| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K2377000 | DNA | Intron loop with Aphids specificity | Yuan Wang | 194 |
| BBa_K2377001 | DNA | Intron loop with Arabidopsis specificity | Yuan Wang | 106 |
| BBa_K2377002 | DNA | Celluse coding sequence | Yuan Wang | 1500 |
| BBa_K2377003 | Coding | Pectinase coding sequence | Yuan Wang | 1047 |
| BBa_K2377010 | Coding | EcrL1 , the sense fragment of Ecr gene | He Jindian | 523 |
| BBa_K2377011 | DNA | EcrL2 , the anti-sense fragment of Ecr gene | He Jindian | 523 |
| BBa_K2377012 | Coding | Trxz sense fragment | He Jindian | 552 |
| BBa_K2377013 | DNA | Trxz anti-sense fragment | He Jindian | 552 |
| BBa_K2377014 | Regulatory | zarp, zinc two-component relulatory system | He Jindian | 201 |
| BBa_K2377015 | DNA | loop120 | Yuan Wang | 121 |
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K2377004 | DNA | Thioredoxin Z with two reverse T7 promoters and double terminators to generate dsRNA | Ren Yi | 904 |
| BBa_K2377005 | DNA | TrxzL, A developed element of generate dsRNA | He Jindian | 1401 |
| BBa_K2377006 | DNA | Replace intron loop with loop120( selected form A-1 chloramphenicol O-acetyltransferase) in BBa_K2377005 | He Jindian | 1386 |
| BBa_K2377007 | DNA | EcrL, one element to genarete Ecr hpRNA | He Jindian | 1358 |
| BBa_K2377008 | DNA | ecrN | He Jindian | 1431 |
| BBa_K2377009 | DNA | zrap ribB | He Jindian | 1029 |
Main part
BBa_K2377004
This one is initial part in our project. It is made up of Thioredoxin z (TRXz) gene fragment with two reverse T7 promoters and double terminators. And some research shows the deletion of TRX z expression generates a PEP-deficient phenotype and renders the plants incapable to grow autotrophically and their leaves will turn yellow.
BBa_K2377005
As the intron sequence will be sliced during the pre-mRNA processing and the stability is much higher than dsRNA and hpRNA with a normal loop according to a relevant paper, it will be a better option for us to silence the target sequene.
BBa_K2377007
Acyrthosiphon pisum ecdysone receptor (Ecr) is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003383721.1). ECR silencing can effectively reduce the expression of vital genes for Aphids development, thereby reducing the survival rate of insects and reproduction rate. This part is the sense arm of EcrL with loop120.
BBa_K2377002/BBa_K2377003
We design this two part for helping our dsRNA invade plant.
