Difference between revisions of "Team:BIT-China/Notebook"
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| + | {{BIT-China}} | ||
| + | <html> | ||
| + | <style> | ||
| + | .title-h2 { | ||
| + | position: relative; | ||
| + | top: 86px; | ||
| + | width: 100%; | ||
| + | padding-top: 86px; | ||
| + | margin: auto; | ||
| + | text-align: center; | ||
| + | font-weight: bold; | ||
| + | letter-spacing: 3px; | ||
| + | text-decoration: none; !important | ||
| + | } | ||
| + | .my-pdf { | ||
| + | margin: auto; | ||
| + | padding-top: 100px; | ||
| + | min-width: 856px; | ||
| + | margin-bottom: 200px; | ||
| + | } | ||
| + | </style> | ||
| + | <body> | ||
| + | <div class="my-banner" id="top"> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/e/ec/T--BIT-China--2017laboratory.jpg" alt=""> | ||
| + | </div> | ||
| + | <section class="content_container" id="mytop"> | ||
| + | <h2 class="title-h2" id="mytop">NOTEBOOK</h2> | ||
| + | <h3 class="title-h3">Breakthrough</h3> | ||
| + | <h4 class="title-h4">2.14-4.14: Recruiting team & Brainstorm</h4> | ||
| + | <h4 class="title-h4">4.14-6.20: core part synthesis and heterologous expression</h4> | ||
| + | <p class="my-content-li2">4.14-5.2 : core part-T1R2/T1R3 synthesis completely through OE-PCR</p> | ||
| + | <p class="my-content-li2">4.30-6.20 : heterologous expression of T1R2/T1R3 and characteristic</p> | ||
| + | <h4 class="title-h4">4.30-5.15：modeling</h4> | ||
| + | <p class="my-content-li2">4.30-5.5：finish several sweeteners’ as well as T1R2/T1R3 PDB file construction. Different sweeteners and numbers are docking to the sweet receptor T1R2/T1R3.</p> | ||
| + | <p class="my-content-li2">5.5-5.15：MAPK pathway signal modeling</p> | ||
| + | <h4 class="title-h4">5.2-8.20: pathway optimization</h4> | ||
| + | <p class="my-content-li2">5.2-7.1 : single gene knock out</p> | ||
| + | <p class="my-content-li2">7.1-8.5 : knock out gene combination</p> | ||
| + | <p class="my-content-li2">8.5-8.20 : engineered strain characterization</p> | ||
| + | <h4 class="title-h4">8.21-10.20: new pathway verification</h4> | ||
| + | <p class="my-content-li2">8.21-9.13 : diction circuit establishment</p> | ||
| + | <p class="my-content-li2">9.13-9.26 : new pathway verification</p> | ||
| + | <p class="my-content-li2">9.27-10.10 : sweet meter verification</p> | ||
| + | <p class="my-content-li2">10.8-10.20 : parts standardization</p> | ||
| + | |||
| + | <div class="my-main-container"> | ||
| + | <object class="my-pdf center-block" data="https://static.igem.org/mediawiki/2017/7/7c/Bit-china-notebook.pdf" type="application/pdf" width="70%" height="900px" internalinstanceid="11" title=""> | ||
| + | <p>It appears you don't have a PDF plugin for this browser. | ||
| + | No biggie... you can <a href="https://static.igem.org/mediawiki/2017/7/7c/Bit-china-notebook.pdf">click here to | ||
| + | download the PDF file.</a></p> | ||
| + | </object> | ||
| + | </div> | ||
| + | <p class="my-content-p">For more details,please see our <a href="https://2017.igem.org/Team:BIT-China/Project/">project</a>.</p> | ||
| + | |||
| + | <div class="article-nav"> | ||
| + | <a href="https://2017.igem.org/Team:BIT-China/Material" class="article-nav-left"> | ||
| + | <img class="article-caption" src="https://static.igem.org/mediawiki/2017/a/a4/T--BIT-China--2017previous.png" alt=""> | ||
| + | </a> | ||
| + | <a href="https://2017.igem.org/Team:BIT-China/Protocol" class="article-nav-right"> | ||
| + | <img class="article-caption" src="https://static.igem.org/mediawiki/2017/6/6f/T--BIT-China--2017next.png" alt=""> | ||
| + | </a> | ||
| + | </div> | ||
| + | </section> | ||
| + | |||
| + | |||
| + | <a class="my-backTop" href="#mytop"> | ||
| + | <span>TOP</span> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/f/fd/Bit-china-2017backTop.png" alt=""> | ||
| + | </a> | ||
| + | </body> | ||
| + | </html> | ||
| + | {{BIT-China/footer}} | ||
Revision as of 11:11, 24 October 2017
NOTEBOOK
Breakthrough
2.14-4.14: Recruiting team & Brainstorm
4.14-6.20: core part synthesis and heterologous expression
4.14-5.2 : core part-T1R2/T1R3 synthesis completely through OE-PCR
4.30-6.20 : heterologous expression of T1R2/T1R3 and characteristic
4.30-5.15：modeling
4.30-5.5：finish several sweeteners’ as well as T1R2/T1R3 PDB file construction. Different sweeteners and numbers are docking to the sweet receptor T1R2/T1R3.
5.5-5.15：MAPK pathway signal modeling
5.2-8.20: pathway optimization
5.2-7.1 : single gene knock out
7.1-8.5 : knock out gene combination
8.5-8.20 : engineered strain characterization
8.21-10.20: new pathway verification
8.21-9.13 : diction circuit establishment
9.13-9.26 : new pathway verification
9.27-10.10 : sweet meter verification
10.8-10.20 : parts standardization
For more details,please see our project.
