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...>Testing the performance of phiC31 recombinase using the register assembly construct with the attachment sites PxB.</p>
...tutive expression of phiC31 in the plant. c) Graphic representation of the construct comprised by a promoter and a terminator in opposite directions flanked by
50 KB (6,682 words) - 03:33, 2 November 2017
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<br> (2) Endolysin construct finish
<br> (5) Backbone construct-3NSII, 5NSII,AMP, B0015, and ORI (PCR: dream taq)<br>
26 KB (3,130 words) - 16:14, 28 November 2017
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...rror, so instead we performed 2-step NEB Phusion HF PCR of pSB1C3 with RFP construct</p>
<li>Performed Phusion PCR of pSB1C3 with RFP construct using psB1C3 #2 and the pKDL071 FWD/RVS primers
76 KB (12,349 words) - 20:03, 14 December 2017
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...ycin. The enzyme of this step is BsmBI again. This level is useful, if the construct you are designing requires multiple genes to be transformed into one yeast
...Cross <i>et al.</i>, published in 2017, in which they present a synthetic construct of the Pex5 protein, partly <i>Arabidopsis thaliana</i> and partly <i>Physc
199 KB (29,417 words) - 03:20, 2 November 2017
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...ldenBraid multipartite assembly reaction was performed to create a genetic construct composed by the transcriptional factor, PIF6, fused to a DNA-binding domain
...ression along time for different treatments, including the light-inducible construct (Etr8) under white-light conditions, and a weak constitutive promoter and a
51 KB (6,067 words) - 19:12, 14 December 2017
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.... The construct used for cutting <i>STE2</i> is shown in Figure 2, and the construct used for cutting <i>STE3</i> in Figure 3.
<figcaption><b>Figure 2.</b> gRNA construct for deletion of <i>STE2</i>.
253 KB (35,104 words) - 15:59, 30 October 2017
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<li style="font-family: Rubik">Designed, and began to construct, a variety of framework compatible systems, including a synthetic promoter
...moter was added to enable expression to be under the control of IPTG. This construct was then submitted to IDT for synthesis as a gBlock.
184 KB (26,744 words) - 19:53, 1 November 2017
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...moter was added to enable expression to be under the control of IPTG. This
construct was then submitted to IDT for synthesis as a gBlock.
...replacing the IPTG sensing system in the original detector module with the
construct detailed above, creating part <a href="http://parts.igem.org/Part:BBa_K2205
185 KB (26,866 words) - 22:39, 1 November 2017
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...subject, and also they get information about yeast extraction and protein construct.",,,,Consulted experts to gain more information on cyanobacteria and mathem
...ext two exchanges mainly focused on cooperation</li><li> Help BNU-China to construct a part of the gene circuit, and meanwhile, BNU-China helps with the charact
1.09 MB (163,642 words) - 17:36, 30 October 2017
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<p>Assess robustness of a GFP reporter construct in a range of environments.</p>
...ly. The Golden Gate assembly method successfully produced the GFP reporter construct, however it involved more steps and was more time consuming than Gibson ass
20 KB (2,994 words) - 21:41, 1 November 2017
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...ed split-luciferase system characterized by the 2015 Peking iGEM team, our construct consists of a promoter reporter system that looks at two downstream product
...ia in their intestines are influencing on protein nutrition. We thought to construct a pathway to synthesize amino acids from nitrogen in E.coli, introduce it i
400 KB (63,299 words) - 15:37, 15 August 2018
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...(Kittleson, 2012), which was essential both to obtain P1 particles and to construct each complete CRISPR system.</p>
engineered phagemid would need to become a P1 particle and construct whichever CRISPR system we have inserted into it within an <em>E. coli</em>
25 KB (3,973 words) - 20:43, 25 November 2017
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...lls (control) (15min) or not mixed in the presence or absence of the EL222 construct. Cell mixtures would have then be passed through a particle sizer with a ra
...fluorescence microscopy. We also included a His-Tag within all SpyCatcher construct variants to enable in-vitro analysis of complexes formed with SpyTag varian
122 KB (15,402 words) - 02:31, 2 November 2017
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<td class="col0"><strong>Construct</strong></td><td class="col1">6.43 µg</td>
<td class="col0"><strong>Construct</strong></td><td class="col1">6.43 µg</td>
167 KB (25,036 words) - 21:56, 31 October 2017
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Construct standard structure of integrase int5 and int8.<br>
Construct the expression system of integrase TP901, int2, int3, int4, int5, int7, int
16 KB (1,672 words) - 13:45, 1 November 2017
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...ycin. The enzyme of this step is BsmBI again. This level is useful, if the construct you are designing requires multiple genes to be transformed into one yeast
...Baker <i>et al.</i>, published in 2017, in which they present a synthetic construct of the Pex5 protein, partly <i>Arabidopsis thaliana</i> and partly <i>Physc
105 KB (15,409 words) - 02:56, 2 November 2017
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...o take our chances by building a D.I.V.E.R.T. cassette as well as a helper construct (carrying the RT, FLP and the primer cassettes) according to the best of ou
...synthetic regulatory elements<sup>1–3</sup> to keep the synthesized construct short. The synthetic galactose-inducible minimal promoter we used (sequence
68 KB (10,146 words) - 17:37, 15 December 2017
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...ctroporated DNA (BRED) (Figure 1). In this technique a double stranded DNA construct containing the
...to be replaced. The host cell for recombineering of the phage and the DNA construct contains a plasmid conferring genes promoting recombination. The double-str
10 KB (1,666 words) - 23:47, 1 November 2017
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...ycin. The enzyme of this step is BsmBI again. This level is useful, if the construct you are designing requires multiple genes to be transformed into one yeast
...Baker <i>et al.</i>, published in 2017, in which they present a synthetic construct of the Pex5 protein, partly <i>Arabidopsis thaliana</i> and partly <i>Physc
98 KB (14,470 words) - 00:02, 2 November 2017
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<a href="#ConDPR" class="pageNavSm">
Construct Design</a>
<a href="#ConDBio" class="pageNavSm">
Construct Design</a>
55 KB (6,458 words) - 14:08, 1 November 2017
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<td>Simple, easiest to construct</td>
...espective promoter (PphlF), and a GFP reporter (BBa_K2477008). We grew our construct in different molarities of fusaric acid (0µM, 100µM, and 500 
232 KB (33,473 words) - 02:45, 2 November 2017
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<div class="hull-content">Our dCas13a - GFP construct was synthesized by Integrated DNA Technologies. The dCas13a gene sequence w
To improve imaging, the dCas13a - GFP construct was sequesteredTo reduce background noise, unbound dCas13a - GFP should be
24 KB (3,114 words) - 00:16, 2 November 2017
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construct.<br><br>
<li>Construct half of the flip-flop structure and express it in <i>E. coli.</i></li>
170 KB (16,709 words) - 06:32, 15 December 2017
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<div class="hull-content">Our dCas13a - GFP construct was synthesized by Integrated DNA Technologies. The dCas13a gene sequence w
To improve imaging, the dCas13a - GFP construct was sequesteredTo reduce background noise, unbound dCas13a - GFP should be
24 KB (3,037 words) - 23:41, 15 December 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
159 KB (17,948 words) - 20:12, 1 November 2017
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<h2 class="textheading1" style="float:center"><u> Full Construct Testing</u> </h2>
...of GFP, the data seemed to be a bit problematic. We took a few of our full construct colonies as well as a few wild type Kanamycin resistant colonies and we ino
12 KB (1,480 words) - 02:11, 2 November 2017
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...This figure shows restriction enzyme check electrophoresis result of NrtA construct.
<h4>Endolysin Construct</h4>
29 KB (4,011 words) - 19:07, 1 November 2017
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...licated many times and matured, this allows us to have more cells with the
construct that can produce more pigment faster.
...wasted on transcribing an overly-long mRNA transcript. The melA expression
construct is the improved part that we were able to characterize the most (see <a hre
12 KB (1,924 words) - 17:29, 1 November 2017
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...luorescence was measured 20 minutes after this pH shift. The asr-mCardinal construct was inducible by change from pH 7.0 to pH 5.0 and lower but no fluorescence
...s in LPM with pH 7 overnight. For expression control of our alx-mNeonGreen construct, we inoculated 20 ml LPM with pH 7.0, 8.0 and 8.5 to an OD<sub>600</sub> of
7 KB (1,003 words) - 03:29, 2 November 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
161 KB (18,127 words) - 22:23, 1 November 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
161 KB (18,092 words) - 09:44, 23 November 2017
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...se). Here we performed saturation mutagenesis on the <i> araC </i> gene to construct AraC mutant library with four randomized ligand-binding pocket amino acid r
...sors and may not show sufficient specificity to xylulose. Hence, we aim to construct an alternative regulatory system which exploits the L-arabinose operon.
39 KB (6,105 words) - 16:49, 17 November 2017
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...2 regulated systems. Herein we will refer to each promoter + split protein construct as a “module”, i.e. a full AND-gate will require the presence and activ
...er for split T7 RNAP function. We utilised a preexisting T7 promoter (pT7) construct from the BioBrick registry, [http://parts.igem.org/Part:BBa_K1321338 K13213
33 KB (5,136 words) - 02:41, 2 November 2017
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<h3>New plan for assembling the cas13 construct</h3>
<h4>New plan for assembling the cas13a construct: Plan A and Plan B</h4>
656 KB (66,631 words) - 09:22, 15 December 2017
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Waiting on sequencing primers for my construct
Ordered Cph8 construct partly gene, partly gblock
160 KB (18,135 words) - 23:14, 1 November 2017
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...ed AcrIIA4 from G-block and amplified pSB1C3 backbone from LacILOV-mCherry Construct in pSB1C3</td>
...ILOV-mCherry construct in pKDL071 and pSB1C3 backbone from LacILOV-mCherrt construct in pSCB1C3</td>
7 KB (797 words) - 19:53, 14 December 2017
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...5 variant R19 with the PTS1* and PTS*, co-transformed with the Pex13-mRuby construct − the localization of mTurquoise tagged with PTS1* is clear and due t
...ed a Zeiss Elyra PS microscope. For Pex15 we observed localization using a construct with mVenus fused to the C-terminus of the Pex15 version we used. The fluor
59 KB (9,038 words) - 03:50, 2 November 2017
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...ngs were obtained after 24 hours of incubation. This temperature sensitive
construct was working as expected in 10β cells, elucidating higher GFP expression at
<p id="imgdescription">Figure 2: BBa_K2447014
construct which was characterised in 10β cells (incubated overnight). At higher temp
20 KB (2,876 words) - 13:25, 1 November 2017
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...work in a better understood organism and led to higher copy numbers of our
construct. </p>
<p>Our final
construct, pGUIDE, consisted of Chloramphenicol and Kanamycin resistance genes, the C
29 KB (4,511 words) - 03:55, 2 November 2017
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...">Figure 1. Graphic representation of Plant-Human genetic circuit with the construct comprised by a promoter and a terminator in opposite directions flanked by
...protein. It represents the positive control of our experiment. d) Genetic construct that allows constitutive expression of phiC31 in the plant under the contro
19 KB (2,276 words) - 03:46, 2 November 2017
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...to xylose, xylulose, ribose and mannitol. </ref> combined with a reporter construct to detect the presence of <i>C. jejuni</i>.
mtlR coding sequence was ordered as a G-block construct from IDT and was ligated into pSB1C3. As this was to be under the control o
19 KB (3,023 words) - 12:18, 12 December 2017
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...n) is added, the activity of the enzyme can be checked.<br>So far the test construct have only been implemented in silico. Also all needed primers were designed
...ed and used for a test digest with NotI. The growth of the riboswitch test construct was not properly grown. So the Golden Gate reaction and the transformation
191 KB (30,171 words) - 16:09, 15 December 2017
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...5 variant R19 with the PTS1* and PTS*, co-transformed with the Pex13-mRuby construct − the localization of mTurquoise tagged with PTS1* is clear and due t
...ed a Zeiss Elyra PS microscope. For Pex15 we observed localization using a construct with mVenus fused to the C-terminus of the Pex15 version we used. The fluor
56 KB (8,614 words) - 02:20, 2 November 2017
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...s indicate where a band with a molecular mass corresponding to that of the construct should be.
...re or during the purification. It wasn’t clear from our results why this construct was unstable, but we reasoned that it was too unstable for our use, so no f
20 KB (3,047 words) - 03:51, 2 November 2017
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...hecked our constructs for secondary structures and we scrambled the 14-3-3 construct to make sure that we get 4 completely different monomers, to make mutation
...of the 14-3-3 construct did not work, but the Gibson Assembly of the CT33 construct showed promising results.</h6>
34 KB (5,513 words) - 17:18, 1 November 2017
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...nhibit pλR, obstructing the promotion of tsPurple. Therefore, the working construct would supply us with a concentration of translated tsPurple dependant upon
...ration verified the success of ligation. Despite lacking the full, genetic construct, we managed to obtain viable data from the Chrome-Q system using the proof
21 KB (3,001 words) - 16:46, 16 December 2017
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...es each containing cells included to express recombinase and a measurement construct. These cells contain
a measurement construct with orthogonal target sites and give little fluorescence</figcaption>
12 KB (1,529 words) - 23:00, 1 November 2017
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...se the yields of anthocyanin when paired with the genes in our anthocyanin
construct. We have added the sequence to the registry for use in our composite part <
<p class="center">The Promoter we used for our Melanin
Construct is T7 promoter, This allows us to control the production of Melanin. This
14 KB (2,401 words) - 17:43, 1 November 2017
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<section><h3>1. Cloning of module 1:</h3><h4>Construct 1 --> Ptet - RBS1 - T7 RNA polymerase - terminator 1 - terminator 2.
...id not get positive results for cloning, resulting in failure to clone the construct 1.
12 KB (1,800 words) - 03:21, 2 November 2017
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<section><h3>1. Cloning of module 1:</h3><h4>Construct 1 --> Ptet - RBS1 - T7 RNA polymerase - terminator 1 - terminator 2.
...id not get positive results for cloning, resulting in failure to clone the construct 1.
12 KB (1,743 words) - 04:15, 14 December 2017
