Difference between revisions of "Team:BIT-China/Notebook"
| Line 27: | Line 27: | ||
<h2 class="title-h2" id="mytop">NOTEBOOK</h2> | <h2 class="title-h2" id="mytop">NOTEBOOK</h2> | ||
<h3 class="title-h3">Breakthrough</h3> | <h3 class="title-h3">Breakthrough</h3> | ||
| − | <h4 class="title-h4">2.14-4.14: | + | <h4 class="title-h4">2.14-4.14:team recruitment & brainstorm</h4> |
<h4 class="title-h4">4.14-6.20: core part synthesis and heterologous expression</h4> | <h4 class="title-h4">4.14-6.20: core part synthesis and heterologous expression</h4> | ||
| − | <p class="my-content-li2">4.14-5.2 : | + | <p class="my-content-li2">4.14-5.2: core part-T1R2/T1R3 synthesis completely through OE-PCR.</p> |
| − | <p class="my-content-li2">4.30-6.20 : heterologous expression of T1R2/T1R3. <a href="https://2017.igem.org/Team:BIT-China/Project/Sense">Click here to see more details.</a></p> | + | <p class="my-content-li2">4.30-6.20: heterologous expression of T1R2/T1R3. <a href="https://2017.igem.org/Team:BIT-China/Project/Sense">Click here to see more details.</a></p> |
<h4 class="title-h4">4.30-5.15：modeling</h4> | <h4 class="title-h4">4.30-5.15：modeling</h4> | ||
| − | <p class="my-content-li2">4.30-5. | + | <p class="my-content-li2">4.30-5.5: finished several sweeteners'docking as well as T1R2/T1R3 PDB file construction. Different sweeteners docked to the sweet taste receptor T1R2/T1R3.<a href="https://2017.igem.org/Team:BIT-China/Model/Docking">Click here to see more details.</a></p> |
| − | <p class="my-content-li2">5.5-5. | + | <p class="my-content-li2">5.5-5.15: MAPK pathway signal modeling. <a href="https://2017.igem.org/Team:BIT-China/Model/GPCRpathway">Click here to see more details.</a></p> |
<h4 class="title-h4">5.2-8.20: pathway optimization</h4> | <h4 class="title-h4">5.2-8.20: pathway optimization</h4> | ||
| − | <p class="my-content-li2">5.2-7.1 : single gene knockout</p> | + | <p class="my-content-li2">5.2-7.1: single gene knockout</p> |
| − | <p class="my-content-li2">7.1-8.5 :any two of the three genes knockout and three genes knockout<a href="https://2017.igem.org/Team:BIT-China/Project/Transduction">Click here to see more details.</a></p> | + | <p class="my-content-li2">7.1-8.5: any two of the three genes knockout and three genes knockout<a href="https://2017.igem.org/Team:BIT-China/Project/Transduction">Click here to see more details.</a></p> |
| − | <p class="my-content-li2">8.5-8.20 : characterization of engineered strains </p> | + | <p class="my-content-li2">8.5-8.20: characterization of engineered strains </p> |
<h4 class="title-h4">8.21-10.20:Verification of the new pathway </h4> | <h4 class="title-h4">8.21-10.20:Verification of the new pathway </h4> | ||
| − | <p class="my-content-li2">8.21-9.13 : establishment of the detection circuit .<a href="https://2017.igem.org/Team:BIT-China/Project/Detection">Click here to see more details.</a></p> | + | <p class="my-content-li2">8.21-9.13: establishment of the detection circuit .<a href="https://2017.igem.org/Team:BIT-China/Project/Detection">Click here to see more details.</a></p> |
| − | <p class="my-content-li2">9.13-9.26 : verification of the new pathway </p> | + | <p class="my-content-li2">9.13-9.26: verification of the new pathway </p> |
| − | <p class="my-content-li2">9.27-10.10 : sweetness meter verification</p> | + | <p class="my-content-li2">9.27-10.10: sweetness meter verification</p> |
| − | <p class="my-content-li2">10.8-10.20 : parts standardization</p> | + | <p class="my-content-li2">10.8-10.20: parts standardization</p> |
Revision as of 11:21, 30 October 2017
NOTEBOOK
Breakthrough
2.14-4.14:team recruitment & brainstorm
4.14-6.20: core part synthesis and heterologous expression
4.14-5.2: core part-T1R2/T1R3 synthesis completely through OE-PCR.
4.30-6.20: heterologous expression of T1R2/T1R3. Click here to see more details.
4.30-5.15：modeling
4.30-5.5: finished several sweeteners'docking as well as T1R2/T1R3 PDB file construction. Different sweeteners docked to the sweet taste receptor T1R2/T1R3.Click here to see more details.
5.5-5.15: MAPK pathway signal modeling. Click here to see more details.
5.2-8.20: pathway optimization
5.2-7.1: single gene knockout
7.1-8.5: any two of the three genes knockout and three genes knockoutClick here to see more details.
8.5-8.20: characterization of engineered strains
8.21-10.20:Verification of the new pathway
8.21-9.13: establishment of the detection circuit .Click here to see more details.
9.13-9.26: verification of the new pathway
9.27-10.10: sweetness meter verification
10.8-10.20: parts standardization
For more details,please see our project.
