The number of biotechnological applications has skyrocketed and have evolved to be one of the most ubiquitous aspects in our daily life in the past years. From uses in food industry to dietary supplements and science, white biotechnology is everywhere.

That’s why we try to recreate a widespread natural phenomenon called metabolic channelling synthetically to increase the yield biochemical reactions. Metabolic channelling occurs when the enzymes responsible for a specific reaction are in close proximity to each other, and thereby decrease the diffusion time of the substrates (see animation below).

*Animation zur Verdeutlichung von metabolic channelling*

In our first method, we utilize the DNA-binding function of deadCas9 (dCas9) to bind two enzymes of the Indoleacetic acid (IAA) pathway, IAA tryptophan monooxygenase (IAAM) and indoleacetamide hydrolase (IaaH) on a DNA-scaffold which we introduce into E.coli. By binding the enzymes on DNA, we decrease the distance the substrates have to travel in the cytoplasm and hope to achieve a higher output of Indoleacetic acid.