Line 496: | Line 496: | ||
<div class='panel'> | <div class='panel'> | ||
<div id="s5" class="expandable" style='height: 24px;padding-top:5px;'> | <div id="s5" class="expandable" style='height: 24px;padding-top:5px;'> | ||
− | <a href="#!" onclick="toggleHeight5(this, | + | <a href="#!" onclick="toggleHeight5(this, 1240); return false" |
style="font-family: 'Acme', sans-serif;font-size:28px;color: #479fcc;height: 20px;"> | style="font-family: 'Acme', sans-serif;font-size:28px;color: #479fcc;height: 20px;"> | ||
October, 2017 | October, 2017 | ||
</a> | </a> | ||
<p> | <p> | ||
− | <b>2017-06-19</b> | + | <br> |
+ | <b>2017-10-01</b> (1) Colony PCR: 5NSII-Psb1c3 | ||
+ | <br> (2) Liquid culture: 5NSII-Psb1c3 | ||
+ | <br> (3) Colony PCR: 5NSII-ORI-3NSII-Psb1c3 | ||
+ | <br> (4) Liquid culture: Site-directed mutated clones | ||
+ | <br> (5) Construct: Psb1c3-PrbcL-IndC | ||
+ | <br> (6) Liquid culture: NrtA-C33 | ||
+ | |||
+ | <br><b>2017-10-02</b> (1) Finish all aite-directed mutagenesis | ||
+ | <br> (2) plasmid extraction: Psb1c3-5NSII, 5NSII-ORI-3NSII-Psb1c3 | ||
+ | <br> (3) Digestion check: Psb1c3-5NSII, 5NSII-ORI-3NSII-Psb1c3 | ||
+ | <br> (4) Colony PCR: Psb1c3-PrbcL-IndC | ||
+ | <br> (5) Liquid culture: Psb1c3-PrbcL-IndC | ||
+ | <br> (6) Liquid culture (French Press): NrtA-C33, competent cell | ||
+ | |||
+ | <br><b>2017-10-03</b> (1) French Press and dialysis | ||
+ | <br> (2) Sequencing: MelA | ||
+ | <br> (3) Construct Psb1c3-NrtA-Endolysin | ||
+ | |||
+ | <br><b>2017-10-04</b> (1) Construct pPIGBACK-J04450 | ||
+ | <br> (2) Construct Psb1c3-B0015-AMP | ||
+ | <br> (3) Construct Psb1c3-PrbcL-CrtZ | ||
+ | <br> (4) check nitrate concentration | ||
+ | <br> (5) Colony PCR: NrtA-endolysin | ||
+ | <br> (6) Liquid culture: NrtA-endolysin | ||
+ | |||
+ | <br><b>2017-10-05</b> (1) Colony PCR: pPIGBACK-J04450 | ||
+ | <br> (2) Liquid culture: pPIGBACK-J04450 | ||
+ | <br> (3) Digestion check: NrtA-endolysin | ||
+ | |||
+ | <br><b>2017-10-06</b> (1) DNA extraction and digestion-pPIGBACK-CrtZ | ||
+ | <br> (2) Finish construct pPIGBACK-J04450 | ||
+ | <br> (3) Colony PCR: Psb1c3-B0015-AMP | ||
+ | <br> (4) Liquid culture: Psb1c3-B0015-AMP | ||
+ | |||
+ | <br><b>2017-10-07</b> (1) Plasmid extraction: Psb1c3-B0015-AMP | ||
+ | <br> (2) Digest check: Psb1c3-B0015-AMP | ||
+ | <br> (3) Construct: Psb1c3-PrbcL-CrtZ | ||
+ | |||
+ | <br><b>2017-10-08</b> (1) Colony PCR: Psb1c3-PrbcL-CrtZ | ||
+ | |||
+ | <br><b>2017-10-09</b> (1) Liquid culture: competent cell for electroporation | ||
+ | <br> (2) Frech Press | ||
+ | <br> (3) Nitrate concentration assay | ||
+ | |||
+ | <br><b>2017-10-10</b> (1) Electroporation: competent cell | ||
+ | |||
+ | <br><b>2017-10-11</b> (1) Test electroporation: Psb1c3-J04450 | ||
+ | |||
+ | <br><b>2017-10-13</b> (1) Construct: Psb1c3-IndC (electroporation) | ||
+ | <br> (2) Construct: pPIGBACK-IndC (electroporation) | ||
+ | <br> (3) Construct: pPIGBACK-PrbcL-CrtZ | ||
+ | <br> (4) Construct: Psb1c3-B0015-AMP | ||
+ | <br> (5) Starch functional test: pPIGBACK-PrbcL-CrtZ | ||
+ | <br> (6) Suicide mechanism functional test | ||
+ | |||
+ | <br><b>2017-10-14</b> (1) Colony PCR: pPIGBACK-IndC | ||
+ | <br> (2) Liquid culture: pPIGBACK-IndC | ||
+ | <br> (3) Colony PCR: pPIGBACK-PrbcL-CrtZ | ||
+ | <br> (4) <i>Chlorella Vulgaris</i> oil functional test: NrtA | ||
+ | <br> (5) Starch functional test: pPIGBACK-PrbcL-CrtZ | ||
+ | <br> (6) Suicide mechanism functional test | ||
+ | |||
+ | <br><b>2017-10-15</b> (1) Finish construct pPIGBACK-PrbcL-IndC, Psb1c3-PrbcL-IndC | ||
+ | <br> (2) Liquid culture: All parts | ||
+ | <br> (3) Starch functional test: pPIGBACK-PrbcL-CrtZ | ||
+ | |||
+ | <br><b>2017-10-16</b> (1) <i>Chlorella Vulgaris</i> oil functional test: NrtA | ||
+ | <br> (2) Plasmid extraction: all parts | ||
+ | <br> (3) Digestion check: all parts | ||
+ | <br> (4) Starch functional test: pPIGBACK-PrbcL-CrtZ | ||
+ | |||
+ | <br><b>2017-10-17</b> (1) NrtA add to device again | ||
+ | <br> (2) Starch functional test: pPIGBACK-PrbcL-CrtZ | ||
+ | |||
+ | <br><b>2017-10-18</b> (1) NrtA add to device again | ||
+ | <br> (2) Starch functional test: pPIGBACK-PrbcL-CrtZ | ||
+ | |||
+ | <br><b>2017-10-19</b> (1) NrtA add to device again | ||
+ | <br> (2) Starch functional test: pPIGBACK-PrbcL-CrtZ | ||
+ | |||
+ | <br><b>2017-10-20</b> (1) NrtA add to device again | ||
+ | <br> (2) Starch functional test: pPIGBACK-PrbcL-CrtZ | ||
+ | |||
+ | <br><b>2017-10-21</b> (1) NrtA add to device again | ||
+ | <br> (2) Starch functional test: pPIGBACK-PrbcL-CrtZ | ||
+ | |||
+ | <br><b>2017-10-22</b> (1) PCR: MelA, IndC | ||
+ | <br> (2) Ligation: Psb1c3-MelA, pPIGBACK-MelA, Psb1c3-IndC, pPIGBACK-IndC | ||
+ | <br> (3) Transformation: Psb1c3-MelA, pPIGBACK-MelA, Psb1c3-IndC, pPIGBACK-IndC | ||
+ | |||
+ | <br><b>2017-10-23</b> (1) PCR: MelA, IndC, Psb1c3-B0015-AMP | ||
+ | <br> (2) Ligation: Psb1c3-MelA, pPIGBACK-MelA, Psb1c3-IndC, pPIGBACK-IndC, Psb1c3-B0015-AMP | ||
+ | <br> (3) Liquid culture: Psb1c3-MelA, pPIGBACK-MelA, Psb1c3-IndC, pPIGBACK-IndC | ||
+ | |||
+ | <br><b>2017-10-24</b> (1) PCR: PrbcL-MelA, Psb1c3-IndC, pPIGBACK-IndC | ||
+ | <br> (2) Ligation: Psb1c3-PrbcL-MelA, pPIGBACK-PrbcL-MelA | ||
+ | <br> (3) Transformation: Psb1c3-PrbcL-MelA, pPIGBACK-PrbcL-MelA, Psb1c3-IndC, pPIGBACK-IndC, Psb1c3-B0015-AMP | ||
+ | <br> (4) Liquid culture: Psb1c3-B0015-AMP | ||
+ | |||
+ | <br><b>2017-10-25</b> (1) Digestion check all parts | ||
+ | <br> (2) Plasmid extraction: Psb1c3-B0015-AMP | ||
+ | <br> (3) Digestion check: Psb1c3-B0015-AMP | ||
+ | |||
</p> | </p> | ||
<center> | <center> | ||
− | <a href="#!" onclick="toggleHeight5(this, | + | <a href="#!" onclick="toggleHeight5(this, 1240);" style='font-size:20px;color:#2c498c'> |
click to close | click to close | ||
</a> | </a> |
Revision as of 13:37, 26 October 2017