Team:Lanzhou/Description
Lanzhou
Description
Although the war between desertification and human beings has never been halted, the existing methods are highly time-consuming and funds-costing such as physical approaches with straw barriers and chemical ones with surface immobilization. To change the situation, we are to develop a novel biological way with the technology and theory based on Synthetic Biology: Produce a engineered strain immobilizing sands and preventing evaporation of water to delay the process of desertification.
Background
Although the war between desertification and human beings has never been halted, the existing methods are highly time-consuming and funds-costing such as physical approaches with straw barriers and chemical ones with surface immobilization. To change the situation, we are to develop a novel biological way with the technology and theory based on Synthetic Biology: Produce a engineered strain immobilizing sands and preventing evaporation of water to delay the process of desertification.
Abstract
Weeds and insects are most important damages to the crop yield in the world. Due to the resistance and pollution problems, the increasing numbers of herbicides and insecticides have been losing functions during these years. RNA interference (RNAi), the novel molecular technology that used for gene knockdown has been showing the big potential in the agricultural field especially for insect control. However, the new RNAi-mediated herbicide is still demanded. Here, we report a new strategy to control weeds and insects at the same time by RNAi technology. To avoid potential off-target hazards and increase control effiency, we utilized BLAST and tandem RNAi technology to silence specific sequence in multiple targets. Meanwhile, we utilize E.coli to produce enough dsRNA in fermentation cylinder based on quorum sensing system
Project
- Develop an engineered strain which can secrete colonic acid(one kind of exopolysaccharides preventing water vaporing and forming soil aggregation) using E.coli as the microbe chassis
- Release different amount of colonic acid according to temperature changes
- Produce a novel biological bacterial fertilizer with this engineered strain
- Develop a general secreting system which can be used under different microbe chassis.
References
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