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...for <i>MATa</i> cleavage. After selection, by homologous recombination, we deleted the Ura-tag for further usage. We selected the target colonies(<i>SynⅩdUr ...iated during the cell fusion in yeast mating process with Terminator being deleted. </p>

...oid mate with each other. After selection, by homologous recombination, we deleted the <i>Ura-tag</i> for further usage. We selected the target colonies (<b>< ...text-align:center"><br/> Fig. 1-1. The PCR strategy for testing whether we deleted the HMRa in <b><i>SynⅩ-dUra</i></b>.

...process for MAT cleavage. After selection, by homologous recombination, we deleted the <i>Ura-tag</i> for further usage. We selected the target colonies (<b>< ...text-align:center"><br/> Fig. 1-1. The PCR strategy for testing whether we deleted the HMRa in <b><i>SynⅩ-dUra</i></b>.

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...mes involved in the expression of other quorum sensing molecules have been deleted. All these mutations make the strain JMH626 specific for detecting non-endo

...ike> <s>This is also strikethrough text, but made with s.</s> <del>This is deleted text, made with del.</del>

...contribute to the formation of curli nanofibres and will thus also remain deleted. Both pETDuet-1 and pET-28a, along with their inserts, will be transformed

chromosome to cause the gene to be replicated, inverted, displaced and deleted, gene between sites will be deleted. On the contrary when in the reverse direction,

...mes involved in the expression of other quorum sensing molecules have been deleted. All these mutations make the strain JMH626 specific for detecting non-endo

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...92;case 80:return 448;case 81:return 343;case 82:return 338;case 83:return"DELETED";case 84:return 242;case 85:return 406;case 86:return 185;case 87:return 39 ...(?:DATEDIFF\b)/i,/^(?:DECLARE\b)/i,/^(?:DEFAULT\b)/i,/^(?:DELETE\b)/i,/^(?:DELETED\b)/i,/^(?:DESC\b)/i,/^(?:DETACH\b)/i,/^(?:DISTINCT\b)/i,/^(?:DROP\b)/i,/^(?

...The <b><i>URA3</i></b> gene in the yeasts used in lab has already been deleted. Hence the loss of <i>ODCase</i> activity leads to a lack of cell growth un ...65004 (<partinfo>BBa_K2165004</partinfo>). This promoter is designed to be deleted with a few bases on the two ends. We retained 5 <i>ACE1/CUP1</i> binding si

and the natural ligand tyrosyl. For our project, we deleted the natural ligand and “Chain B”, because it was not necessary to

...y. Edited week 6 newsletter. Emailed Piktochart for reimbursement receipt. Deleted template information off of wiki pages.

<h2> iGEM Information (to be deleted as IT issues are fixed)</h2>

...y used and influence little on scoring process (with a less weight), so we deleted them and put more weight on other factors such as submission time. Driven b

<li> LADD (Aduro Biotech). Phase II. Aduro’s Live, Attenuated Double-Deleted Listeria (LADD) platform is based on proprietary attenuated strains of List

...e (ADH2) responsible for the conversion of ethanol to acetaldehyde will be deleted from strain A, hence, it will to be unable to metabolise ethanol produced a

...to perform conditional gene knockout, where a gene can be deleted in specific time and

...e (ADH2) responsible for the conversion of ethanol to acetaldehyde will be deleted from the first subpopulation, hence, it will to be able to metabolise ethan

...e activity, because the protein domain conferring endonuclease activity is deleted from the full Cas9 protein. Thus, dCas9, cannot break double-stranded DNA,

...ontain the same size of insert and vector and nothing has been inserted or deleted during dimerisation. The lower gel shows the test devices uncut, and shows

...nt variants. We made variants where the leucine zipper attached to T18 was deleted (pT8O-LZ stop; BBa_K2389030), the leucine zipper was mutated to abolish dim

...t G-protein Ste2 with the human sweet receptor T1R2-T1R3, additionally, we deleted some genes to further improve the sensitivity of the T1R2-T1R3 reporter sig

...standard, meaning that several prohibited restriction sequences have been deleted by silent mutations. Furthermore, we have lowered the GC content of the pro

...brane helices, the C-terminus and the 5 nearest transmembrane helices were deleted, resulting in the increase in Na+/K+

...could either be substituted by one of the four natural bases or completely deleted. There is a sgRNA for all five cases that binds the sequence recruiting Cas

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// List of deleted data cache ids, so we can reuse them

IMU051[3] strain (strain A) has been deleted for the following genes: malΔ(mal11-mal12::loxP, mal21-mal22::loxP, mal31-

...es, the C-terminus and the 5 nearest transmembrane helices of AtNHX1 where deleted, resulting in the increase in Na+/K+

...e activity, because the protein domain conferring endonuclease activity is deleted from the full Cas9 protein. Thus, dCas9, cannot break double-stranded DNA,

...mology directed repair with our designed repair template, <i>cod</i>A gets deleted.

...as expressed in the Strains BY4742 and BY4742 with the gene <em>Pex11</em> deleted. <br>

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...ab to give us 2 <i>V. harveyi</i> strains: a WT one, and the JMH626 strain deleted from the gene CqsA, which can&rsquo;t produce C8-CAI-1 by itself. We&rsquo;

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...o that it is no longer toxic. In E. coli when the crcB gene is genetically deleted (ΔcrcB), the phenotype is increased sensitivity to fluoride and concentrat

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...ture above, the white colonies were inserted with the HrtR and the RFP was deleted. The red one is that the RFP still exists because HrtR is not inserted norm

Table 1. The clpXP genes in these strains were deleted to obtain more robust oscillations.<br>

...i = Dps-type ferritin, ftn = archetypal ferritin, bfr M52L and M52H = heme-deleted bacterioferritins<br>

...eroxidase functions similarly to Orp1, but the resolving cysteine has been deleted to promote the transfer of sulfenic acid to the fluorescent protein. Simila

...nbsp;We combined the fungal diseases data and the weather information then deleted the diseases data that had no weather data to match with. Later, these data

...hat corresponded to the data forward. Then, we combined these two and then deleted the recorded data that had no weather data to match with.

...o that it is no longer toxic. In E. coli when the crcB gene is genetically deleted (ΔcrcB), the phenotype is increased sensitivity to fluoride and concentrat

...ase, making Bacillus more resistant to several beta-lactam antibiotics. We deleted this gene to make the biosensor more sensitive to the antibiotic compounds

...e activity, because the protein domain conferring endonuclease activity is deleted from the full Cas9 protein. Thus, dCas9, cannot break double-stranded DNA,

<span class="bold">5. Editing the circuit:</span> We deleted some extra parts of the ETH_Zurich device because we decided to use only th

gene which encodes for the TCA cycle enzyme fumarase deleted from its genome. This is a fully segregated strain that has been made clean

<span class="bold">5. Editing the circuit:</span> We deleted some extra parts of the ETH_Zurich device because we decided to use only th

...carefully collated and analyzed our instructors’ suggestion. Finally, we deleted the last two parts and started to focus on using reconstructive intestinal

...carefully collated and analyzed our instructors’ suggestion. Finally, we deleted the last two parts and started to focus on using reconstructive intestinal

gene in between the loxP sites while the gene from which it was deleted just contains a loxP site.

...of a different genus (e.g. it only has 'self' genes inserted, rearranged, deleted etc.) then its status is unclear at the moment, since the EU has still not

...:"The template does not contain the TextBox widget",E1011:'Items cannot be deleted from the List. Implement the "remove" function in the data store',E1012:"Ed

<p>For this part, we have deleted the HNH domain, Rec2 domain and RuvCIII-2 domain of the SP-dCas9. The tripl

...nt variants. We made variants where the leucine zipper attached to T18 was deleted (pT8O-LZ stop; BBa_K2389030), the leucine zipper was mutated to abolish dim

...it is no longer toxic. In <i>E. coli</i> when the crcB gene is genetically deleted (ΔcrcB), the phenotype is increased sensitivity to fluoride and concentrat

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;First , we deleted the peptides which amino acid lengths were longer than one hundred amino ac

<p>We redesigned the part sequence provided by iGEM16_Washington. We deleted irrelevant bases on the two ends of this promoter and retained the core seq

...ncations were highlighted. Note the space left behind when the domains are deleted. While the truncations in this project are designed to minimally impact sec

...eroxidase functions similarly to Orp1, but the resolving cysteine has been deleted to promote the transfer of sulfenic acid to the fluorescent protein. Simila

...plasmid loss. In the condition, antibiotic resistance marker can be deleted for biosafety, and the selection

...<p>We directly replaced the 6His tag with FBPA without being deleted its stop codon to make a C-fusion protein. So that the whole part can only

...o that it is no longer toxic. In E. coli when the crcB gene is genetically deleted (ΔcrcB), the phenotype is increased sensitivity to fluoride and concentrat

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..."http://parts.igem.org/Part:BBa_K2389030">pT8O-LZ stop: T18 leucine zipper deleted </a> </li>

...nly the upstream and downstream homologous regions flanking the gene to be deleted / introduced, and another that contains an extra selection cassette in the

...nd the TolR-sequence. TolR is inserted at bp 74 of pET-DUET, bp 74-168 are deleted from the backbone where TolR is inserted.</p>

...idues to the stabilization of the α-helix. When either G58 or T60-T61 was deleted from the LacILOV sequence, I-TASSER predicted a disrupted alpha-helix. Inte

CHOP and ∆crcB (<i>E. coli</i> with the Fluoride Channel deleted) were added to 50µg/mL chloramphenicol plates and observed over the course

...o that it is no longer toxic. In E. coli when the crcB gene is genetically deleted (ΔcrcB), the phenotype is increased sensitivity to fluoride and concentrat

...as expressed in the Strains BY4742 and BY4742 with the gene <em>Pex11</em> deleted. <br>

...nly the upstream and downstream homologous regions flanking the gene to be deleted / introduced, and another that contains an extra selection cassette in the

...nly the upstream and downstream homologous regions flanking the gene to be deleted / introduced, and another that contains an extra selection cassette in the

...nly the upstream and downstream homologous regions flanking the gene to be deleted / introduced, and another that contains an extra selection cassette in the