Difference between revisions of "Team:Lanzhou/Collaborations"

Line 39: Line 39:
 
<img src="https://static.igem.org/mediawiki/2017/thumb/1/1a/Lanzhou_wiki_Lab_neu_1.png/800px-Lanzhou_wiki_Lab_neu_1.png" alt="">
 
<img src="https://static.igem.org/mediawiki/2017/thumb/1/1a/Lanzhou_wiki_Lab_neu_1.png/800px-Lanzhou_wiki_Lab_neu_1.png" alt="">
 
<figcaption>Figure 1. standardized Rho-OR1A1.</figcaption>
 
<figcaption>Figure 1. standardized Rho-OR1A1.</figcaption>
</figure>
 
<figure>
 
<img src="https://static.igem.org/mediawiki/2017/thumb/5/57/Lanzhou_wiki_Lab_neu_2.png/800px-Lanzhou_wiki_Lab_neu_2.png" alt="">
 
<figcaption>Figure 2. PstI, EcoRI double enzyme digestion.</figcaption>
 
 
</figure>
 
</figure>
 
<p class="mdc-typography--body2">
 
<p class="mdc-typography--body2">
Line 51: Line 47:
 
So NEU-China students helped us construct a hpRNA producing vector ecrL, which speeded up our vectors construction progress.  
 
So NEU-China students helped us construct a hpRNA producing vector ecrL, which speeded up our vectors construction progress.  
 
</p>
 
</p>
 +
<figure>
 +
<img src="https://static.igem.org/mediawiki/2017/thumb/5/57/Lanzhou_wiki_Lab_neu_2.png/800px-Lanzhou_wiki_Lab_neu_2.png" alt="">
 +
<figcaption>Figure 2. PstI, EcoRI double enzyme digestion.</figcaption>
 +
</figure>
 
<p class="mdc-typography--body2">
 
<p class="mdc-typography--body2">
 
<b>Really appreciate their support.</b>
 
<b>Really appreciate their support.</b>

Revision as of 05:48, 29 October 2017

Lanzhou

Lanzhou2017

NEU-China Visit their wiki

The cooperation with Northeastern University has begun since the summer vocation. We helped them complete a receptor and Working as a very kind and helpful team, they asked helping us in verifying one fuctional protein expression.

We helped NEU-China construct an olfactory receptor Rho-OR1A1. The 64th base pair from the initiation codon of OR1A1 contains an illegal enzyme site (EcoRI), which didn't meet up RFC10 standard. So we designed the primer with synonymous codon and successfully mutated the GAA to GAG.

Figure 1. standardized Rho-OR1A1.

In our project, we need various functional vectors for dsRNA production,but the gene sequence complementarity will cause secondary structure, which is impossible to synthsize gene for one time. The task of constructing vectors is heavy.

So NEU-China students helped us construct a hpRNA producing vector ecrL, which speeded up our vectors construction progress.

Figure 2. PstI, EcoRI double enzyme digestion.

Really appreciate their support.

BIT-China Visit their wiki

After we meet up through vedio conference with BIT-China, We exchanged ideas and confirmed our collaboration relationship.

In the light of helping each other, we assisted them to build a new type of promoter.

At the same time, they helped us verify one functional protein expression.

Figure 1. Mutant gene Pfus.

BIT-China project requires mRFP to characterize the size of the sweetness. Different sweeteners have different gradients of sweetness, they need to get mutations of the inducible promoter Pfus and change its strength.

So we helped them build a mutant gene of Pfus to make their project more responsive.

Figure 2. SDS-PAGE of pectinase.

In our project, pectinase and celluse play an important role in downstream circuits and we want to combine functional dsRNA with these two proteins and let them work together, which will make great sense to our subsequent appllication design.

They successfully verified the expression of pectinase. With their help, our project progress has been promoted.




NEU-China

BIT-China