Difference between revisions of "Team:NYMU-Taipei/Notebook"

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October, 2017
 
October, 2017
 
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<p>
 
<p>
<b>2017-06-19</b>&nbsp;&nbsp; prepare stock1 and stock5<br>
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<br>
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<b>2017-10-01</b> &nbsp;&nbsp; (1) Colony PCR: 5NSII-Psb1c3
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<br>     (2) Liquid culture: 5NSII-Psb1c3
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<br>     (3) Colony PCR: 5NSII-ORI-3NSII-Psb1c3
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<br>     (4) Liquid culture: Site-directed mutated clones
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<br>     (5) Construct: Psb1c3-PrbcL-IndC
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<br>     (6) Liquid culture: NrtA-C33
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<br><b>2017-10-02</b> &nbsp;&nbsp; (1) Finish all aite-directed mutagenesis
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<br>     (2) plasmid extraction: Psb1c3-5NSII, 5NSII-ORI-3NSII-Psb1c3
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<br>     (3) Digestion check: Psb1c3-5NSII, 5NSII-ORI-3NSII-Psb1c3
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<br>     (4) Colony PCR: Psb1c3-PrbcL-IndC
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<br>     (5) Liquid culture: Psb1c3-PrbcL-IndC
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<br>     (6) Liquid culture (French Press): NrtA-C33, competent cell
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<br><b>2017-10-03</b> &nbsp;&nbsp; (1) French Press and dialysis
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<br>     (2) Sequencing: MelA
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<br>     (3) Construct Psb1c3-NrtA-Endolysin
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<br><b>2017-10-04</b> &nbsp;&nbsp; (1) Construct pPIGBACK-J04450
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<br>     (2) Construct Psb1c3-B0015-AMP
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<br>     (3) Construct Psb1c3-PrbcL-CrtZ
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<br>     (4) check nitrate concentration
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<br>     (5) Colony PCR: NrtA-endolysin
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<br>     (6) Liquid culture: NrtA-endolysin
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<br><b>2017-10-05</b> &nbsp;&nbsp; (1) Colony PCR: pPIGBACK-J04450
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<br>     (2) Liquid culture: pPIGBACK-J04450
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<br>     (3) Digestion check: NrtA-endolysin
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<br><b>2017-10-06</b> &nbsp;&nbsp; (1) DNA extraction and digestion-pPIGBACK-CrtZ
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<br>     (2) Finish construct pPIGBACK-J04450
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<br>     (3) Colony PCR: Psb1c3-B0015-AMP
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<br>     (4) Liquid culture: Psb1c3-B0015-AMP
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 +
<br><b>2017-10-07</b> &nbsp;&nbsp; (1) Plasmid extraction: Psb1c3-B0015-AMP
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<br>     (2) Digest check: Psb1c3-B0015-AMP
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<br>     (3) Construct: Psb1c3-PrbcL-CrtZ
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<br><b>2017-10-08</b> &nbsp;&nbsp; (1) Colony PCR: Psb1c3-PrbcL-CrtZ
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<br><b>2017-10-09</b> &nbsp;&nbsp; (1) Liquid culture: competent cell for electroporation
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<br>     (2) Frech Press
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<br>     (3) Nitrate concentration assay
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<br><b>2017-10-10</b> &nbsp;&nbsp; (1) Electroporation: competent cell
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<br><b>2017-10-11</b> &nbsp;&nbsp; (1) Test electroporation: Psb1c3-J04450
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<br><b>2017-10-13</b> &nbsp;&nbsp; (1) Construct: Psb1c3-IndC (electroporation)
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<br>     (2) Construct: pPIGBACK-IndC (electroporation)
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<br>     (3) Construct: pPIGBACK-PrbcL-CrtZ
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<br>     (4) Construct: Psb1c3-B0015-AMP
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<br>     (5) Starch functional test: pPIGBACK-PrbcL-CrtZ
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<br>     (6) Suicide mechanism functional test
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<br><b>2017-10-14</b> &nbsp;&nbsp; (1) Colony PCR: pPIGBACK-IndC
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<br>     (2) Liquid culture: pPIGBACK-IndC
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<br>     (3) Colony PCR: pPIGBACK-PrbcL-CrtZ
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<br>     (4) <i>Chlorella Vulgaris</i> oil functional test: NrtA
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<br>     (5) Starch functional test: pPIGBACK-PrbcL-CrtZ
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<br>     (6) Suicide mechanism functional test
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 +
<br><b>2017-10-15</b> &nbsp;&nbsp; (1) Finish construct pPIGBACK-PrbcL-IndC, Psb1c3-PrbcL-IndC
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<br>     (2) Liquid culture: All parts
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<br>     (3) Starch functional test: pPIGBACK-PrbcL-CrtZ
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 +
<br><b>2017-10-16</b> &nbsp;&nbsp; (1) <i>Chlorella Vulgaris</i> oil functional test: NrtA
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<br>     (2) Plasmid extraction: all parts
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<br>     (3) Digestion check: all parts
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<br>     (4) Starch functional test: pPIGBACK-PrbcL-CrtZ
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 +
<br><b>2017-10-17</b> &nbsp;&nbsp; (1) NrtA add to device again
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<br>     (2) Starch functional test: pPIGBACK-PrbcL-CrtZ
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 +
<br><b>2017-10-18</b> &nbsp;&nbsp; (1) NrtA add to device again
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<br>     (2) Starch functional test: pPIGBACK-PrbcL-CrtZ
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 +
<br><b>2017-10-19</b> &nbsp;&nbsp; (1) NrtA add to device again
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<br>     (2) Starch functional test: pPIGBACK-PrbcL-CrtZ
 +
 
 +
<br><b>2017-10-20</b> &nbsp;&nbsp; (1) NrtA add to device again
 +
<br>     (2) Starch functional test: pPIGBACK-PrbcL-CrtZ
 +
 
 +
<br><b>2017-10-21</b> &nbsp;&nbsp; (1) NrtA add to device again
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<br>     (2) Starch functional test: pPIGBACK-PrbcL-CrtZ
 +
 
 +
<br><b>2017-10-22</b> &nbsp;&nbsp; (1) PCR: MelA, IndC
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<br>     (2) Ligation: Psb1c3-MelA, pPIGBACK-MelA, Psb1c3-IndC, pPIGBACK-IndC
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<br>     (3) Transformation: Psb1c3-MelA, pPIGBACK-MelA, Psb1c3-IndC, pPIGBACK-IndC
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 +
<br><b>2017-10-23</b> &nbsp;&nbsp; (1) PCR: MelA, IndC, Psb1c3-B0015-AMP
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<br>     (2) Ligation: Psb1c3-MelA, pPIGBACK-MelA, Psb1c3-IndC, pPIGBACK-IndC, Psb1c3-B0015-AMP
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<br>     (3) Liquid culture: Psb1c3-MelA, pPIGBACK-MelA, Psb1c3-IndC, pPIGBACK-IndC
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 +
<br><b>2017-10-24</b> &nbsp;&nbsp; (1) PCR: PrbcL-MelA, Psb1c3-IndC, pPIGBACK-IndC
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<br>     (2) Ligation: Psb1c3-PrbcL-MelA, pPIGBACK-PrbcL-MelA
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<br>     (3) Transformation: Psb1c3-PrbcL-MelA, pPIGBACK-PrbcL-MelA, Psb1c3-IndC, pPIGBACK-IndC, Psb1c3-B0015-AMP
 +
<br>     (4) Liquid culture: Psb1c3-B0015-AMP
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 +
<br><b>2017-10-25</b> &nbsp;&nbsp; (1) Digestion check all parts
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<br>     (2) Plasmid extraction: Psb1c3-B0015-AMP
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<br>     (3) Digestion check: Psb1c3-B0015-AMP
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Revision as of 13:37, 26 October 2017

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