Difference between revisions of "Team:GZHS-United/Safety"

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{{GZHS-United}}
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<title>satety</title>
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<link href="https://2017.igem.org/Team:GZHS-United/CSS/homestyle?action=raw&ctype=text/css" rel="stylesheet" type="text/css" media="all" />
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<link href="https://2017.igem.org/Team:GZHS-United/CSS/bootstrapmin?action=raw&ctype=text/css" rel="stylesheet" type="text/css"/>
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<script type="text/javascript" src="https://2017.igem.org/Team:GZHS-United/JS/jquerymin?action=raw&ctype=text/javascript"></script>
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#content { padding:0px; width:100%; margin-top:-12px; margin-left:0px;}
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    font-family: 'Roboto Slab', serif;
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</head>
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<body>
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<div class="header-banner" id="head">
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<div class="header-bottom">
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<div class="fixed-header">
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<div class="container">
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<div class="top-menu">
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<span class="menu"><img src="https://static.igem.org/mediawiki/2017/8/83/T--GZHS-United--home_nav.png" alt=""/> </span>
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<ul>
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<nav class="cl-effect-5">
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<li><a  href="https://2017.igem.org/Team:GZHS-United"><span data-hover="HOME">HOME</span></a></li>
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<span data-hover="TEAM">TEAM</span>
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</a>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Team">Team</a>
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</li>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Attributions">Attributions</a>
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</li>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Collaborations">Collaborations</a>
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</li>
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</ul>
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<li class="dropdown">
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<a  class="dropdown-toggle" id="dropdownMenu2" data-toggle="dropdown" href="#">
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<span data-hover="PROJECT">PROJECT</span>
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Description">Description</a>
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</li>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Design">Design</a>
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</li>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Experiments">Experiments</a>
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</li>
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</ul>
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<li class="dropdown">
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</li>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Model">Modeling</a>
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</li>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Demonstrate">Demonstrate</a>
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</li>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="#">Improve</a>
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</li>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="#">Contribution</a>
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<span data-hover="PARTS">PARTS</span>
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</ul>
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<li class="dropdown">
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<a  class="dropdown-toggle" id="dropdownMenu5" data-toggle="dropdown" href="#">
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<span data-hover="NOTEBOOK">NOTEBOOK</span>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Gallery">Gallery</a>
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</li>
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Timeline">Timeline</a>
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</li>
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/InterLab">InterLab</a>
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Safety">Safety</a>
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</li>
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</ul>
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<a  class="dropdown-toggle" id="dropdownMenu7" data-toggle="dropdown" href="#">
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<span data-hover="HUMAN_PRACTICE" class="dropdown_title">HUMAN PRACTICE</span>
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</a>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/HP/Silver">Silver</a>
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</li>
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<li role="presentation">
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/HP/Gold_Integrated">Gold & Integrated</a>
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</li>
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Engagement">Public Engagement</a>
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<a role="menuitem" tabindex="-1" href="https://2017.igem.org/Team:GZHS-United/Entrepreneurship">Entrepreneurship</a>
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</li>
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</ul>
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</li>
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</nav>
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</ul>
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<!-- script for menu -->
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<!--script-nav-->
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<script>
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$("span.menu").click(function(){
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$(".top-menu ul").slideToggle("slow" , function(){
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});
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});
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</script>
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<!-- //script for menu -->
  
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<h1> Safety </h1>
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<!-- script-for sticky-nav -->
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<script>
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$(document).ready(function() {
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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</div>
  
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</div>
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</div>
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</div>
 
</div>
 
</div>
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<div class="content" id="satety_content">
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<div class="container">
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<div class="row">
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<h1>Satety</h1>
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<h2>Safety design in our project:</h2>
 +
<ol>
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<li>Products:  Cell free product , containing purified proteins , cat foods and agarose , therefore no risk of mutation, gene flow or other hazards associate with living cells.</li>
 +
<li>Bio-chasis:  E.coli, the most commonly used bio-chasis in genetic engineering,  pathogenic free.</li>
 +
<li>Proteins: Cry4Ba and Mtx1 which were synthesized in our experiment specificly target mosquito larvae , harming neither plant nor mammals. </li>
 +
<li>Resistance: Using a chemical as pesticide is risky, for mosquito can easily develop resistance to this pesticide. Biopesticide can impose a lower selective pressure in mosquito so mosquito is less likely to develop resistance.  By combining cry4Ba and Mtx1 together as bio-pesticide, we can reduce the risk of resistance considerably.</li>
 +
</ol>
 +
 +
<h2>General rules and safety measures</h2>
 +
<ol>
 +
<li>No food or drink in the lab.</li>
 +
<li>Always wearing lab coat, confining long hair and loose clothing. Closed shoes in the lab. </li>
 +
<li>Wear safety goggles and mask while handling with toxic materials and chemicals</li>
 +
<li>Safe disposal of contaminated wastes under local regulations</li>
 +
<li>Inactivate all living things before disposal</li>
 +
<li>Work under proper ventilation only</li>
 +
<li>All lab materials are kept and used only in the lab</li>
 +
<li>The feeding areas of larvae must be separated. There was no adult mosquito emerged, only larvae were involved in the experiment.</li>
 +
<li>he biological material we used in the experiment, including bacteria and larvae, must be microwaved so as to kill them before pouring them into the sewer.</li>
 +
<p>Policies and regulations</p>
  
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<h2>Material management:</h2>
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<div class="column full_size">
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<ol>
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<h5>Safe Project Design</h5>
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<li>The mosquito eggs we used in the experiment are the lab susceptible strain,which were collect from Foshan in the 1980s andhad been kept in the lab for over 30 years without  exposure to any insecticide. </li>
−
 
+
<li>We kept on monitoring the larvae during the experiment. If mosquito larvae survived to the pupate stage, , we will execute them so as to avoid releasing adult mosquito in our lab.</li>
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
<li>All the biological materials (including eggs, larvae and pupae) will be micorwaved for at least five minutes before being discarded.</li>
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+
</ol>
−
<ul>
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<h2>lab safety course</h2>
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<li>Choosing a non-pathogenic chassis</li>
+
<p>Members in our team have taken  lab safety courses conducted by Professor Honglei Jin, on safety rules , risks and waste dispersal procedures in the lab. The topics in our safety training include the follow sections:</p>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<h2>lab safety course</h2>
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<li>Including an "induced lethality" or "kill-switch" device</li>
+
<p>Members in our team have taken  lab safety courses conducted by Professor Honglei Jin, on safety rules , risks and waste dispersal procedures in the lab. The topics in our safety training include the follow sections:</p>
−
</ul>
+
<ol>
−
 
+
<li>Non-biological operations:
 +
<ol>
 +
<li>Handling toxic chemicals</li>
 +
<li>Emergency measures </li>
 +
<li>Instruments and facilities operation principles</li>
 +
<li>List of common toxic chemicals in lab</br>
 +
(Like gold view and acrylamide)</li>
 +
</ol>
 +
</li>
 +
<li>Biological operations:
 +
<ol>
 +
<li>Potential dangers of engineering bacterium</li>
 +
<li>Waste materials handling measures</li>
 +
<li>Emission rules</li>
 +
</ol>
 +
</li>
 +
</ol>
 +
</div>
 +
</div>
 
</div>
 
</div>
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</body>
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<div class="column half_size">
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<script>
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<h5>Safe Lab Work</h5>
+
$(document).ready(function(){
−
 
+
dropdownOpen();
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
function dropdownOpen() {
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var $dropdownLi = $('li.dropdown');
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</div>
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$dropdownLi.mouseover(function() {
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$(this).addClass('open');
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<div class="column half_size">
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}).mouseout(function() {
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<h5>Safe Shipment</h5>
+
$(this).removeClass('open');
−
 
+
});
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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}
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</div>
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});
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</script>
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</html>
 
</html>

Revision as of 14:59, 15 October 2017

satety

Satety

Safety design in our project:

  1. Products: Cell free product , containing purified proteins , cat foods and agarose , therefore no risk of mutation, gene flow or other hazards associate with living cells.
  2. Bio-chasis: E.coli, the most commonly used bio-chasis in genetic engineering, pathogenic free.
  3. Proteins: Cry4Ba and Mtx1 which were synthesized in our experiment specificly target mosquito larvae , harming neither plant nor mammals.
  4. Resistance: Using a chemical as pesticide is risky, for mosquito can easily develop resistance to this pesticide. Biopesticide can impose a lower selective pressure in mosquito so mosquito is less likely to develop resistance. By combining cry4Ba and Mtx1 together as bio-pesticide, we can reduce the risk of resistance considerably.

General rules and safety measures

  1. No food or drink in the lab.
  2. Always wearing lab coat, confining long hair and loose clothing. Closed shoes in the lab.
  3. Wear safety goggles and mask while handling with toxic materials and chemicals
  4. Safe disposal of contaminated wastes under local regulations
  5. Inactivate all living things before disposal
  6. Work under proper ventilation only
  7. All lab materials are kept and used only in the lab
  8. The feeding areas of larvae must be separated. There was no adult mosquito emerged, only larvae were involved in the experiment.
  9. he biological material we used in the experiment, including bacteria and larvae, must be microwaved so as to kill them before pouring them into the sewer.

    10. Policies and regulations

    Material management:

    1. The mosquito eggs we used in the experiment are the lab susceptible strain,which were collect from Foshan in the 1980s andhad been kept in the lab for over 30 years without exposure to any insecticide.
    2. We kept on monitoring the larvae during the experiment. If mosquito larvae survived to the pupate stage, , we will execute them so as to avoid releasing adult mosquito in our lab.
    3. All the biological materials (including eggs, larvae and pupae) will be micorwaved for at least five minutes before being discarded.

    lab safety course

    Members in our team have taken lab safety courses conducted by Professor Honglei Jin, on safety rules , risks and waste dispersal procedures in the lab. The topics in our safety training include the follow sections:

    lab safety course

    Members in our team have taken lab safety courses conducted by Professor Honglei Jin, on safety rules , risks and waste dispersal procedures in the lab. The topics in our safety training include the follow sections:

    1. Non-biological operations:
      1. Handling toxic chemicals
      2. Emergency measures
      3. Instruments and facilities operation principles
      4. List of common toxic chemicals in lab
        (Like gold view and acrylamide)
    2. Biological operations:
      1. Potential dangers of engineering bacterium
      2. Waste materials handling measures
      3. Emission rules