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...>Testing the performance of phiC31 recombinase using the register assembly construct with the attachment sites PxB.</p>
...tutive expression of phiC31 in the plant. c) Graphic representation of the construct comprised by a promoter and a terminator in opposite directions flanked by
50 KB (6,682 words) - 03:33, 2 November 2017
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<br> (2) Endolysin construct finish
<br> (5) Backbone construct-3NSII, 5NSII,AMP, B0015, and ORI (PCR: dream taq)<br>
26 KB (3,130 words) - 16:14, 28 November 2017
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...rror, so instead we performed 2-step NEB Phusion HF PCR of pSB1C3 with RFP construct</p>
<li>Performed Phusion PCR of pSB1C3 with RFP construct using psB1C3 #2 and the pKDL071 FWD/RVS primers
76 KB (12,349 words) - 20:03, 14 December 2017
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...ycin. The enzyme of this step is BsmBI again. This level is useful, if the construct you are designing requires multiple genes to be transformed into one yeast
...Cross <i>et al.</i>, published in 2017, in which they present a synthetic construct of the Pex5 protein, partly <i>Arabidopsis thaliana</i> and partly <i>Physc
199 KB (29,417 words) - 03:20, 2 November 2017
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...ldenBraid multipartite assembly reaction was performed to create a genetic construct composed by the transcriptional factor, PIF6, fused to a DNA-binding domain
...ression along time for different treatments, including the light-inducible construct (Etr8) under white-light conditions, and a weak constitutive promoter and a
51 KB (6,067 words) - 19:12, 14 December 2017
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.... The construct used for cutting <i>STE2</i> is shown in Figure 2, and the construct used for cutting <i>STE3</i> in Figure 3.
<figcaption><b>Figure 2.</b> gRNA construct for deletion of <i>STE2</i>.
253 KB (35,104 words) - 15:59, 30 October 2017
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<li style="font-family: Rubik">Designed, and began to construct, a variety of framework compatible systems, including a synthetic promoter
...moter was added to enable expression to be under the control of IPTG. This construct was then submitted to IDT for synthesis as a gBlock.
184 KB (26,744 words) - 19:53, 1 November 2017
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...moter was added to enable expression to be under the control of IPTG. This
construct was then submitted to IDT for synthesis as a gBlock.
...replacing the IPTG sensing system in the original detector module with the
construct detailed above, creating part <a href="http://parts.igem.org/Part:BBa_K2205
185 KB (26,866 words) - 22:39, 1 November 2017
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...subject, and also they get information about yeast extraction and protein construct.",,,,Consulted experts to gain more information on cyanobacteria and mathem
...ext two exchanges mainly focused on cooperation</li><li> Help BNU-China to construct a part of the gene circuit, and meanwhile, BNU-China helps with the charact
1.09 MB (163,642 words) - 17:36, 30 October 2017
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<p>Assess robustness of a GFP reporter construct in a range of environments.</p>
...ly. The Golden Gate assembly method successfully produced the GFP reporter construct, however it involved more steps and was more time consuming than Gibson ass
20 KB (2,994 words) - 21:41, 1 November 2017
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...ed split-luciferase system characterized by the 2015 Peking iGEM team, our construct consists of a promoter reporter system that looks at two downstream product
...ia in their intestines are influencing on protein nutrition. We thought to construct a pathway to synthesize amino acids from nitrogen in E.coli, introduce it i
400 KB (63,299 words) - 15:37, 15 August 2018
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...(Kittleson, 2012), which was essential both to obtain P1 particles and to construct each complete CRISPR system.</p>
engineered phagemid would need to become a P1 particle and construct whichever CRISPR system we have inserted into it within an <em>E. coli</em>
25 KB (3,973 words) - 20:43, 25 November 2017
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...lls (control) (15min) or not mixed in the presence or absence of the EL222 construct. Cell mixtures would have then be passed through a particle sizer with a ra
...fluorescence microscopy. We also included a His-Tag within all SpyCatcher construct variants to enable in-vitro analysis of complexes formed with SpyTag varian
122 KB (15,402 words) - 02:31, 2 November 2017
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<td class="col0"><strong>Construct</strong></td><td class="col1">6.43 µg</td>
<td class="col0"><strong>Construct</strong></td><td class="col1">6.43 µg</td>
167 KB (25,036 words) - 21:56, 31 October 2017
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Construct standard structure of integrase int5 and int8.<br>
Construct the expression system of integrase TP901, int2, int3, int4, int5, int7, int
16 KB (1,672 words) - 13:45, 1 November 2017
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...ycin. The enzyme of this step is BsmBI again. This level is useful, if the construct you are designing requires multiple genes to be transformed into one yeast
...Baker <i>et al.</i>, published in 2017, in which they present a synthetic construct of the Pex5 protein, partly <i>Arabidopsis thaliana</i> and partly <i>Physc
105 KB (15,409 words) - 02:56, 2 November 2017
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...o take our chances by building a D.I.V.E.R.T. cassette as well as a helper construct (carrying the RT, FLP and the primer cassettes) according to the best of ou
...synthetic regulatory elements<sup>1–3</sup> to keep the synthesized construct short. The synthetic galactose-inducible minimal promoter we used (sequence
68 KB (10,146 words) - 17:37, 15 December 2017
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...ctroporated DNA (BRED) (Figure 1). In this technique a double stranded DNA construct containing the
...to be replaced. The host cell for recombineering of the phage and the DNA construct contains a plasmid conferring genes promoting recombination. The double-str
10 KB (1,666 words) - 23:47, 1 November 2017
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...ycin. The enzyme of this step is BsmBI again. This level is useful, if the construct you are designing requires multiple genes to be transformed into one yeast
...Baker <i>et al.</i>, published in 2017, in which they present a synthetic construct of the Pex5 protein, partly <i>Arabidopsis thaliana</i> and partly <i>Physc
98 KB (14,470 words) - 00:02, 2 November 2017
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<a href="#ConDPR" class="pageNavSm">
Construct Design</a>
<a href="#ConDBio" class="pageNavSm">
Construct Design</a>
55 KB (6,458 words) - 14:08, 1 November 2017
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<td>Simple, easiest to construct</td>
...espective promoter (PphlF), and a GFP reporter (BBa_K2477008). We grew our construct in different molarities of fusaric acid (0µM, 100µM, and 500 
232 KB (33,473 words) - 02:45, 2 November 2017
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<div class="hull-content">Our dCas13a - GFP construct was synthesized by Integrated DNA Technologies. The dCas13a gene sequence w
To improve imaging, the dCas13a - GFP construct was sequesteredTo reduce background noise, unbound dCas13a - GFP should be
24 KB (3,114 words) - 00:16, 2 November 2017
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construct.<br><br>
<li>Construct half of the flip-flop structure and express it in <i>E. coli.</i></li>
170 KB (16,709 words) - 06:32, 15 December 2017
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<div class="hull-content">Our dCas13a - GFP construct was synthesized by Integrated DNA Technologies. The dCas13a gene sequence w
To improve imaging, the dCas13a - GFP construct was sequesteredTo reduce background noise, unbound dCas13a - GFP should be
24 KB (3,037 words) - 23:41, 15 December 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
159 KB (17,948 words) - 20:12, 1 November 2017
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<h2 class="textheading1" style="float:center"><u> Full Construct Testing</u> </h2>
...of GFP, the data seemed to be a bit problematic. We took a few of our full construct colonies as well as a few wild type Kanamycin resistant colonies and we ino
12 KB (1,480 words) - 02:11, 2 November 2017
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...This figure shows restriction enzyme check electrophoresis result of NrtA construct.
<h4>Endolysin Construct</h4>
29 KB (4,011 words) - 19:07, 1 November 2017
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...licated many times and matured, this allows us to have more cells with the
construct that can produce more pigment faster.
...wasted on transcribing an overly-long mRNA transcript. The melA expression
construct is the improved part that we were able to characterize the most (see <a hre
12 KB (1,924 words) - 17:29, 1 November 2017
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...luorescence was measured 20 minutes after this pH shift. The asr-mCardinal construct was inducible by change from pH 7.0 to pH 5.0 and lower but no fluorescence
...s in LPM with pH 7 overnight. For expression control of our alx-mNeonGreen construct, we inoculated 20 ml LPM with pH 7.0, 8.0 and 8.5 to an OD<sub>600</sub> of
7 KB (1,003 words) - 03:29, 2 November 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
161 KB (18,127 words) - 22:23, 1 November 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
161 KB (18,092 words) - 09:44, 23 November 2017
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...se). Here we performed saturation mutagenesis on the <i> araC </i> gene to construct AraC mutant library with four randomized ligand-binding pocket amino acid r
...sors and may not show sufficient specificity to xylulose. Hence, we aim to construct an alternative regulatory system which exploits the L-arabinose operon.
39 KB (6,105 words) - 16:49, 17 November 2017
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...2 regulated systems. Herein we will refer to each promoter + split protein construct as a “module”, i.e. a full AND-gate will require the presence and activ
...er for split T7 RNAP function. We utilised a preexisting T7 promoter (pT7) construct from the BioBrick registry, [http://parts.igem.org/Part:BBa_K1321338 K13213
33 KB (5,136 words) - 02:41, 2 November 2017
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<h3>New plan for assembling the cas13 construct</h3>
<h4>New plan for assembling the cas13a construct: Plan A and Plan B</h4>
656 KB (66,631 words) - 09:22, 15 December 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
160 KB (18,135 words) - 23:14, 1 November 2017
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...ed AcrIIA4 from G-block and amplified pSB1C3 backbone from LacILOV-mCherry Construct in pSB1C3</td>
...ILOV-mCherry construct in pKDL071 and pSB1C3 backbone from LacILOV-mCherrt construct in pSCB1C3</td>
7 KB (797 words) - 19:53, 14 December 2017
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...5 variant R19 with the PTS1* and PTS*, co-transformed with the Pex13-mRuby construct − the localization of mTurquoise tagged with PTS1* is clear and due t
...ed a Zeiss Elyra PS microscope. For Pex15 we observed localization using a construct with mVenus fused to the C-terminus of the Pex15 version we used. The fluor
59 KB (9,038 words) - 03:50, 2 November 2017
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...ngs were obtained after 24 hours of incubation. This temperature sensitive
construct was working as expected in 10β cells, elucidating higher GFP expression at
<p id="imgdescription">Figure 2: BBa_K2447014
construct which was characterised in 10β cells (incubated overnight). At higher temp
20 KB (2,876 words) - 13:25, 1 November 2017
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...work in a better understood organism and led to higher copy numbers of our
construct. </p>
<p>Our final
construct, pGUIDE, consisted of Chloramphenicol and Kanamycin resistance genes, the C
29 KB (4,511 words) - 03:55, 2 November 2017
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...">Figure 1. Graphic representation of Plant-Human genetic circuit with the construct comprised by a promoter and a terminator in opposite directions flanked by
...protein. It represents the positive control of our experiment. d) Genetic construct that allows constitutive expression of phiC31 in the plant under the contro
19 KB (2,276 words) - 03:46, 2 November 2017
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...to xylose, xylulose, ribose and mannitol. </ref> combined with a reporter construct to detect the presence of <i>C. jejuni</i>.
mtlR coding sequence was ordered as a G-block construct from IDT and was ligated into pSB1C3. As this was to be under the control o
19 KB (3,023 words) - 12:18, 12 December 2017
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...n) is added, the activity of the enzyme can be checked.<br>So far the test construct have only been implemented in silico. Also all needed primers were designed
...ed and used for a test digest with NotI. The growth of the riboswitch test construct was not properly grown. So the Golden Gate reaction and the transformation
191 KB (30,171 words) - 16:09, 15 December 2017
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...5 variant R19 with the PTS1* and PTS*, co-transformed with the Pex13-mRuby construct − the localization of mTurquoise tagged with PTS1* is clear and due t
...ed a Zeiss Elyra PS microscope. For Pex15 we observed localization using a construct with mVenus fused to the C-terminus of the Pex15 version we used. The fluor
56 KB (8,614 words) - 02:20, 2 November 2017
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...s indicate where a band with a molecular mass corresponding to that of the construct should be.
...re or during the purification. It wasn’t clear from our results why this construct was unstable, but we reasoned that it was too unstable for our use, so no f
20 KB (3,047 words) - 03:51, 2 November 2017
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...hecked our constructs for secondary structures and we scrambled the 14-3-3 construct to make sure that we get 4 completely different monomers, to make mutation
...of the 14-3-3 construct did not work, but the Gibson Assembly of the CT33 construct showed promising results.</h6>
34 KB (5,513 words) - 17:18, 1 November 2017
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...nhibit pλR, obstructing the promotion of tsPurple. Therefore, the working construct would supply us with a concentration of translated tsPurple dependant upon
...ration verified the success of ligation. Despite lacking the full, genetic construct, we managed to obtain viable data from the Chrome-Q system using the proof
21 KB (3,001 words) - 16:46, 16 December 2017
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...es each containing cells included to express recombinase and a measurement construct. These cells contain
a measurement construct with orthogonal target sites and give little fluorescence</figcaption>
12 KB (1,529 words) - 23:00, 1 November 2017
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...se the yields of anthocyanin when paired with the genes in our anthocyanin
construct. We have added the sequence to the registry for use in our composite part <
<p class="center">The Promoter we used for our Melanin
Construct is T7 promoter, This allows us to control the production of Melanin. This
14 KB (2,401 words) - 17:43, 1 November 2017
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<section><h3>1. Cloning of module 1:</h3><h4>Construct 1 --> Ptet - RBS1 - T7 RNA polymerase - terminator 1 - terminator 2.
...id not get positive results for cloning, resulting in failure to clone the construct 1.
12 KB (1,800 words) - 03:21, 2 November 2017
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<section><h3>1. Cloning of module 1:</h3><h4>Construct 1 --> Ptet - RBS1 - T7 RNA polymerase - terminator 1 - terminator 2.
...id not get positive results for cloning, resulting in failure to clone the construct 1.
12 KB (1,743 words) - 04:15, 14 December 2017
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...d to turn the protein and its required guide sequence into a BioBrick. The
construct was delivered in three fragments that had to be assembled with the backbone
...extracted from <a href="#references">Gootenberg et al. 2017</a>. This DNA
construct (7.7 kb), was ordered in three separate parts, denoted part 1 (2.5 kb)
102 KB (13,845 words) - 16:15, 15 December 2017
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...eful vector pPIGBACK for microalgae transformation. pPIGBACK is a powerful construct with NSII, AmpR, double terminator, ORI, and PrbcL, and it is able to embar
<!--construct-->
13 KB (1,734 words) - 19:39, 1 November 2017
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<h2 class="major">Construct Model</h2>
...d COX-2 (Peking iGEM Team 2015, 2015). These products are assembled into a construct composed of fusion proteins as well as split luciferase fragments (COX-2 -
78 KB (7,559 words) - 15:53, 1 November 2017
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.... Suddenly I came up with an interesting idea that what if the scaffold is construct by protein. Then I realized that scaffold is such a regular network that we
... So now the problem is how do we construct the scaffold. We knew that there was many protein self-assembly system. Usu
22 KB (3,229 words) - 09:18, 29 August 2017
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<input class="tealButton" type="button" value="Construct Design" onclick="window.location.href='#anchor3'" />
<input class="tealButton" type="button" value="Validation Construct" onclick="window.location.href='#anchor7'" />
25 KB (3,716 words) - 02:10, 2 November 2017
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...s (Center Point and Binding Partner) are connected and form the <i>Binding Construct</i>. There is a <i>Center Point</i> and at this Center Point, four Binding
<li><span>Of the Scaffold Construct</span></li>
5 KB (769 words) - 21:13, 1 November 2017
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Through simulations of our intended construct with parameters defined a priori, we were able to draw some conclusions as
<h3 class="section-heading">Control construct design</h3>
12 KB (1,643 words) - 01:46, 2 November 2017
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<a class="item passlink" data-passage="consexp">Construct Expression Vector for NzFE</a>
<td>easy to construct</td>
21 KB (2,679 words) - 13:26, 15 December 2017
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● Build up deinking enzyme construct<br>
● Transform construct into yeast<br>
12 KB (1,855 words) - 03:13, 16 December 2017
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...RNA from the HTT115 strain witouth the siRNA
construct and with the siRNA
construct (BSLA-AWD) with different hybridization oligos and treatments in a 12% no-d
<p>Figure 2. RNA from the HTT115 strain with the siRNA
construct (BSLA-SOD, BSLA-WNT and BSLA-RACI) with different hybridization oligos and
42 KB (4,985 words) - 03:59, 2 November 2017
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<td>Construct for Curvacin A expression</td>
<td>Construct for Enterocin A expression</td>
19 KB (2,225 words) - 19:40, 1 November 2017
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...e to subclone the immunity protein 2 into the tryptophan regulatory system
construct and perform proliferation tests. Unfortunately, due to time constraints, we
...uccess rate of a study utilizing machinery for genome integration. The RFP
construct was cloned into the UPO-Sevilla 2011 plasmids (<a target="_blank" href="htt
48 KB (6,966 words) - 15:28, 15 December 2017
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...contain EutSMNLK, however we were unable to obtain pure plasmid from this construct containing all microcompartment subunits as the transformed colonies became
...Tris-glycine SDS page gels of soluble and insoluble Eut S, MN, SMN and LK construct proteins. (-) indicates
12 KB (1,634 words) - 03:48, 2 November 2017
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...olor: #808080; font-size: 14px;'>Figure 1: Schematic of a generic reporter construct used to test degradation rates. Analogous constructs were built with sfGFP
...tructs (Figure 3a). Further, if you plot the gene expression speed of each construct against its degradation rate then the shape of the curve resembles that of
19 KB (2,797 words) - 03:07, 15 December 2017
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<li><b>Fer/Hyd</b>- Fer and Hyd as described above in a new biobrick construct.</li>
...i> and <i>hyd G</i>, coding all three maturation enzymes in a new biobrick construct.</li>
55 KB (9,025 words) - 13:09, 31 October 2017
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...atase that converts the enoyl-CoA into (R)-hydroxybutyrate (Lu, 2003). Our construct also includes the <i>phaC1</i> gene from <i>Pseudomonas aeruginosa</i>, enc
<h2>Genetic construct</h2>
7 KB (1,176 words) - 01:03, 2 November 2017
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...last monomer, leading to a functional valency of 3. The structure of this
construct is shown schematically in <a href="#Figure_1">Figure 1.</a>
..., leading to our second
construct, which has thus a valency of 4. The CT33
construct is schematically shown in <a href="#Figure_2">Figure 2</a> below.
7 KB (1,092 words) - 22:03, 11 December 2017
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...te-temperature cascaded system with GFP reporter. Characterisation of this construct was conducted with varying phosphate concentrations under two different tem
...led plan: Culture the transformed cells ( the temperature sensitive system construct, is transformed into MG 1655) from glycerol and incubate for 36 h in 30 deg
17 KB (2,425 words) - 16:34, 1 November 2017
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...g a VCP1-GFP-VCP2 construct and attempting to get the algae to express the construct. <br> <br>
.../2017/b/be/UConn_vcp1gfp_gel.JPG" class="vcp1-gfp"> <br> First, a VCP1-GFP construct was assembled through digesting the GFP out of its original backbone and th
15 KB (1,886 words) - 03:25, 2 November 2017
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...d series of anderson promoters next to the RNA I gene and then placed this construct next to RNA II (RNA II-Anderson-RNA I).</p>
...le copy number system. We first had to make sure that at least part of our construct is well characterized and to so we chose the rhamnose promoter from the bio
58 KB (8,538 words) - 14:52, 26 November 2017
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recombinase expression construct in pET28b, a medium copy plasmid (10~15), another one being Promoter-LoxP-T
...mediate the recombination between the two target sites on the measurement construct, excising the transcriptional terminator. This leads to the expression of R
79 KB (9,298 words) - 20:57, 1 November 2017
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The construct is long (1,029 bp), so we got only part of it sequenced due to using only o
...k sample no. 13 from the check-up digestion (Figure 8) for sequencing. The construct is long (1029 bp), so we got only
53 KB (6,877 words) - 20:55, 11 December 2017
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<cite>Figure I: Scheme of the BBa_K2425000 construct. It is important to know that the number of bases pairs is just the coding
...f METE gen (Helliwell et al.,Plant physiology, 165(1), 388-397, 2014). The construct has 574 bp and contains the 5’ UTR and the sequence of the promoter. All
17 KB (2,489 words) - 01:44, 16 December 2017
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...of the highly sensitive parameter in the design. As the original phosphate
construct <a href="http://parts.igem.org/Part:BBa_K116404">BBa_K116404</a> (
construct
5 KB (689 words) - 03:09, 2 November 2017
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.... The second step was the preparation of a pTet-GFP/RFP and a pLac-GFP/RFP construct to test these promoters in L. lactis. From next week progress for this part
...ad><tr class="tableizer-firstrow"><th>Backbone</th><th>construct A</th><th>construct B</th><th>Product name</th></tr></thead><tbody>
50 KB (7,945 words) - 03:35, 2 November 2017
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Each construct was sent for sequencing with primers VR and VF
...<li>RPA of TEV could not produce the whole (~1300 bp) construct but would produce amplicons of shorter size (100-500 bp) which was anticipa
380 KB (41,086 words) - 11:45, 1 November 2017
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Each construct was sent for sequencing with primers VR and VF
...<li>RPA of TEV could not produce the whole (~1300 bp) construct but would produce amplicons of shorter size (100-500 bp) which was anticipa
380 KB (41,105 words) - 02:27, 2 November 2017
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.../static.igem.org/mediawiki/2017/2/2b/XylRproofofconcept.jpg" alt="xylR-GFP Construct" style="width:600px;height:175px;"></center>
...nts, we eventually arrived at construct BBa_K2451013, depicted above. This construct displays promising results for m-xylene induction. The xylR coding sequence
46 KB (5,986 words) - 02:38, 2 November 2017
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..."></div><div>w that iron had no effect on the production of amilCP in this construct and we compared that to the co-transformed bacteria (plasmids 1+2). Again,
...rmed cells (i.e. cells with the FUR repressed construct and LacI repressed construct) within 24 hours, although to a lesser extent than cells which lacked the f
12 KB (1,980 words) - 18:53, 1 November 2017
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<h4>Construct 1 --> Ptet - RBS1 - T7 RNA polymerase - terminator 1 - terminator 2.</h4>
<h4>Construct 2 --> P<sub>T7</sub> - RBS2 - Chromoprotein II - RBS3 - tetR - terminator3.
11 KB (1,384 words) - 04:14, 14 December 2017
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<p>When designing this construct, we found a paper by Chen et al. that determined the optimal spacing betwee
...the production of varied gas vesicle phenotypes. Expression of the gvp3.0 construct is under control of the lac promoter (pLac), allowing inducible expression
9 KB (1,385 words) - 03:58, 2 November 2017
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...find out the best combination of vector and E.coli strain for this sensing construct.
3 KB (501 words) - 04:56, 16 December 2017
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<b>Figure 3:</b> construct design for 2 Dronpa domains
...y altering its conformation. To test this hypothesis we made the following construct:
21 KB (2,411 words) - 03:11, 16 December 2017
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...to time constraints of the parts submission deadline, we were not able to construct a new biobrick compatible hCas9 operon. We encourage next iGEM teams to pic
As mentioned earlier we were not able to construct the biobrick compatible hCas9 operon. So, the experiment described next was
25 KB (3,964 words) - 03:48, 2 November 2017
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var reservedEventNames = /^(?:change|reset|teardown|update|construct|config|init|render|complete|unrender|detach|insert|destruct|attachchild|det
....error( 'Cannot use reserved event names (change, reset, teardown, update, construct, config, init, render, unrender, complete, detach, insert, destruct, attach
1.96 MB (302,682 words) - 17:51, 24 October 2017
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...<i>Clostridium thermocellum</i>. This part could be used with any other construct to bind to cellulose.
...l peptide in form of a fusion protein accompanied with His6x-Smt3 tag. The construct can be purified with commercially available and widely used affinity chroma
18 KB (2,363 words) - 20:51, 15 December 2017
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...n later constructs and successfully isolated those. Digested all parts for construct 1 so far and gel purified the adequate bands.
Transformed cells with construct 1 and created glycerol stocks from those. Prepared a protocol for the gener
7 KB (1,157 words) - 13:03, 31 October 2017
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.... The second step was the preparation of a pTet-GFP/RFP and a pLac-GFP/RFP construct to test these promoters in L. lactis. From next week progress for this part
...ad><tr class="tableizer-firstrow"><th>Backbone</th><th>construct A</th><th>construct B</th><th>Product name</th></tr></thead><tbody>
39 KB (6,407 words) - 01:03, 2 November 2017
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...icin. Successful cloning was confirmed by gel electrophoresis, and the DNA construct was sent for sequencing. Whilst waiting for sequencing results, we began ch
...o do this, we used the already existing BioBrick BBa_I6211, containing the construct osmosensitive promoter-RBS-YFP-double terminator in the pSB1C3 backbone. As
16 KB (2,484 words) - 19:09, 15 December 2017
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...RNA from the HTT115 strain witouth the siRNA
construct and with the siRNA
construct (BSLA-AWD) with different hybridization oligos and treatments in a 12% no-d
<p>Figure 2. RNA from the HTT115 strain with the siRNA
construct (BSLA-SOD, BSLA-WNT and BSLA-RACI) with different hybridization oligos and
14 KB (1,654 words) - 01:36, 2 November 2017
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...uorescence detection assay utilizing green fluorescent protein (GFP)-fused construct to BBa_I14034 promoter part.</p>
This construct is produced by combining BBa_114034 promoter part and a GFP coding sequence
3 KB (469 words) - 03:58, 2 November 2017
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...roceeded to insert the T2A-P2A based display vector into our final ToleGen construct.
Our final experiment was to test the ToleGen construct we created <strong>(figure 5)</strong>.
20 KB (2,612 words) - 02:51, 2 November 2017
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...ow exchanging of the separate parts. As a result, we decided to divide our construct into 3 gBlocks, which could be connected to each other and placed in a pET2
...e large gBlocks and large amount of inserts, so we decided to redesign our construct, by dividing it into two gBlocks: one containing the first two monomers and
8 KB (1,272 words) - 13:50, 10 December 2017
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...xpected size band of ~3.5 kb. <strong>C:</strong> Results of <em>VanA</em> construct amplification via PCR using amplifying primers at four temperatures, 66°
...hite MoClo colonies were checked for correct insertion of the <em>KPC</em> construct via digestion with AvrII (A). All bands were of the expected size and uncut
9 KB (1,446 words) - 23:48, 1 November 2017
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<input class="tealButton" type="button" value="Validation
Construct" onclick="window.location.href='#anchor4'" />
<p class="text12j">Both the tRNA<sup>phe</sup>-MS2 aptamer
construct and MS2BP were overexpressed individually in <i>E. coli</i> BL21-Gold (DE3)
20 KB (2,975 words) - 03:38, 2 November 2017
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<p>To characterize LacILOV, cells containing LacILOV-mCherry construct were grown under a consistent light intensity and the fluorescent output wa
...tivity of the LacILOV mCherry construct, the assays for assessing reporter construct use the same hardware devices for delivery of light and dark conditions. Th
16 KB (2,418 words) - 19:50, 14 December 2017
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...ry tag has on enzyme activity. Results from this will aid how we build the construct to target the enzyme into the microcompartment at a later stage.</p>
<th>Construct</th>
26 KB (3,896 words) - 01:44, 2 November 2017
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...on developing a proof of concept for the Chrome-Q and assembling a genetic construct to use the Chrome-Q to quantify data.
Several weeks were devoted to construct pλR LacI R011 RFP in the NEB 10-beta E. coli strain, with individual parts
26 KB (3,923 words) - 17:16, 12 December 2017
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...d sequencing results from Tri-I Biotech confirmed that our final <i>pR</i> construct is correct.
...<b>Figure 2-2 Construct expressing PR. </b> Our construct includes a strong promoter, strong RBS, the <i>pR</i> ORF, and a double ter
19 KB (1,932 words) - 13:20, 31 October 2017
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<input class="tealButton" type="button" value="Validation
Construct" onclick="window.location.href='#anchor3'" />
<p class="text12j">Both the tRNA<sup>Phe</sup>-MS2
construct and MS2BP were expressed individually in <i>E. coli</i> BL21-Gold (DE3) cel
9 KB (1,447 words) - 02:13, 2 November 2017
