Difference between revisions of "Team:Bielefeld-CeBiTec"

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{{Bielefeld-CeBiTec}}
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{{Team:Bielefeld-CeBiTec/Head}}
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{{Team:Bielefeld-CeBiTec/Header}}
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{{Team:Bielefeld-CeBiTec/CSS}}
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<html>
 
<html>
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<bod>
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<body>
  
−
<div class="column full_size" >
 
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<img src="http://placehold.it/2000x300/d3d3d3/f2f2f2">
 
−
</div>
 
  
  
 +
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 +
<div id="chart">
 +
<script src="https://d3js.org/d3.v4.min.js"></script>
 +
<script>
  
−
<div class="column full_size" >
+
        function rand (min, max) {return Math.floor(Math.random() * (max - min + 1)) + min;}
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<h1> Welcome to iGEM 2017! </h1>
+
−
<p>Your team has been approved and you are ready to start the iGEM season! </p>
+
−
</div>
+
  
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<div class="clear"></div>
+
        var svg = d3.select("#chart")
 +
    .append("svg")
 +
    .attr("width", 1580)
 +
      .attr("height", 500);
  
−
<div class="column half_size" >
+
        var ranobj = "";
−
<h5>Before you start: </h5>
+
        var rancol = "";
−
<p> Please read the following pages:</p>
+
−
<ul>
+
−
<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
+
−
<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
+
−
<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
+
−
</ul>
+
−
</div>
+
  
−
<div class="column half_size" >
 
−
<div class="highlight">
 
−
<h5> Styling your wiki </h5>
 
−
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 
−
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 
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</div>
 
−
</div>
 
  
−
<div class="column full_size" >
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        for(var i = 0; i < 200; i++){ranobj = ranobj + "" + rand(2, 2) + "";};
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<h5> Wiki template information </h5>
+
−
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
+
  
−
</div>
 
  
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<div class="column half_size" >
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            else if(x === 3){return "#8b8b8b";}
−
<h5> Editing your wiki </h5>
+
−
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
+
−
<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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    svg.append("svg:image")
 +
.attr("xlink:href", "https://static.igem.org/mediawiki/2017/4/4a/T--Bielefeld-CeBiTec--expand_monochrome_white_2.svg")
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$("svg").attr("width", 2000);
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      </script>
 +
  </div>
 +
 
 +
<div class="container start-page">
 +
 +
<!-- Screen 1 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/7/73/T--Bielefeld-CeBiTec--start-page-screen1.png">
 +
</div>
 +
<!-- Screen 2 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/c/c4/T--Bielefeld-CeBiTec--start-page-screen2.png">
 +
</div>
 +
<!-- Screen 3 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/a/ad/T--Bielefeld-CeBiTec--start-page-screen3.png">
 +
</div>
 +
<!-- Screen 4 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/1/13/T--Bielefeld-CeBiTec--start-page-screen4.png">
 +
</div>
 +
<!-- Screen 5 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/0/01/T--Bielefeld-CeBiTec--start-page-screen5.png">
 +
</div>
 +
<!-- Screen 6 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/3/3b/T--Bielefeld-CeBiTec--start-page-screen6.png">
 +
</div>
 +
<!-- Screen 7 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/8/81/T--Bielefeld-CeBiTec--start-page-screen7.png">
 +
</div>
 +
<!-- Screen 8 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/b/b4/T--Bielefeld-CeBiTec--start-page-screen8.png">
 +
</div>
 +
 +
<div class="contentbox" style="background: #efefef">
 +
<div class="content">
 +
<h2>Achievements</h2>
 +
<div class="article">
 +
The following list sums up all of our achievements and refers to pages of this wiki containing detailed information. Next to this we recorded the <b><a href="https://youtu.be/1f5vwcrvUac">original presentation</a></b> of our project in Boston at the Giant Jamboree, which sums up and explains the project in less than 20 minutes:
 +
</div>
 +
<iframe width="560" height="315" style="margin: auto; display: block; margin-top: 25px; margin-bottom: 25px;;" src="https://www.youtube.com/embed/1f5vwcrvUac" frameborder="0" gesture="media" allow="encrypted-media" allowfullscreen></iframe>
 +
<div lass="article">
 +
<h3>Awards</h3>
 +
<ul style="margin-left: 5%;">
 +
<li>Best Foundational Advance Project</li>
 +
<li>Best New Basic Part</li>
 +
</ul>
 +
<h3>Nominations</h3>
 +
<ul style="margin-left: 5%;">
 +
<li>Best Software</li>
 +
<li>Best Hardware</li>
 +
<li>Best New Composite Part</li>
 +
<li>Best Part Collection</li>
 +
</ul>
 +
</div>
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br>
 +
<b>We established two orthogonal methods for the detection of unnatural base pairs in a target sequence: an <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Software">Oxford Nanopore sequencing</a> application and an enzyme based detection method</b>
 +
</article>
 +
</div>
 
</div>
 
</div>
  
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b>Development of a <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Software">software</a> suite for these orthogonal methods </b>
 +
</article>
 +
</div>
 +
</div>
  
−
<div class="column half_size" >
+
<div class="contentline">
−
<h5>Tips</h5>
+
<div class="third" style="width: 15%; margin-left: 5%;">
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
<div class="figure large">
−
<ul>
+
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
<p class="figure subtitle"></p>
−
<li>Be clear about what you are doing and how you plan to do this.</li>
+
</div>
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
</div>
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
<div class="third double">
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
<article><br>
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
+
<b>Integration and characterization of the <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/unnatural_base_pair/uptake_and_biosynthesis">nucleotide transporter PtNTT2</a> from <i>P.tricornutum</i> in <i>E.coli</i> for the uptake of unnatural nucleoside triphosphates</b>
−
<li>Have lots of fun! </li>
+
</article>
−
</ul>  
+
</div>
 
</div>
 
</div>
  
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b>Confirmation that certain Taq DNA polymerases can efficiently <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/unnatural_base_pair/preservation_system">incorporate unnatural nucleotides</a> </b>
 +
</article>
 +
</div>
 +
</div>
  
−
<div class="column half_size" >
+
<div class="contentline">
−
<h5>Inspiration</h5>
+
<div class="third" style="width: 15%; margin-left: 5%;">
−
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
+
<div class="figure large">
−
<ul>
+
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
+
<p class="figure subtitle"></p>
−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
</div>
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
</div>
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
<div class="third double">
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
<article><br><br>
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
+
<b>Construction of a <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox">toolbox</a> consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids</b>
−
</ul>
+
</article>
 +
</div>
 
</div>
 
</div>
  
−
<div class="column half_size" >
+
<div class="contentline">
−
<h5> Uploading pictures and files </h5>
+
<div class="third" style="width: 15%; margin-left: 5%;">
−
<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
+
<div class="figure large">
−
When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
+
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b><a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox/labeling">Colocalization</a> of the RuBisCo and and subcellular compartment (carboxysome) using a fluorescent amino acid</b>
 +
</article>
 +
</div>
 +
</div>         
 +
           
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b>Development of a <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox/photoswitching">photoswitchable lycopene pathway</a></b>
 +
</article>
 +
</div>
 +
</div>
  
−
<a href="https://2017.igem.org/Special:Upload">
+
<div class="contentline">
−
UPLOAD FILES
+
<div class="third" style="width: 15%; margin-left: 5%;">
−
</a>
+
<div class="figure large">
−
</p>
+
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br>
 +
<b>Design, <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox/fusing">chemical synthesis</a> and proof of functionality of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine</b>
 +
</article>
 +
</div>
 
</div>
 
</div>
  
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
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<b><a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Model">Modeling</a> more than ten new aaRS sequences</b>
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<b><a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/translational_system/library_and_selection">Library development</a> with several hundred thousand sequences for selecting aminoacyl-tRNA synthetases</b>
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<b>Construction of positive and negative <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/translational_system/library_and_selection">selection plasmids</a> for the evolution of new synthetases for non-canonical amino acids</b>
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<b><a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Improve">Improvement</a> of an aminoacyl-tRNA synthetase test-system by introducing a FRET-system and development of a ranking system</b>
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<b>Construction of an <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Hardware">LED panel</a> for irradiating 96-well microtiter plates, which can be used to manipulate non-canonical amino acids and for other applications  </b>
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<b>Development of an <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Software">Android</a> App to control the LED panel with your smartphone via Bluetooth</b>
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<b>Writing a <a target="_blank" href="https://static.igem.org/mediawiki/2017/1/1e/T--Bielefeld-CeBiTec--DKE_Biosafety_Report.pdf">biosafety report</a> entitled “Auxotrophy to Xeno-DNA: A Comprehensive Exploration of Combinatorial Mechanisms for a High-Fidelity Biosafety System” </b>
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<b>Writing the <a target="_blank" href="https://static.igem.org/mediawiki/2017/1/18/T--Bielefeld-CeBiTec--CMZ-ChImp.pdf">ChImp Report</a> on “Chances and Implications of an Expanded Genetic Code”</b>
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Latest revision as of 15:18, 16 December 2017

Achievements

The following list sums up all of our achievements and refers to pages of this wiki containing detailed information. Next to this we recorded the original presentation of our project in Boston at the Giant Jamboree, which sums up and explains the project in less than 20 minutes:

Awards

  • Best Foundational Advance Project
  • Best New Basic Part

Nominations

  • Best Software
  • Best Hardware
  • Best New Composite Part
  • Best Part Collection


We established two orthogonal methods for the detection of unnatural base pairs in a target sequence: an Oxford Nanopore sequencing application and an enzyme based detection method



Development of a software suite for these orthogonal methods


Integration and characterization of the nucleotide transporter PtNTT2 from P.tricornutum in E.coli for the uptake of unnatural nucleoside triphosphates



Construction of a toolbox consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids



Colocalization of the RuBisCo and and subcellular compartment (carboxysome) using a fluorescent amino acid


Design, chemical synthesis and proof of functionality of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine



Modeling more than ten new aaRS sequences



Library development with several hundred thousand sequences for selecting aminoacyl-tRNA synthetases


Construction of positive and negative selection plasmids for the evolution of new synthetases for non-canonical amino acids



Improvement of an aminoacyl-tRNA synthetase test-system by introducing a FRET-system and development of a ranking system



Construction of an LED panel for irradiating 96-well microtiter plates, which can be used to manipulate non-canonical amino acids and for other applications



Development of an Android App to control the LED panel with your smartphone via Bluetooth


Writing a biosafety report entitled “Auxotrophy to Xeno-DNA: A Comprehensive Exploration of Combinatorial Mechanisms for a High-Fidelity Biosafety System”



Writing the ChImp Report on “Chances and Implications of an Expanded Genetic Code”