Difference between revisions of "Team:NYMU-Taipei/Model"
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<img src='https://static.igem.org/mediawiki/2017/0/04/T--NYMU-Taipei--model_growth_curve.gif' | <img src='https://static.igem.org/mediawiki/2017/0/04/T--NYMU-Taipei--model_growth_curve.gif' | ||
alt='Growth curve of Chlorella vulgaris' | alt='Growth curve of Chlorella vulgaris' | ||
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<p style='font-size:20px'>fig.1 Growth curve of <i>Chlorella vulgaris</i></p> | <p style='font-size:20px'>fig.1 Growth curve of <i>Chlorella vulgaris</i></p> | ||
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<img src='https://static.igem.org/mediawiki/2017/e/e5/T--NYMU-Taipei--model_growth_rate.gif' | <img src='https://static.igem.org/mediawiki/2017/e/e5/T--NYMU-Taipei--model_growth_rate.gif' | ||
alt='Growth rate of Chlorella vulgaris' | alt='Growth rate of Chlorella vulgaris' | ||
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<p style='font-size:20px'>fig.2 Growth rate of <i>Chlorella vulgaris</i></p> | <p style='font-size:20px'>fig.2 Growth rate of <i>Chlorella vulgaris</i></p> | ||
</center> | </center> | ||
Revision as of 07:15, 15 August 2017
Modeling
Growth curve of Chlorella vulgaris
The timing of adding engineering E.coli or purified protein to Chlorella vulgaris culture is critical for our project. Through the initial, final biomass concentration data, the instantaneous rate of a reference time and other lab environment datas, we simulate the change in biomass concentration throughout the culture cycle. The status information in the culture medium at each point is then obtained through the other calculus to obtain the best timing point and the corresponding state.
ln(Xt/X0)/t=A+Bexp(-C(t-M))=μ(specific growth rate)
X: biomass concentration(g/l)
t: time(hr)
A: the asymptotic of ln Xt/Xo as t decrese indefinitely
B: the asymptotic of ln Xt/Xo as t increase indefinitely
C: the relative growth rate at time M
If there are some key condition changed, we use following equation to correct the model. (we culture the microalgae in incubator ,it surrounding temperature is stable.)
μ=KI/(Ki+I+I^2/Kii);
=μmS/(Ks+S+S^2/Kss);
μm=μm*/(Kn+N+N^2/Knn);
K: constant.
Ki: saturation constant of light intensity
Kii: inhibition constant of light intensity
Ks: inhibition constant of substrate
Kss: saturation constant of substrate
μm: maximum specific growth rate
Kn: inhibition constant of nitrogen
Knn: saturation constant of nitrogen
μm*: constant
fig.1 Growth curve of Chlorella vulgaris
fig.2 Growth rate of Chlorella vulgaris
Oil accumulation & Nirogen source consumption
Simulating common system of oil accumulation and nitrogen source consumption, not only get the reference of state before the improvement as well as the stage information, but also as a basic equation after some parameters or organisms join into the system.
dP/dt=*dX/dt+*X;
dN/dt=-V*X;
V=((qM-Q)/(qM-q))*((Vm*N)/(N+Vh));
Q=(X0*Q0+N0-N)/X;
X: biomass concentration(g/l)
P: lipid
N: nitrogen
X: biomass
α: the instantaneous yield coefficient of product formation due to cell growth
β: the specific formation rate of product
q: Minimum N quota
qM: Maximum N quota
Q: N quota
Vm: Maximum uptake rate of nitrogen
Vh: Half-saturation coefficient
fig.3 Oil accumulation and nirogen source consumption at normal situation
