Difference between revisions of "Team:NYMU-Taipei/Notebook"

 
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{{NYMU-Taipei}}
 
{{NYMU-Taipei}}
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{{NYMU-Taipei-css}}
  
 
<html>
 
<html>
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<head>
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<head>
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  <title>Bootstrap Example</title>
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  <meta charset="utf-8">
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<style>
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  <meta name="viewport" content="width=device-width, initial-scale=1">
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body{
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  <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
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background-color:#f6ffb2;
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  <script src="https://ajax.googleapis.com/ajax/libs/jquery/3.2.1/jquery.min.js"></script>
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}
−
  <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
+
−
  <style>
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/* Content of Notebook */
−
  body {
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#Protocol{
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      position: relative;  
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width: 81%;
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  }
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padding: 2%;
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  #section1 {padding-top:50px;height:500px;color: #fff; background-color: #1E88E5;}
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display:block;
−
  #section2 {padding-top:50px;height:500px;color: #fff; background-color: #673ab7;}
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float:right;
−
  #section3 {padding-top:50px;height:500px;color: #fff; background-color: #ff9800;}
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background-color:#f6ffb2;
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  #section41 {padding-top:50px;height:500px;color: #fff; background-color: #00bcd4;}
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font-family:'Fresca',sans-serif;
−
  #section42 {padding-top:50px;height:500px;color: #fff; background-color: #009688;}
+
font-size:20px;
−
  </style>
+
}
−
</head>
+
−
<body >
+
#Timeline{
−
<div class="2017_igem_content_wrapper">
+
width: 81%;
 +
padding: 2%;
 +
display:block;
 +
float:right;
 +
background-color:#cee1ea;
 +
font-family:'Fresca',sans-serif;
 +
font-size:18px;
 +
}
 +
 +
#Labnote{
 +
width: 81%;
 +
padding:2%;
 +
display:block;
 +
float:right;
 +
background-color: #ffeaea;
 +
font-family:'Fresca',sans-serif;
 +
font-size:20px;
 +
padding-bottom:250px;
 +
}
 +
 +
#Protocol h1 {
 +
color: #205e1a;
 +
font-size: 40px;
 +
font-family: 'Acme', sans-serif;
 +
}
 +
 +
#Protocol h3 {
 +
color: #3dba32;
 +
font-size: 28px;
 +
font-family: 'Acme', sans-serif;
 +
padding-left:20px;
 +
}
 +
 +
#Protocol a {
 +
color:#205e1a;
 +
font-family: 'Acme', sans-serif;
 +
}
 +
 +
#Timeline h1{
 +
color: #1c3e4f;
 +
font-size: 40px;
 +
font-family: 'Acme', sans-serif;
 +
}
 +
 +
#Labnote h1{
 +
color: #722525;
 +
font-size: 40px;
 +
font-family: 'Acme', sans-serif;
 +
}
 +
 +
/* Show & hide */
 +
.expandable {
 +
/* overflow set to hidden to hide the expanded text */
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overflow: hidden;
 +
/* all style changes will ease-in-out for 1s */
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-moz-transition: all 1s ease-in-out;
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-ms-transition: all 1s ease-in-out;
 +
-o-transition: all 1s ease-in-out;
 +
-webkit-transition: all 1s ease-in-out;
 +
transition: all 1s;
 +
}
 +
 +
.panel {
 +
background-color: #fff;
 +
border: 1px solid transparent;
 +
border-radius: 4px;
 +
border-color: #ddd;
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-webkit-box-shadow: 0 1px 1px rgba(0, 0, 0, .05);
 +
box-shadow: 0 1px 1px rgba(0, 0, 0, .05);
 +
padding:15px;
 +
padding-top:20px;
 +
}
 +
 +
/* IF THE SCREEN IS LESS THAN 800PX */
 +
@media only screen and (max-width: 800px) {
 +
.igem_2017_footer_wrapper {width:100%; margin-left:0px;}
 +
#Protocol {width:96%; margin-left:50px;}
 +
#Timeline {width:96%; margin-left:50px;}
 +
#Labnote {width:96%; margin-left:50px;}
 +
}
 +
 +
</style>
 +
 +
<script>
 +
function toggleHeight1(e, maxHeight) {
 +
e = document.getElementById("s1"); // e = the gray div
 +
   
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if(e.style.height != '24px') {
 +
e.style.height = '24px'; // height of one line: 20px
 +
} else {
 +
e.style.height = maxHeight + 'px';
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}
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}
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function toggleHeight2(e, maxHeight) {
 +
e = document.getElementById("s2"); // e = the gray div
 +
   
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if(e.style.height != '24px') {
 +
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e.style.height = maxHeight + 'px';
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}
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function toggleHeight3(e, maxHeight) {
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e = document.getElementById("s3"); // e = the gray div
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if(e.style.height != '24px') {
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function toggleHeight4(e, maxHeight) {
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</script>
 +
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</head>
 +
 +
<body>
 +
 +
 +
 +
 +
<div id="Timeline" >
 +
                        <center>
 +
<h3></h3>
 +
<img src="https://static.igem.org/mediawiki/2017/f/f9/T--NYMU-Taipei--notebook.jpg"
 +
style="width:50%;border-radius:50%;border:5px solid #f6ffb2;">
 +
</center>
 +
<h3></h3>
 +
<h3></h3>
 +
<h1>Timeline</h1>
 +
<p></p>
 +
 +
<div class='panel'>
 +
<div id="s1" class="expandable" style='height: 24px;padding-top:5px;'>
 +
<a href="#!" onclick="toggleHeight1(this, 270); return false"
 +
style="font-family: 'Acme', sans-serif;font-size:28px;color: #479fcc;height: 20px;">
 +
June, 2017
 +
</a>
 +
<p>
 +
<br>
 +
<b>2017-06-19</b>&nbsp;&nbsp; prepare stock1 and stock5<br>
 +
<b>2017-06-20</b>&nbsp;&nbsp; (1) reprepare stock5<br>
 +
　　　　　(2) prepare BG11 medium<br>
 +
　　　　　(3) unfreeze <i>Synechocystis</i> sp. PCC6803 and start to cultivate in BG11<br>
 +
<b>2017-06-29</b>&nbsp;&nbsp; (1) prepare Pseudomonas aeruginosa growing medium<br>
 +
　　　　　(2) activate Pseudomonas aeruginosa and start to cultivate in Petri dish<br>
 +
　　　　　(3) subculture <i>Synechocystis</i> sp. PCC6803
 +
</p>
 +
 +
<center>
 +
<a href="#!" onclick="toggleHeight1(this, 270);" style='font-size:20px;color:#2c498c'>
 +
click to close
 +
</a>
 +
</center>
 +
</div>
 +
</div>
 +
 +
<div class='panel'>
 +
<div id="s2" class="expandable" style='height: 24px;padding-top:5px;'>
 +
<a href="#!" onclick="toggleHeight2(this, 1440); return false"
 +
style="font-family: 'Acme', sans-serif;font-size:28px;color: #479fcc;height: 20px;">
 +
July, 2017
 +
</a>
 +
<p>
 +
<br>
 +
<b>2017-07-02</b>&nbsp;&nbsp; extract <i>Pseudomonas aeruginosa</i> plasmid and freeze into refrigerator<br>
 +
<b>2017-07-03</b>&nbsp;&nbsp; proliferate <i>Pseudomonas aeruginosa</i> and freeze into refrigerator<br>
 +
<b>2017-07-04</b>&nbsp;&nbsp; (1) prepare BG11 medium<br>
 +
　　　　　(2) proliferate <i>Chlorella vulgaris</i> in Petri dish and centrifuge tube<br>
 +
<b>2017-07-05 to 07-10</b>&nbsp;&nbsp; cultivate <i>Synechocystis</i> sp. PCC6803 and <i>Chlorella vulgaris</i> in incubator<br>
 +
<b>2017-07-11</b>&nbsp;&nbsp;&nbsp;&nbsp; (1) extract <i>Synechocystis</i> sp. PCC6803 plasmid<br>
 +
　　　　　(2) prepare LB containing ampicillin and chloramphenicol and prepare Petri dish<br>
 +
<b>2017-07-12</b>&nbsp;&nbsp; transform BBa_K112806, BBa_K737007, BBa_K112000, BBa_J04450, BBa_K1356003, BBa_K1356004, and BBa_K356005 into competent cell<br>
 +
<b>2017-07-13</b>&nbsp;&nbsp;&nbsp; (1) proliferate 07-12 transformed E.coli<br>
 +
　　　　　(2) proliferate <i>Chlorella vulgaris</i><br>
 +
　　　　　(3) proliferate <i>Synechocystis</i> sp. PCC6803<br>
 +
　　　　　(4) activate <i>Synechococcus elongatus</i> PCC7942<br>
 +
<b>2017-07-14</b>&nbsp;&nbsp;&nbsp; (1)extract 07-13 proliferated E.coli plasmid<br>
 +
　　　　　(2) digestion: BBa_K112806, BBa_K737007, BBa_K112000, BBa_J04450, BBa_K1356003, BBa_K1356004, and BBa_K1356005<br>
 +
　　　　　　(check the correction of transformed E.coli)<br>
 +
<b>2017-07-17</b>&nbsp;&nbsp;&nbsp; (1) digestion again: BBa_K1356005, BBa_K112806, and BBa_K112000<br>
 +
　　　　　(2) retransform BBa_K112806 and BBa_K112000<br>
 +
<b>2017-07-18</b>&nbsp;&nbsp;&nbsp; (1) proliferate BBa_K112806 transformed E.coli (BBa_K112000 failed)<br>
 +
　　　　　(2) subculture <i>Synechococcus elongatus</i> PCC7942<br>
 +
　　　　　(3) cultivate <i>Synechocystis</i> sp. PCC6803 in BG11<br>
 +
<b>2017-07-19</b>&nbsp;&nbsp;&nbsp; (1) extract 07-18 BBa_K112806 transformed E.coli plasmid<br>
 +
　　　　　(2) digestion check<br>
 +
　　　　　(3) save BBa_K112806 transformed E.coli<br>
 +
　　　　　(4) save 07-18 <i>Synechocystis</i> sp. PCC6803<br>
 +
<b>2017-07-20</b>&nbsp;&nbsp; (1) activate Rhizobium etli in PY medium<br>
 +
　　　　　(2) dilute primer 001~008<br>
 +
　　　　　(3) run NrtA and terminator PCR (failed)<br>
 +
<b>2017-07-21 to 07-23</b>&nbsp;&nbsp; cultivate <i>Chlorella vulgaris</i> again for measuring growth curve<br>
 +
<b>2017-07-24</b>&nbsp;&nbsp; (1) prepare <i>Rhizobium etli</i> liquid culture<br>
 +
　　　　　(2) activate <i>Synechococcus elongatus</i> PCC7942<br>
 +
<b>2017-07-25</b>&nbsp;&nbsp; (1) extract <i>Synechocystis</i> sp. PCC6803 genomic DNA<br>
 +
　　　　　(2) run NrtA PCR<br>
 +
<b>2017-07-26</b>&nbsp;&nbsp; (1) extract <i>Rhizobium etli</i> genomic DNA<br>
 +
　　　　　(2) run NrtA and terminator PCR<br>
 +
　　　　　(3) run NrtA+ terminator fusion PCR (failed)<br>
 +
<b>2017-07-27</b>&nbsp;&nbsp; (1) run NrtA+ terminator fusion PCR again<br>
 +
　　　　　(2) purify the successful fusion product <br>
 +
　　　　　(3) digestion check<br>
 +
<b>2017-07-28</b>&nbsp;&nbsp; run the whole NrtA cloning procedure again, using KOD polymerase<br>
 +
　　　　　(1) run NrtA and terminator PCR<br>
 +
　　　　　(2) digestion check NrtA and terminator and purify the PCR product<br>
 +
　　　　　(3) run NrtA and terminator fusion PCR<br>
 +
　　　　　(4) digestion check and purify the fusion PCR product (failed)<br>
 +
<b>2017-07-29</b>&nbsp;&nbsp; (1) run NrtA and terminator fusion PCR again<br>
 +
　　　　　(2) digestion check and purify the fusion PCR product<br>
 +
　　　　　(3) ligation: backbone(psb1C3) and promoter, RBS, NrtA, and terminator<br>
 +
　　　　　(4) transformation in to E.coli<br>
 +
<b>2017-07-30</b>&nbsp;&nbsp; cultivate the transformed E.coli in liquid culture<br>
 +
<b>2017-07-31</b>&nbsp;&nbsp;&nbsp; (1) extract 07-30 transformed E.coli plasmid<br>
 +
　　　　　(2) restriction enzyme check (failed)<br>
 +
</p>
 +
 +
<center>
 +
<a href="#!" onclick="toggleHeight2(this,1440);" style='font-size:20px;color:#2c498c'>
 +
click to close
 +
</a>
 +
</center>
 +
</div>
 +
</div>
 +
 +
<div class='panel'>
 +
<div id="s3" class="expandable" style='height: 24px;padding-top:5px;'>
 +
<a href="#!" onclick="toggleHeight3(this, 2200); return false"
 +
style="font-family: 'Acme', sans-serif;font-size:28px;color: #479fcc;height: 20px;">
 +
August, 2017
 +
</a>
 +
<p>
 +
<br>
 +
<b>2017-08-04</b> &nbsp;&nbsp; Digest check: BBa_K112806<br>
 +
<b>2017-08-05</b> &nbsp;&nbsp; <i>Chlorella Vulgaris</i> growth curve measuring<br>
 +
<b>2017-08-06</b> &nbsp;&nbsp; PCR(Dream Taq): Sucide mechanism-BBa_K112806, K737007, J04450, K112000<br>
 +
<b>2017-08-07</b> &nbsp; (1) PCR(KOD plus): Sucide mechanism-BBa_K112806, K737007, J04450, K112000
 +
<br>　　　　　(2) NrtA- annealing, PNK, ligation, transformation<br>
 +
<b>2017-08-08</b> &nbsp;&nbsp; Suicide mechanism-Annealing, cloning, ligation, transformation<br>
 +
<b>2017-08-09</b> &nbsp;&nbsp; Extraction of <i>Synechoccocus</i> PCC7942 genomic DNA<br>
 +
<b>2017-08-10</b> &nbsp;&nbsp; (1) <i>Chlorella Vulgaris</i> growth curve measuring
 +
<br>　　　　　(2) Fusion PCR: NrtA + terminator
 +
<br>　　　　　(3) Digestion check: NrtA cut with BamH1
 +
<br>　　　　　(4) Subculture <i>S.elongatus</i> PCC7942<br>
 +
<b>2017-08-11</b> &nbsp;&nbsp;&nbsp; (1) Ligation: NrtA + terminator + promoter + RBS
 +
<br>　　　　　(2) Ligation: BBa_K737007+K112806, J04450+K112000 (Endolysin and Holin)
 +
<br>　　　　　(3) Transformation of Endolysin and Holin
 +
<br>　　　　　(4) Measure nitrate and nitrite concentration of <i>Chlorella Vulgaris</i>
 +
<br>　　　　　(5) OD value measurement of <i>Chlorella Vulgaris</i> and <i>S.elongatus</i> PCC7942<br>
 +
<b>2017-08-12</b> &nbsp;&nbsp; (1) Measure nitrate and nitrite concentration of <i>Chlorella Vulgaris</i>
 +
<br>　　　　　(2) OD value measurement of <i>Chlorella Vulgaris</i> and <i>S.elongatus</i> PCC7942<br>
 +
<b>2017-08-13</b> &nbsp;&nbsp; (1) Transformation: BBa_K274002 and K1152008 into E. coli
 +
<br>　　　　　(2) Measure nitrate and nitrite concentration of <i>Chlorella Vulgaris</i>
 +
<br>　　　　　(3) OD value measurement of <i>Chlorella Vulgaris</i> and <i>S.elongatus</i> PCC7942<br>
 +
<b>2017-08-14</b> &nbsp;&nbsp; (1) Colony PCR: K1152008
 +
<br>　　　　　(2) Suicide mechanism- PCR, annealing, cloning, ligation, transformation
 +
<br>　　　　　(3) NrtA-PCR, annealing, cloning, ligation, transformation
 +
<br>　　　　　(4) Liquid culture-K1152008<br>
 +
<b>2017-08-15</b> &nbsp;&nbsp; (1) Measure nitrate and nitrite concentration of <i>Chlorella Vulgaris</i>
 +
<br>　　　　　(2) OD value measurement of <i>Chlorella Vulgaris</i> and <i>S.elongatus</i> PCC7942
 +
<br>　　　　　(3) Suicide mechanism- final annealing, cloning, ligation, transformation
 +
<br>　　　　　(4) Plasmid extraction and RE check: k1152008<br>
 +
<b>2017-08-16</b> &nbsp;&nbsp; (1) Measure nitrate and nitrite concentration of <i>Chlorella Vulgaris</i>
 +
<br>　　　　　(2) OD value measurement of Chlorella Vulgaris and <i>S.elongatus</i> PCC7942
 +
<br>　　　　　(3) PCR(Dream Taq): PrbCL and indC<br>
 +
<b>2017-08-18</b> &nbsp;&nbsp; (1) Suicide mechanism- PCR, annealing, cloning, ligation, transformation
 +
<br>　　　　　(2) PCR(KOD plus): PrbCL and indC<br>
 +
<b>2017-08-19</b> &nbsp;&nbsp; (1) Suicide mechanism- PCR, annealing, cloning, ligation, transformation
 +
<br>　　　　　(2) Inoculate E. coli containing pBAD18
 +
<br>　　　　　(3) Fusion PCR: PrbCL+indC<br>
 +
<b>2017-08-22</b> &nbsp;&nbsp; (1) PCR: Lycopene and MelA
 +
<br>　　　　　(2) Fusion PCR: PrbCL+indC<br>
 +
<b>2017-08-24</b> &nbsp;&nbsp; (1) Liquid culture: BBa_K274002
 +
<br>　　　　　(2) Extract PCC7942 genomic DNA
 +
<br>　　　　　(3) Inoculate E. coli containing PBR322
 +
<br>　　　　　(4) Transformation: BBa_B0015 into E. coli<br>
 +
<b>2017-08-25</b> &nbsp;&nbsp; (1) Endolysin- PCR, annealing, cloning, ligation, transformation
 +
<br>　　　　　(2) Liquid culture: PBR322
 +
<br>　　　　　(3) Liquid culture: B0015<br>
 +
<b>2017-08-26</b> &nbsp;&nbsp; (1) Plasmid extraction: endolysin
 +
<br>　　　　　(2) Plasmid extraction: PBR322
 +
<br>　　　　　(3) Plasmid extraction: B0015<br>
 +
<b>2017-08-27</b> &nbsp;&nbsp; (1) PCR: Lycopene and MelA
 +
<br>　　　　　(2) Digestion check: PBR322
 +
<br>　　　　　(3) Digestion check: B0015
 +
<br>　　　　　(4) pigment backbone primer design
 +
<br>　　　　　(5) PCR (Dream Taq): CrtZ
 +
<br>　　　　　(6) Plasmid extraction: endolysin
 +
<br>　　　　　(7) Digestion check: endolysin<br>
 +
<b>2017-08-28</b> &nbsp;&nbsp; (1) Colony PCR: NrtA
 +
<br>　　　　　(2) Liquid culture: NrtA
 +
<br>　　　　　(3) Sequencing: endolysin
 +
<br>　　　　　(4) Fusion PCR: PrbcL+IndC
 +
<br>　　　　　(5) PCR(Dream taq): Holin(kit)
 +
<br>　　　　　(6) Check pBR322 concentration and transform pBR322<br>
 +
<b>2017-08-29</b> &nbsp;&nbsp; (1) Nile red oil measuring of <i>Chlorella Vulgaris</i> (~0901)
 +
<br>　　　　　(2) Plasmid extraction: NrtA
 +
<br>　　　　　(3) Holin construction
 +
<br>　　　　　(4) sequencing: NrtA<br>
 +
<b>2017-08-30</b> &nbsp;&nbsp; (1) PCR (Dream Taq): Ampicillin resistance, 5NSII, 3NSII, B0015, and PBR322 ori
 +
<br>　　　　　(2) Endolysin construct finish
 +
<br>　　　　　(3) Plasmid extraction: pBR322
 +
<br>　　　　　(4) Restriction enzyme check: pBR322
 +
<br>　　　　　(5) Backbone construct-3NSII, 5NSII,AMP, B0015, and ORI (PCR: dream taq)<br>
 +
<b>2017-08-31</b> &nbsp;&nbsp; (1) PCR (KOD plus): Ampicillin, 5NSII, 3NSII, B0015, and PBR322 ori
 +
<br>　　　　　(2) PCR (Dream Taq): PBR322 ori and B0015
 +
<br>　　　　　(3) pSB1C3-NrtA construct finish
 +
<br>　　　　　(4) Backbone construct-3NSII, 5NSII, AMP, B0015, and ORI (PCR: KOD)
 +
<br>　　　　　(5) Transform: BBa_K112000
 +
<br>　　　　　(6) LBA: glucose + CM (0.3%)
 +
</p>
 +
 +
<center>
 +
<a href="#!" onclick="toggleHeight3(this, 2200);" style='font-size:20px;color:#2c498c'>
 +
click to close
 +
</a>
 +
</center>
 +
</div>
 +
</div>
 +
 +
<div class='panel'>
 +
<div id="s4" class="expandable" style='height: 24px;padding-top:5px;'>
 +
<a href="#!" onclick="toggleHeight4(this, 1900); return false"
 +
style="font-family: 'Acme', sans-serif;font-size:28px;color: #479fcc;height: 20px;">
 +
September, 2017
 +
</a>
 +
<p>
 +
<br>
 +
<b>2017-09-01</b> &nbsp;&nbsp; (1)Fusion PCR: Ampicillin resistance, B0015
 +
<br>　　　　　(2) Three pieces PCR: 5NSII, 3NSII, and PBR322 ori<br>
 +
 
 +
<b>2017-09-02</b> &nbsp; (1) Ligation: Ampicillin resistance, B0015+ 5NSII, 3NSII, and PBR322 ori
 +
<br>　　　　　(2) Transformation: pPIGBACK (pigment plasmid backbone)
 +
<br>　　　　　(3) PCR (Dream Taq): CrtZ and PrbCL<br>
 +
 
 +
<b>2017-09-03</b> &nbsp; (1) Colony PCR: pPIGBACK
 +
<br>　　　　　(2) Liquid culture: pPIGBACK
 +
<br>　　　　　(3) PCR (KOD): CrtZ and PrbCL<br>
 +
 
 +
<b>2017-09-04</b> &nbsp; (1) Plasmid extraction: pPIGBACK
 +
<br>　　　　　(2) Digestion check: pPIGBACK
 +
<br>　　　　　(3) Fusion PCR: CrtZ+ PrbCL<br>
 +
 
 +
<b>2017-09-05</b> &nbsp; (1) pPIGBACK construct finish
 +
<br>　　　　　(2) Fusion PCR: PrbCL+IndC
 +
<br>　　　　　(3) Ligation: CrtZ+ PrbCL with pPIGBACK
 +
<br>　　　　　(4) Transformation: CrtZ construct<br>
 +
 
 +
<b>2017-09-06</b> &nbsp; (1) Colony pcr: CrtZ construct
 +
<br>　　　　　(2) Liquid culture: CrtZ
 +
<br>　　　　　(3) Lycopene primers design
 +
<br>　　　　　(4) IndC primers redesign<br>
 +
 
 +
<b>2017-09-07</b> &nbsp; (1) plasmid extraction: CrtZ-Ppigback
 +
<br>　　　　　(2) restriction enzyme check: CrtZ-pPIGBACK<br>
 +
 
 +
<b>2017-09-08</b> &nbsp; (1) transform CrtZ-Ppigback into PCC7942
 +
<br>　　　　　(2) nitrate and nitrite adsorption assay of Chlorella vulgaris (co-coculture with NrtA)
 +
<br>　　　　　(3) transform suicide mechanism construct (LBA+glucose 0.3%)<br>
 +
 
 +
<b>2017-09-09</b> &nbsp; (1) nitrate and nitrite adsorption assay of Chlorella vulgaris (co-coculture with NrtA)
 +
<br>　　　　　(2) fusion PCR: PrbcL+indC<br>
 +
 
 +
<b>2017-09-10</b> &nbsp;&nbsp; indC construct (~transformation)<br>
 +
 
 +
<b>2017-09-19</b> &nbsp;&nbsp; (1) Endolysin-Holin construct
 +
<br>　　　　　(2) Site-directed primers design<br>
 +
 
 +
<b>2017-09-20</b> &nbsp; (1) Liquid culture: BBa_J04450
 +
<br>　　　　　(2) Liquid culture: CrtZ-PCC7942 clones<br>
 +
 
 +
<b>2017-09-21</b> &nbsp;&nbsp; Plasmid extraction: J04450, CrtZ-PCC7942<br>
 +
 
 +
<b>2017-09-22</b> &nbsp;&nbsp; Site-directed PCR: 3NSII<br>
 +
 
 +
<b>2017-09-23</b> &nbsp;&nbsp; Site-directed-3NSII construct finish<br>
 +
 
 +
<b>2017-09-24</b> &nbsp; (1) plasmid extraction: Site-directed-3NSII
 +
<br>　　　　　(2) Site-directed PCR: 5NSII-1
 +
<br>　　　　　(3) Psb1c3-PrbcL construct finish<br>
 +
 
 +
<b>2017-09-25</b> &nbsp; (1) Psb1c3-NrtA construct finish
 +
<br>　　　　　(2) colony PCR: Psb1c3-PrbcL
 +
<br>　　　　　(3) Liquid culture: Psb1c3-PrbcL
 +
<br>　　　　　(4) IndC construct<br>
 +
 
 +
<b>2017-09-26</b> &nbsp; (1) Plasmid extraction and restriction enzyme check: Site-directed-5NSII-1
 +
<br>　　　　　(2) Liquid culture: Psb1c3-NrtA
 +
<br>　　　　　(3) Colony PCR: IndC
 +
<br>　　　　　(4) Psb1c3-3NSII construct finish
 +
<br>　　　　　(5) Site-directed PCR: 5NSII-2<br>
 +
 
 +
<b>2017-09-27</b> &nbsp; (1) Psb1c3-NrtA construct finish
 +
<br>　　　　　(2) Plasmid extraction and restriction enzyme check: IndC
 +
<br>　　　　　(3) Plasmid extraction and restriction enzyme check: IndC
 +
<br>　　　　　(4) Psb1c3-3NSII construct finish Plasmid extraction and RE check: Site-directed 5NSII-2
 +
<br>　　　　　(5) Site-directed PCR: AmpR<br>
 +
 
 +
<b>2017-09-28</b> &nbsp; (1) Plasmid extraction and RE check: Site-directed-AmpR
 +
<br>　　　　　(2) Plasmid extraction and RE check: Psb1c3-5NSII
 +
<br>　　　　　(3) Psb1c3-3NSII(SD) finish
 +
<br>　　　　　(4) Liquid culture: Psb1c3-PrbcL<br>
 +
 
 +
<b>2017-09-29</b> &nbsp; (1) Psb1c3-5NSII construct finish
 +
<br>　　　　　(2) Psb1c3-5NSII-ORI-3NSII construct finish
 +
<br>　　　　　(3) Colony PCR: Psb1c3-PrbcL- IndC
 +
<br>　　　　　(4) Colony PCR: Psb1c3-PrbcL- IndC
 +
<br>　　　　　(5) Psb1c3-PrbcL(SD) construct finish
 +
<br>　　　　　(6) Psb1c3-PrbcL-IndC construct finish
 +
<br>　　　　　(7) Site-directed: amp finish
 +
<br>　　　　　(8) transform CrtZ-pPIGBACK into PCC7942<br>
 +
 
 +
<b>2017-09-30</b> &nbsp; (1) Psb1c3-PrbcL(SD) finish
 +
<br>　　　　　(2) Liquid culture: CrtZ-pPIGBACK
 +
 
 +
</p>
 +
 +
<center>
 +
<a href="#!" onclick="toggleHeight4(this, 1900);" style='font-size:20px;color:#2c498c'>
 +
click to close
 +
</a>
 +
</center>
 +
</div>
 +
</div>
 +
 +
<div class='panel'>
 +
<div id="s5" class="expandable" style='height: 24px;padding-top:5px;'>
 +
<a href="#!" onclick="toggleHeight5(this, 2240); return false"
 +
style="font-family: 'Acme', sans-serif;font-size:28px;color: #479fcc;height: 20px;">
 +
October, 2017
 +
</a>
 +
<p>
 +
<br>
 +
<b>2017-10-01</b> &nbsp;&nbsp; (1) Colony PCR: 5NSII-Psb1c3
 +
<br>　　　　　(2) Liquid culture: 5NSII-Psb1c3
 +
<br>　　　　　(3) Colony PCR: 5NSII-ORI-3NSII-Psb1c3
 +
<br>　　　　　(4) Liquid culture: Site-directed mutated clones
 +
<br>　　　　　(5) Construct: Psb1c3-PrbcL-IndC
 +
<br>　　　　　(6) Liquid culture: NrtA-C33
 +
 
 +
<br><b>2017-10-02</b> &nbsp;&nbsp; (1) Finish all aite-directed mutagenesis
 +
<br>　　　　　(2) plasmid extraction: Psb1c3-5NSII, 5NSII-ORI-3NSII-Psb1c3
 +
<br>　　　　　(3) Digestion check: Psb1c3-5NSII, 5NSII-ORI-3NSII-Psb1c3
 +
<br>　　　　　(4) Colony PCR: Psb1c3-PrbcL-IndC
 +
<br>　　　　　(5) Liquid culture: Psb1c3-PrbcL-IndC
 +
<br>　　　　　(6) Liquid culture (French Press): NrtA-C33, competent cell
 +
 
 +
<br><b>2017-10-03</b> &nbsp;&nbsp; (1) French Press and dialysis
 +
<br>　　　　　(2) Sequencing: MelA
 +
<br>　　　　　(3) Construct Psb1c3-NrtA-Endolysin
 +
 
 +
<br><b>2017-10-04</b> &nbsp;&nbsp; (1) Construct pPIGBACK-J04450
 +
<br>　　　　　(2) Construct Psb1c3-B0015-AMP
 +
<br>　　　　　(3) Construct Psb1c3-PrbcL-CrtZ
 +
<br>　　　　　(4) check nitrate concentration
 +
<br>　　　　　(5) Colony PCR: NrtA-endolysin
 +
<br>　　　　　(6) Liquid culture: NrtA-endolysin
 +
 
 +
<br><b>2017-10-05</b> &nbsp;&nbsp; (1) Colony PCR: pPIGBACK-J04450
 +
<br>　　　　　(2) Liquid culture: pPIGBACK-J04450
 +
<br>　　　　　(3) Digestion check: NrtA-endolysin
 +
 
 +
<br><b>2017-10-06</b> &nbsp;&nbsp; (1) DNA extraction and digestion-pPIGBACK-CrtZ
 +
<br>　　　　　(2) Finish construct pPIGBACK-J04450
 +
<br>　　　　　(3) Colony PCR: Psb1c3-B0015-AMP
 +
<br>　　　　　(4) Liquid culture: Psb1c3-B0015-AMP
 +
 
 +
<br><b>2017-10-07</b> &nbsp;&nbsp; (1) Plasmid extraction: Psb1c3-B0015-AMP
 +
<br>　　　　　(2) Digest check: Psb1c3-B0015-AMP
 +
<br>　　　　　(3) Construct: Psb1c3-PrbcL-CrtZ
 +
 
 +
<br><b>2017-10-08</b> &nbsp;&nbsp; (1) Colony PCR: Psb1c3-PrbcL-CrtZ
 +
 
 +
<br><b>2017-10-09</b> &nbsp;&nbsp; (1) Liquid culture: competent cell for electroporation
 +
<br>　　　　　(2) Frech Press
 +
<br>　　　　　(3) Nitrate concentration assay
 +
 
 +
<br><b>2017-10-10</b> &nbsp;&nbsp; (1) Electroporation: competent cell
 +
 
 +
<br><b>2017-10-11</b> &nbsp;&nbsp; (1) Test electroporation: Psb1c3-J04450
 +
 
 +
<br><b>2017-10-13</b> &nbsp;&nbsp; (1) Construct: Psb1c3-IndC (electroporation)
 +
<br>　　　　　(2) Construct: pPIGBACK-IndC (electroporation)
 +
<br>　　　　　(3) Construct: pPIGBACK-PrbcL-CrtZ
 +
<br>　　　　　(4) Construct: Psb1c3-B0015-AMP
 +
<br>　　　　　(5) Starch functional test: pPIGBACK-PrbcL-CrtZ
 +
<br>　　　　　(6) Suicide mechanism functional test
 +
 
 +
<br><b>2017-10-14</b> &nbsp;&nbsp; (1) Colony PCR: pPIGBACK-IndC
 +
<br>　　　　　(2) Liquid culture: pPIGBACK-IndC
 +
<br>　　　　　(3) Colony PCR: pPIGBACK-PrbcL-CrtZ
 +
<br>　　　　　(4) <i>Chlorella Vulgaris</i> oil functional test: NrtA
 +
<br>　　　　　(5) Starch functional test: pPIGBACK-PrbcL-CrtZ
 +
<br>　　　　　(6) Suicide mechanism functional test
 +
 
 +
<br><b>2017-10-15</b> &nbsp;&nbsp; (1) Finish construct pPIGBACK-PrbcL-IndC, Psb1c3-PrbcL-IndC
 +
<br>　　　　　(2) Liquid culture: All parts
 +
<br>　　　　　(3) Starch functional test: pPIGBACK-PrbcL-CrtZ
 +
 
 +
<br><b>2017-10-16</b> &nbsp;&nbsp; (1) <i>Chlorella Vulgaris</i> oil functional test: NrtA
 +
<br>　　　　　(2) Plasmid extraction: all parts
 +
<br>　　　　　(3) Digestion check: all parts
 +
<br>　　　　　(4) Starch functional test: pPIGBACK-PrbcL-CrtZ
 +
 
 +
<br><b>2017-10-17</b> &nbsp;&nbsp; (1) NrtA add to device again
 +
<br>　　　　　(2) Starch functional test: pPIGBACK-PrbcL-CrtZ
 +
 
 +
<br><b>2017-10-18</b> &nbsp;&nbsp; (1) NrtA add to device again
 +
<br>　　　　　(2) Starch functional test: pPIGBACK-PrbcL-CrtZ
 +
 
 +
<br><b>2017-10-19</b> &nbsp;&nbsp; (1) NrtA add to device again
 +
<br>　　　　　(2) Starch functional test: pPIGBACK-PrbcL-CrtZ
 +
 
 +
<br><b>2017-10-20</b> &nbsp;&nbsp; (1) NrtA add to device again
 +
<br>　　　　　(2) Starch functional test: pPIGBACK-PrbcL-CrtZ
 +
 
 +
<br><b>2017-10-21</b> &nbsp;&nbsp; (1) NrtA add to device again
 +
<br>　　　　　(2) Starch functional test: pPIGBACK-PrbcL-CrtZ
 +
 
 +
<br><b>2017-10-22</b> &nbsp;&nbsp; (1) PCR: MelA, IndC
 +
<br>　　　　　(2) Ligation: Psb1c3-MelA, pPIGBACK-MelA, Psb1c3-IndC, pPIGBACK-IndC
 +
<br>　　　　　(3) Transformation: Psb1c3-MelA, pPIGBACK-MelA, Psb1c3-IndC, pPIGBACK-IndC
 +
 
 +
<br><b>2017-10-23</b> &nbsp;&nbsp; (1) PCR: MelA, IndC, Psb1c3-B0015-AMP
 +
<br>　　　　　(2) Ligation: Psb1c3-MelA, pPIGBACK-MelA, Psb1c3-IndC, pPIGBACK-IndC, Psb1c3-B0015-AMP
 +
<br>　　　　　(3) Liquid culture: Psb1c3-MelA, pPIGBACK-MelA, Psb1c3-IndC, pPIGBACK-IndC
  
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<div data-spy="scroll" data-target=".navbar" data-offset="50">
+
<br><b>2017-10-24</b> &nbsp;&nbsp; (1) PCR: PrbcL-MelA, Psb1c3-IndC, pPIGBACK-IndC
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<nav class="navbar navbar-inverse navbar-fixed-top">
+
<br>　　　　　(2) Ligation: Psb1c3-PrbcL-MelA, pPIGBACK-PrbcL-MelA
−
  <div class="container-fluid">
+
<br>　　　　　(3) Transformation: Psb1c3-PrbcL-MelA, pPIGBACK-PrbcL-MelA, Psb1c3-IndC, pPIGBACK-IndC, Psb1c3-B0015-AMP
−
    <div class="navbar-header">
+
<br>　　　　　(4) Liquid culture: Psb1c3-B0015-AMP
−
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+
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          <span class="icon-bar"></span>
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+
−
          <span class="icon-bar"></span>                       
+
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      </button>
+
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      <a class="navbar-brand" href="#">WebSiteName</a>
+
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    </div>
+
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    <div>
+
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      <div class="collapse navbar-collapse" id="myNavbar">
+
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        <ul class="nav navbar-nav">
+
−
          <li><a href="#section1">Section 1</a></li>
+
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          <li><a href="#section2">Section 2</a></li>
+
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          <li><a href="#section3">Section 3</a></li>
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          <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Section 4 <span class="caret"></span></a>
+
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            <ul class="dropdown-menu">
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              <li><a href="#section41">Section 4-1</a></li>
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              <li><a href="#section42">Section 4-2</a></li>
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</nav>   
+
  
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<div id="section1" class="container-fluid">
+
<br><b>2017-10-25</b> &nbsp;&nbsp; (1) Digestion check all parts
−
  <h1>Section 1</h1>
+
<br>　　　　　(2) Plasmid extraction: Psb1c3-B0015-AMP
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  <p>Try to scroll this section and look at the navigation bar while scrolling! Try to scroll this section and look at the navigation bar while scrolling!</p>
+
<br>　　　　　(3) Digestion check: Psb1c3-B0015-AMP
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  <p>Try to scroll this section and look at the navigation bar while scrolling! Try to scroll this section and look at the navigation bar while scrolling!</p>
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</div>
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<div id="section2" class="container-fluid">
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  <h1>Section 2</h1>
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  <p>Try to scroll this section and look at the navigation bar while scrolling! Try to scroll this section and look at the navigation bar while scrolling!</p>
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<div id="section3" class="container-fluid">
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  <h1>Section 3</h1>
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  <h1>Section 4 Submenu 2</h1>
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<div id="Labnote">
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<h1>Labnote</h1>
 +
<p></p>
 +
<a href="https://static.igem.org/mediawiki/2017/0/04/T--NYMU-Taipei--labnote.pdf" target="_blank">Download the file</a>
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Latest revision as of 16:14, 28 November 2017

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