Difference between revisions of "Team:Potsdam/Contribution"

(1. Fassung mit K2483000)
Line 14: Line 14:
 
             <div id="content">
 
             <div id="content">
 
<div align="justify">
 
<div align="justify">
−
Parts to be contributed: 005/006/007
+
 
−
<br> Maybe: 002
+
<b>BBa_K2483000 - IAA producing enzymes fused to RNA-binding proteins under control of Pveg2</b>
−
<br>
+
<br><br>
−
<br>
+
The idea of the contribution requirement of iGEM is to expand the uses and information available of different parts in the registry.
−
Participate in the Interlab Measurement Study (to be documented on your InterLab page) and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range. Teams who are working on improving the characterization of an existing part should document their experimental design here, along with an explanation for why they chose that part to improve. Data can also be shown here, but it MUST also be documented on the part's Main Page in the Registry.
+
−
<br>
+
−
<br>
+
−
<b>IAA producing enzymes fused to RNA-binding proteins under control of Pveg2</b>
+
 
<br><br>
 
<br><br>
−
This part is an upgrade to the part BBa_K515100 of iGEM Imperial College 2012 producing Indoleacetic acid with the enzymes IaaM (Indoleacetic Acid tryptophan monooxygenase) and IaaH (Indoleacetamide Hydrolase). Here we just fused the IAA enzymes to the RNA-binding proteins (RBPs) MS2 and PP7, respectively.
+
This part is an upgrade to the part BBa_K515100 of iGEM Imperial College 2012 producing Indoleacetic acid with the enzymes IaaM (Indoleacetic Acid tryptophan monooxygenase) and IaaH (Indoleacetamide Hydrolase). Here we fused the IAA enzymes to the RNA-binding proteins (RBPs) MS2 and PP7, respectively. We focused on giving this part a new function by being able to bind specific RNA domains.
 
<br><br>
 
<br><br>
  
−
We used this part to produce Indoleacetic Acid (IAA) in E.coli and increase the efficiency of the reaction by putting them in close proximity to each other (metabolic channelling). This was done with a dCas9 (BBa_K2483002) scaffold where the RNA-binding proteins bind to aptamer sequences added to the 3'-end of a sgRNA (BBa_K2483003) and the sgRNA binds to target cassettes where several of the sgRNA recognition sequences are located behind each other (BBa_K2483005 and BBa_K2483006).
+
We used this part to produce Indoleacetic Acid (IAA) in <i>E.coli</i> and increase the efficiency of the reaction by putting them in close proximity to each other (metabolic channelling). This was done with a dCas9 (BBa_K2483002) scaffold where the RNA-binding proteins bind to aptamer sequences added to the 3'-end of a sgRNA and the sgRNA binds to target cassettes where several of the sgRNA recognition sequences are consecutively arranged (BBa_K2483005 and BBa_K2483006). For the complete part with sgRNA, dCas9 and the IAA-enzymes, see BBa_K2483004.
 
</div>
 
</div>
 
</div>
 
</div>

Revision as of 15:12, 1 November 2017

No Sidebar - Escape Velocity by HTML5 UP

Contribution-Bronze
BBa_K2483000 - IAA producing enzymes fused to RNA-binding proteins under control of Pveg2

The idea of the contribution requirement of iGEM is to expand the uses and information available of different parts in the registry.

This part is an upgrade to the part BBa_K515100 of iGEM Imperial College 2012 producing Indoleacetic acid with the enzymes IaaM (Indoleacetic Acid tryptophan monooxygenase) and IaaH (Indoleacetamide Hydrolase). Here we fused the IAA enzymes to the RNA-binding proteins (RBPs) MS2 and PP7, respectively. We focused on giving this part a new function by being able to bind specific RNA domains.

We used this part to produce Indoleacetic Acid (IAA) in E.coli and increase the efficiency of the reaction by putting them in close proximity to each other (metabolic channelling). This was done with a dCas9 (BBa_K2483002) scaffold where the RNA-binding proteins bind to aptamer sequences added to the 3'-end of a sgRNA and the sgRNA binds to target cassettes where several of the sgRNA recognition sequences are consecutively arranged (BBa_K2483005 and BBa_K2483006). For the complete part with sgRNA, dCas9 and the IAA-enzymes, see BBa_K2483004.