Team:UNOTT/Model

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EMTPY

Modelling

When developing Key.Coli, we found it was important to mathematically model possible situations so we could investigate the effects of different situations we might encounter as well as representing every single chemical reaction which could expect.
This information was used by the wet lab to assist them by informing them in what to expect. This was done through the use of programming to create visual graphs.
These models can be accessed from our GitHub page
The models weren't perfect at first: refinement from lab results helped to optimize and correct the models

Our aims when creating the models were the following:

  1. To assist the processes within the Wet Lab by informing them and allowing for simulations. This would be especially useful when predicting the required fluorescence
  2. Test our biological systems with conditions that might not be possible to replicate in a lab environment. This allows us to future proof our methods as well as identify any vulnerabilities
    3. In order to achieve these aims, it was decided to create a simulation for measuring fluorescence intensity when given parameters such as protein concentrations and wavelengths of lasers added.