Difference between revisions of "Team:Pasteur Paris/Protocol"

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   position: fixed;
 
   position: fixed;
 
   left: 40px;
 
   left: 40px;
   top: 35%;
+
   top: 28%;
 
}
 
}
  
 
.outer6 {
 
.outer6 {
 
   position: fixed;
 
   position: fixed;
   left: 40px;
+
   left: 33px;
   top: 44%;
+
   top: 42.5%;
 +
  text-align: center;
 
}
 
}
  
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   position: fixed;
 
   position: fixed;
 
   left: 40px;
 
   left: 40px;
   top: 53.7%;
+
   top: 50.7%;
  text-align: center;
+
 
}
 
}
  
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     position:relative;
 
     position:relative;
 
     top:5px;
 
     top:5px;
     left:33%;
+
     left:25%;
 
     display:none;
 
     display:none;
 
     height:3px;
 
     height:3px;
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     <div class="inner"; onmouseover="apparitionbaton()"; onmouseout="deletebaton()">
 
     <div class="inner"; onmouseover="apparitionbaton()"; onmouseout="deletebaton()">
 
       <div class="list rotate">  
 
       <div class="list rotate">  
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Team">Our Team</a>
+
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Team">Our Team </a>
 
         <div id="batonbleu">
 
         <div id="batonbleu">
 
         </div>
 
         </div>
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     <div class="inner"; onmouseover="apparitionbaton3()"; onmouseout="deletebaton3()">
 
     <div class="inner"; onmouseover="apparitionbaton3()"; onmouseout="deletebaton3()">
 
       <div class="list rotate">  
 
       <div class="list rotate">  
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Project">Overview</a>
+
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Entrepreneurship">Entrepreneurship</a>
 
         <div id="batonbleu3">
 
         <div id="batonbleu3">
 +
        </div>
 +
      </div>
 +
  </div>
 +
  </div>
 +
    <div class="outer3a">
 +
    <div class="inner"; onmouseover="apparitionbaton3a()"; onmouseout="deletebaton3a()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/Engagement">Engagement</a>
 +
        <div id="batonbleu3a">
 
         </div>
 
         </div>
 
       </div>
 
       </div>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
</div>
+
   <div class="outer3b">
 
+
     <div class="inner"; onmouseover="apparitionbaton3b()"; onmouseout="deletebaton3b()">
<div id="Design" style="display:none">
+
   <div class="outer4">
+
     <div class="inner"; onmouseover="apparitionbaton4()"; onmouseout="deletebaton4()">
+
 
       <div class="list rotate">  
 
       <div class="list rotate">  
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Design">Science</a>
+
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Model">Model</a>
         <div id="batonbleu4">
+
         <div id="batonbleu3b">
 
         </div>
 
         </div>
 
       </div>
 
       </div>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
 +
</div>
 +
 +
<div id="Design" style="display:none">
 
   <div class="outer5">
 
   <div class="outer5">
 
     <div class="inner"; onmouseover="apparitionbaton5()"; onmouseout="deletebaton5()">
 
     <div class="inner"; onmouseover="apparitionbaton5()"; onmouseout="deletebaton5()">
 
       <div class="list rotate">  
 
       <div class="list rotate">  
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Design">Product</a>
+
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Design">Design Methodology</a>
 
         <div id="batonbleu5">
 
         <div id="batonbleu5">
 
         </div>
 
         </div>
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     <div class="inner"; onmouseover="apparitionbaton6()"; onmouseout="deletebaton6()">
 
     <div class="inner"; onmouseover="apparitionbaton6()"; onmouseout="deletebaton6()">
 
       <div class="list rotate">  
 
       <div class="list rotate">  
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Design">Scenario</a>
+
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Applied_Design">Applied<br>Design</a>
 
         <div id="batonbleu6">
 
         <div id="batonbleu6">
 
         </div>
 
         </div>
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     <div class="inner"; onmouseover="apparitionbaton7()"; onmouseout="deletebaton7()">
 
     <div class="inner"; onmouseover="apparitionbaton7()"; onmouseout="deletebaton7()">
 
       <div class="list rotate">  
 
       <div class="list rotate">  
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Design">Methodology</a>
+
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Science">Science</a>
 
         <div id="batonbleu7">
 
         <div id="batonbleu7">
 
         </div>
 
         </div>
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     <div class="inner"; onmouseover="apparitionbaton8b()"; onmouseout="deletebaton8b()">
 
     <div class="inner"; onmouseover="apparitionbaton8b()"; onmouseout="deletebaton8b()">
 
       <div class="list rotate">  
 
       <div class="list rotate">  
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Results">Results</a>
+
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Demonstrate">Demonstrate</a>
 
         <div id="batonbleu8b">
 
         <div id="batonbleu8b">
 
         </div>
 
         </div>
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     <div class="inner"; onmouseover="apparitionbaton9()"; onmouseout="deletebaton9()">
 
     <div class="inner"; onmouseover="apparitionbaton9()"; onmouseout="deletebaton9()">
 
       <div class="list rotate">  
 
       <div class="list rotate">  
         <a href="https://2017.igem.org/Team:Pasteur_Paris/InterLab">Interlab</a>
+
         <a href="https://2017.igem.org/Team:Pasteur_Paris/Interlab">Interlab</a>
 
         <div id="batonbleu9">
 
         <div id="batonbleu9">
 
         </div>
 
         </div>
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         <a href="https://2017.igem.org/Team:Pasteur_Paris/HP/Collaborations">Collaborations</a>
 
         <a href="https://2017.igem.org/Team:Pasteur_Paris/HP/Collaborations">Collaborations</a>
 
         <div id="batonbleu13">
 
         <div id="batonbleu13">
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
  <div class="outer13b">
 +
    <div class="inner"; onmouseover="apparitionbaton13b()"; onmouseout="deletebaton13b()">
 +
      <div class="list rotate">
 +
        <a href="https://2017.igem.org/Team:Pasteur_Paris/HP/Silver">Human Practices</a>
 +
        <div id="batonbleu13b">
 
         </div>
 
         </div>
 
       </div>
 
       </div>

Revision as of 03:15, 2 November 2017

Protocols

Agarose Gel Preparation

Bacteria Transformation

Bacteria Transformation (Interlab)

Dephosphorylation of DNA

DNA Digestion

DNA Electrophoresis and Gel Extraction

Ligation of Plasmid with DNA Insert

Liquid Culture and Induction of Protein Expression

Liquid Culture for a Miniprep on Transformed Bacteria

Measurement of DNA Concentration

Miniprep

Small Scale Bacteria Lysis and SDS PAGE Gel Migration

During our lab work, we used the following protocols. You can download them all.