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After this, in order to get the relationship between the RFP intensity and the concentration of arabinose more intuitive, we also got this curve about the relationship between RFP intensity and arabinose when the RFP intensity was stable.

From this picture, we can know the "in-promoter" activity have a relationship with the different concentrations of the arabinose. That means when we add different concentrations of arabinose, the concentration of inhibitor will also be different. We regarded the concentration of arabinose 0.003% as a threshold, the corresponding expression of RFP (stands for killer) is about 12.05 units. At this point the expression of killer is regarded as just be repressed. Once the concentration of inhibitor decreases further, the killer gene can start to work. Conversely, more inhibitor remains, the killer will be repressed harder. Thus we can say the "in-promoter" can have different responses to the inhibitor.