Team:Pasteur Paris/Protocol


Agarose Gel Preparation

Bacteria Transformation

Bacteria Transformation (Interlab)

Dephosphorylation of DNA

DNA Digestion

DNA Electrophoresis and Gel Extraction

Ligation of Plasmid with DNA Insert

Liquid Culture and Induction of Protein Expression

Liquid Culture for a Miniprep on Transformed Bacteria

Measurement of DNA Concentration


Small Scale Bacteria Lysis and SDS PAGE Gel Migration

During our lab work, we used the following protocols. You can download them all.