height: 500px;
 +
top:30px;
 +
}
 +
.introducetext{
 +
      margin-top: 8px;
 +
    margin-left: 44%;
 +
    font-style: italic;
 +
    color: #BC464C;
 +
    margin-top:77px;
  
 +
}
 +
.introducetext2{
 +
    width: 80%;
 +
    padding: 20px;
 +
    margin: 12px;
 +
    background-color: #D0E090;
 +
    border: 15px solid #BC464C;
 +
    border-style: initial;
 +
    margin-top: 30px;
 +
    font-size: 18px;
 +
    margin:0 auto;
 +
    margin-top:20px;
 +
    border-radius:20px 20px 20px 20px
 +
}
  
 +
.imagestyle1{
 +
    width: 396px;
 +
    height: 400px;
 +
    margin-left: 28%;
 +
}
 +
.lastpart{
 +
    height: 300px;
 +
}
  
−
<div class="column full_size" >
+
.logoo2{
−
<h1> Welcome to iGEM 2017! </h1>
+
      float:left;
−
<p>Your team has been approved and you are ready to start the iGEM season! </p>
+
      width:200px;
−
</div>
+
    }
 +
.logimg{
 +
    /*height: 50%;*/
 +
    width: 143%;
 +
    margin-top: -139%;}
 +
 +
.logoo1{
 +
    height:auto;
 +
    width: 200px;
 +
    float: right;
 +
    margin-top: -207px}
 +
.logoo1 img{
 +
    width: 60%;
 +
    height: 60%;
 +
    /*margin-top: -6%*/
 +
    }
  
−
<div class="clear"></div>
 
  
−
<div class="column half_size" >
+
.lasttupian{width: 80%;
−
<h5>Before you start: </h5>
+
margin-top:93px;
−
<p> Please read the following pages:</p>
+
border-radius:20px
−
<ul>
+
}
−
<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
+
.zuobain{
−
<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
+
float:left;
−
<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
+
width: 30%
−
</ul>
+
}
−
</div>
+
</style>
  
−
<div class="column half_size" >
 
−
<div class="highlight">
 
−
<h5> Styling your wiki </h5>
 
−
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 
−
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 
−
</div>
 
−
</div>
 
  
−
<div class="column full_size" >
 
−
<h5> Wiki template information </h5>
 
−
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
  
−
</div>  
+
<style>
 +
  .navbar-default {
 +
    background-color: #93bd46;
 +
    border-color: #6a8d2c;
 +
  }
 +
  .navbar-default .navbar-brand {
 +
    color: #e9f9e7 !important;
 +
  }
 +
  .navbar-default .navbar-brand:hover,
 +
  .navbar-default .navbar-brand:focus {
 +
    color: #ffffff;
 +
  }
 +
  .navbar-default .navbar-text {
 +
    color: #e9f9e7 !important;
 +
  }
 +
  .navbar-default .navbar-nav > li > a {
 +
    color: #e9f9e7 !important;
 +
  }
 +
  .navbar-default .navbar-nav > li > a:hover,
 +
  .navbar-default .navbar-nav > li > a:focus {
 +
    color: #ffffff !important;
 +
  }
 +
  .navbar-default .navbar-nav > li > .dropdown-menu {
 +
    background-color: #93bd46;
 +
  }
 +
  .navbar-default .navbar-nav > li > .dropdown-menu > li > a {
 +
    color: #e9f9e7 !important;
 +
  }
 +
  .navbar-default .navbar-nav > li > .dropdown-menu > li > a:hover,
 +
  .navbar-default .navbar-nav > li > .dropdown-menu > li > a:focus {
 +
    color: #ffffff !important;
 +
    background-color: #6a8d2c;
 +
  }
 +
  .navbar-default .navbar-nav > li > .dropdown-menu > li.divider {
 +
    background-color: #6a8d2c;
 +
  }
 +
  .navbar-default .navbar-nav .open .dropdown-menu > .active > a,
 +
  .navbar-default .navbar-nav .open .dropdown-menu > .active > a:hover,
 +
  .navbar-default .navbar-nav .open .dropdown-menu > .active > a:focus {
 +
    color: #ffffff !important;
 +
    background-color: #6a8d2c;
 +
  }
 +
  .navbar-default .navbar-nav > .active > a,
 +
  .navbar-default .navbar-nav > .active > a:hover,
 +
  .navbar-default .navbar-nav > .active > a:focus {
 +
    color: #ffffff !important;
 +
    background-color: #6a8d2c;
 +
  }
 +
  .navbar-default .navbar-nav > .open > a,
 +
  .navbar-default .navbar-nav > .open > a:hover,
 +
  .navbar-default .navbar-nav > .open > a:focus {
 +
    color: #ffffff !important;
 +
    background-color: #6a8d2c;
 +
  }
 +
  .navbar-default .navbar-toggle {
 +
    border-color: #6a8d2c;
 +
  }
 +
  .navbar-default .navbar-toggle:hover,
 +
  .navbar-default .navbar-toggle:focus {
 +
    background-color: #6a8d2c;
 +
  }
 +
  .navbar-default .navbar-toggle .icon-bar {
 +
    background-color: #e9f9e7 !important;
 +
  }
 +
  .navbar-default .navbar-collapse,
 +
  .navbar-default .navbar-form {
 +
    border-color: #e9f9e7 !important;
 +
  }
 +
  .navbar-default .navbar-link {
 +
    color: #e9f9e7 !important;
 +
  }
 +
  .navbar-default .navbar-link:hover {
 +
    color: #ffffff !important;
 +
  }
  
 +
  @media (max-width: 767px) {
 +
    .navbar-default .navbar-nav .open .dropdown-menu > li > a {
 +
    color: #e9f9e7 !important;
 +
    }
 +
    .navbar-default .navbar-nav .open .dropdown-menu > li > a:hover,
 +
    .navbar-default .navbar-nav .open .dropdown-menu > li > a:focus {
 +
    color: #ffffff !important;
 +
    }
 +
    .navbar-default .navbar-nav .open .dropdown-menu > .active > a,
 +
    .navbar-default .navbar-nav .open .dropdown-menu > .active > a:hover,
 +
    .navbar-default .navbar-nav .open .dropdown-menu > .active > a:focus {
 +
    color: #ffffff !important;
 +
    background-color: #6a8d2c;
 +
    }
 +
  }
 +
  .dropdown-menu li a{
 +
    background-color: #93bd46;
 +
  }
 +
  .dropdown-menu .active a{
 +
    background-color:#6a8d2c;
 +
  }
  
 +
 
 +
  body,html{
 +
    padding:0;
 +
    margin:0;
 +
  }
 +
  .dropdown ul li,.dropdown ul{
 +
    background-color:gainsboro;
 +
  }
 +
  .navbar .nav > li .dropdown-menu { 
 +
    margin: 0; 
 +
  } 
 +
  .navbar .nav > li:hover .dropdown-menu { 
 +
    display: block; 
 +
  }
 +
  .noma{
 +
    width:70%;
 +
    margin:0 auto;
 +
    padding:0;
 +
  }
  
  
−
<div class="column half_size" >
+
</style>
−
<h5> Editing your wiki </h5>
+
<nav class="navbar navbar-default " style="margin-top:145px;z-index: 888">
−
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
+
  <div class="container-fluid noma">
−
<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
+
  
−
</div>
 
  
 +
    <!-- Collect the nav links, forms, and other content for toggling -->
 +
    <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
 +
<ul class="nav navbar-nav">
 +
<li class="active"><a href="#">Home</a></li>
  
−
<div class="column half_size" >
+
<li class="dropdown">
−
<h5>Tips</h5>
+
  <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Project <span class="caret"></span></a>
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
  <ul class="dropdown-menu">
−
<ul>
+
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Design">Design</a></li>
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Applied_Design">Background</a></li>
−
<li>Be clear about what you are doing and how you plan to do this.</li>
+
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/InterLab">Interlab</a></li>
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
<li class="chooseone"><a href="https://2017.igem.org/Team:FAFU-CHINA/Plant">Module 1: Phyto-route</a></li>
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
+
<li class="choosetwo"><a href="https://2017.igem.org/Team:FAFU-CHINA/Experiments#sss2">Module 2: Metal-trap</a></li>
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
+
<li class="choosethree"><a href="https://2017.igem.org/Team:FAFU-CHINA/Experiments#sss3">Module 3: Safeguard</a></li>
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
+
  </ul>
−
<li>Have lots of fun! </li>
+
</li>
−
</ul>  
+
<li class="dropdown">
−
</div>
+
  <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Achievements <span class="caret"></span></a>
 +
  <ul class="dropdown-menu">
 +
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Demonstrate">Demonstrate</a></li>
 +
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Judging_Form">Judging Form</a></li>
 +
<!--<li><a href="https://2017.igem.org/Team:Example2/Entrepreneurship">Community</a></li>-->
 +
  </ul>
 +
</li>
 +
<li class="dropdown">
 +
  <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Extras <span class="caret"></span></a>
 +
  <ul class="dropdown-menu">
 +
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Collaborations">Collaborations</a></li>
 +
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Protocols ">Protocols</a></li>
 +
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Notebook">Notebook</a></li>
 +
  </ul>
 +
</li>
 +
 +
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Parts">Parts</a></li>
 +
<!-- <li class="dropdown">
 +
  <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Results <span class="caret"></span></a>
 +
  <ul class="dropdown-menu">
 +
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Results">Overview</a></li>
 +
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Measurement">Measurement</a></li>
 +
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/HP/Gold_Integrated">Gold Integrated</a></li>
 +
<li><a href="https://2017.igem.org/Team:Example2/Entrepreneurship">Entrepreneurship</a></li>
 +
<li><a href="hhttps://2017.igem.org/Team:Example2/Software">Software</a></li>
 +
<li><a href="https://2017.igem.org/Team:Example2/Hardware">Hardware</a></li>
 +
<li><a href="https://2017.igem.org/Team:Example2/Plant">Plant</a></li>
 +
  </ul>
 +
</li>-->
 +
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Model">Model</a></li>
 +
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Human_Practices">Human Practice</a></li>
 +
<li class="dropdown">
 +
  <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Team <span class="caret"></span></a>
 +
  <ul class="dropdown-menu">
 +
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Team">Team members</a></li>
 +
<li><a href="https://2017.igem.org/Team:FAFU-CHINA/Attributions">Attributions</a></li>
 +
  </ul>
 +
</li>
 +
</ul>
 +
    </div><!-- /.navbar-collapse -->
 +
    </div><!-- /.navbar-collapse -->
 +
</nav>
 +
    <!--logo图片位置-->
 +
    <div class="logstyle">
 +
        <div class="logoo2"><img class="logimg" src="https://static.igem.org/mediawiki/2017/a/ae/T--FAFU-CHINA--006.jpg" ></div>
 +
        <div class="logoo1"><img src="https://static.igem.org/mediawiki/2017/c/c7/T--FAFU-CHINA--007.png" ></div>
 +
    </div>
 +
   
 +
  <div class="introducetext">
 +
            <h1 >
 +
          Overview
 +
          </h1>
 +
    </div>
 +
   
 +
    <div class="introducetext2">
 +
    <div><p  style="text-align:center; color: #6a8d2c ; font-size:25px;font-family:inherit;">Synergism of Phosphate-solubilizing microoranisms-plant</p>
 +
        <p style="text-align: center; color: #6a8d2c; font-size:25px;font-family: inherit;">interactions for bioremediation of metalliferous soils</p></div>
 +
        <div>
 +
            <span class="span1">
 +
          <p style="font-family: -webkit-body;font-size: 15px;">We hope to establish a sustainable, regulable and reusable project to solve the soil polluted by heavy metals. Phosphate-solubilizing bacteria have important functions. In our project, we used the mechanism of the alliance between microbe and plant, by manufacturing <i>Bacillus megaterium</i>, which is a kind of phosphate-solubilizing microorganism exists in the root system, forcing it enhance the plant remediation from two aspects, accumulating heavy metals and defend adversity stress. To achieve the goal of spatial specificity, we make most of the expression system which is regulated by root organic acid. We also used MBP (metal binding protein) and ACC deaminase to make accumulation of heavy metal in root system’s soil success. Finally, heavy metals will transfer to plant itself. A series of transformation we made in phosphate-solubilizing bacteria in our project will solve the weakness of hyper-accumulators, therefore, the remediation method will be put into use widely.
 +
<br/>&nbsp;&nbsp;&nbsp;&nbsp;</p></span>
 +
           
 +
            </span>
 +
        </div>
 +
    </div>
 +
    </div>
  
  
−
<div class="column half_size" >
+
 
−
<h5>Inspiration</h5>
+
<style type="text/css">
−
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
+
    .liuchengtu{
−
<ul>
+
    width: 60%
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
+
    margin: 0 auto
−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
    height:auto;
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
    }
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
    .liuchengtu1{
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
    width: 50%;
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
+
    margin-top: 100px
−
</ul>
+
    }
 +
    </style>
 +
<div align="center" class="liuchengtu">
 +
  <img class="liuchengtu1" src="https://static.igem.org/mediawiki/2017/e/e0/T--FAFU-CHINA--009.png">
 
</div>
 
</div>
  
−
<div class="column half_size" >
 
−
<h5> Uploading pictures and files </h5>
 
−
<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
−
When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
 
  
−
<a href="https://2017.igem.org/Special:Upload">
 
−
UPLOAD FILES
 
−
</a>
 
−
</p>
 
−
</div>
 
  
 +
 
 +
<style>
 +
.secondbg{
 +
background-color: #D0E090;
 +
margin:0 auto;
 +
padding:0;
 +
width:90%;
 +
    display：inline;
 +
}
 +
/* entire container, keeps perspective */
 +
.flip-container {
 +
perspective: 1000;
 +
}
 +
/* flip the pane when hovered */
 +
.flip-container:hover .flipper {
 +
transform: rotateY(180deg);
 +
}
  
 +
.flip-container, .front,.back{
 +
width: 320px;
 +
height: 490px;
 +
}
  
 +
/* flip speed goes here */
 +
.flipper {
 +
transition: 0.4s;
 +
transform-style: preserve-3d;
  
 +
position: relative;
 +
 +
}
 +
 +
/* hide back of pane during swap */
 +
.front, .back {
 +
-webkit-backface-visibility: hidden;
 +
    -moz-backface-visibility: hidden;
 +
 +
-ms-backface-visibility: hidden;
 +
-o-backface-visibility: hidden;
 +
 +
position: absolute;
 +
top: 0;
 +
left: 0;
 +
    border-radius:30px
 +
}
 +
 +
/* front pane, placed above back */
 +
.front {
 +
z-index: 1;
 +
}
 +
 +
/* back, initially hidden pane */
 +
.back {
 +
transform: rotateY(180deg);
 +
background-color: #D0E090;
 +
font-size: 20px
 +
}
 +
.front img{
 +
width:300px;
 +
}
 +
.back div {
 +
width:300px;
 +
height:450px;
 +
    margin-left: 10px
 +
/*background-color:#EBE6E6;*/
 +
}
 +
.toopp{
 +
padding:0;
 +
margin:0;
 +
}
 +
.picleft{
 +
float:right;
 +
margin:0;
 +
padding:0;
 +
width:40%;
 +
}
 +
.picleft img{
 +
width:100%;
 +
}
 +
.lleft{
 +
float:left;
 +
width:60%;
 +
}
 +
.lleft img{
 +
width:65%;
 +
float:left;
 +
margin:150px 0 0 100px;
 +
}
 +
.fgx{
 +
border:1px #D4CCCC solid;
 +
width:100%;
 +
opacity:0.5;
 +
}
 +
.fgx2{
 +
margin:10px 0;
 +
padding:0;
 +
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<div><p style="font-size:16px;font-family: -webkit-body;"><br/>Soil polluted by heavy metals represent an important environmental problem due to the toxic effects of metals, their accumulations throughout the food chain and the additional risk of groundwater contamination.
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Continued worldwide industrialization has caused extensive environmental and human health problems. A wide variety of chemicals, e.g., heavy metals, pesticides, chlorinated solvents, etc., have been detected in different natural resources such as soil, water, and air. Among the pollutants, the heavy metals are of concern to human health due to their cytotoxicity, mutagenicity, and carcinogenicity.
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          <div style="font-family: -webkit-body;"><br/><br/>Phytoextraction is based on the use of hyperaccumulator plants, which can tolerate and accumulate high concentration of metals. Ideal hyperaccumulators require the characteristics of deep rooted, rapid growth and a high amount of biomass. In fact, many hyperaccumulators are slow in growth, produce low bow biomass and cannot grow in metal-stress soil. </div>
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<div><p style="font-size:16px;font-family: -webkit-body;">&nbsp;&nbsp;&nbsp;Phosphorus is the second important key element after nitrogen as a mineral nutrient in terms of quantitative plant equirement. However, plants can use only a small amount of this P since 75–90% of added P is precipitated by metal–cation complexes, and rapidly becomes fixed in soils. In this regard, phosphate solubilizing microbes are considered as the most efficient and eco-friendly means for phytoremediation of metalliferous soils.
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          Although, several bacterial strains have been identified as PSB their performance under in situ conditions is not reliable. Therefore,we genetically engineered our chassis B. megaterium as a new strategy to accelerate phytoremediation process.</p>
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          <div><p style="font-family: -webkit-body;font-size: 18px" ><br/>In our project, the circuit uses a genetic ‘toggle switch’ architecture in which reciprocal repression by the LacI and TetR transcription factors form transcription states that are maintained by the circuit’s linked feedback loops. Inhibition of TetR expression by anhydrotetracy-cline (ATc), a compound that is not normally found in nature, is necessary for expression of LacI. Removal of ATc from the environment activates the expression of TetR, which leads to cell death.
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Latest revision as of 02:49, 2 November 2017

Overview

Synergism of Phosphate-solubilizing microoranisms-plant

interactions for bioremediation of metalliferous soils

We hope to establish a sustainable, regulable and reusable project to solve the soil polluted by heavy metals. Phosphate-solubilizing bacteria have important functions. In our project, we used the mechanism of the alliance between microbe and plant, by manufacturing Bacillus megaterium, which is a kind of phosphate-solubilizing microorganism exists in the root system, forcing it enhance the plant remediation from two aspects, accumulating heavy metals and defend adversity stress. To achieve the goal of spatial specificity, we make most of the expression system which is regulated by root organic acid. We also used MBP (metal binding protein) and ACC deaminase to make accumulation of heavy metal in root system’s soil success. Finally, heavy metals will transfer to plant itself. A series of transformation we made in phosphate-solubilizing bacteria in our project will solve the weakness of hyper-accumulators, therefore, the remediation method will be put into use widely.
    


Soil polluted by heavy metals represent an important environmental problem due to the toxic effects of metals, their accumulations throughout the food chain and the additional risk of groundwater contamination. Continued worldwide industrialization has caused extensive environmental and human health problems. A wide variety of chemicals, e.g., heavy metals, pesticides, chlorinated solvents, etc., have been detected in different natural resources such as soil, water, and air. Among the pollutants, the heavy metals are of concern to human health due to their cytotoxicity, mutagenicity, and carcinogenicity.
    



Phytoextraction is based on the use of hyperaccumulator plants, which can tolerate and accumulate high concentration of metals. Ideal hyperaccumulators require the characteristics of deep rooted, rapid growth and a high amount of biomass. In fact, many hyperaccumulators are slow in growth, produce low bow biomass and cannot grow in metal-stress soil.

   Phosphorus is the second important key element after nitrogen as a mineral nutrient in terms of quantitative plant equirement. However, plants can use only a small amount of this P since 75–90% of added P is precipitated by metal–cation complexes, and rapidly becomes fixed in soils. In this regard, phosphate solubilizing microbes are considered as the most efficient and eco-friendly means for phytoremediation of metalliferous soils. Although, several bacterial strains have been identified as PSB their performance under in situ conditions is not reliable. Therefore,we genetically engineered our chassis B. megaterium as a new strategy to accelerate phytoremediation process.


In our project, the circuit uses a genetic ‘toggle switch’ architecture in which reciprocal repression by the LacI and TetR transcription factors form transcription states that are maintained by the circuit’s linked feedback loops. Inhibition of TetR expression by anhydrotetracy-cline (ATc), a compound that is not normally found in nature, is necessary for expression of LacI. Removal of ATc from the environment activates the expression of TetR, which leads to cell death.