Difference between revisions of "Team:Bielefeld-CeBiTec"

(changed the way css is embedded)
 
(46 intermediate revisions by 3 users not shown)
Line 1: Line 1:
−
{{Bielefeld-CeBiTec}}
+
{{Team:Bielefeld-CeBiTec/Head}}
−
 
+
{{Team:Bielefeld-CeBiTec/Header}}
 
<html>
 
<html>
−
<head>
+
<body>
−
<link rel="stylesheet" type="text/css"
+
−
href="https://2017.igem.org/Template:Team:Bielefeld-CeBiTec/CSS?action=raw&ctype=text/css" />
+
−
</head>
+
−
<body>
+
−
<!---
+
−
<div class="column full_size" >
+
−
<img src="http://placehold.it/2000x300/d3d3d3/f2f2f2">
+
−
</div>
+
−
--->
+
  
−
<div class="column full_size" >
+
 
−
<div id="main">
+
 
−
<article> With their broad chemical and functional diversity ncAAs provide a variety of promising applications including proteinlabeling, photocaging, structure analysis and specific protein interactions. Therefore, the development of a new iGEM toolkit for the translational incorporation of non-canonical amino acids (ncAAs) in E. coli would be a great contribution to the iGEM community. The site-specific incorporation of ncAAs requires the directed evolution of tRNA and aminoacyl tRNA synthetase pairs, which in turn mediate the introduction of a ncAA for a certain codon. We are especially exploring the application of unnatural base pairs (UBPs) as an expansion of the genetic code. This approach promises an optimal orthogonality to the autologous translation apparatus and a high flexibility concerning the incorporation of multiple ncAAs. We will utilize several systems to achieve a high retention efficiency. As examples for the successful incorporation of non-canonical amino acids we will develop a rapid test for prions and a new chromatography method for a mild elution of proteins.</article>
+
<!-- The content of this page -->
−
<p>For more information visit our <b><a href="https://www.researchgate.net/publication/318084694_Expansion_of_the_genetic_code_for_the_translational_incorporation_of_non-canonical_amino_acids">project poster</a></b> on reasearch gate</p>
+
<div id="chart">
−
<br>
+
<script src="https://d3js.org/d3.v4.min.js"></script>
−
<br>
+
<script>
−
<p> Our wiki is at the moment under construction, but you can follow our social media to be up to date! </p>
+
 
−
<p><a href="https://twitter.com/iGEM_Bielefeld">Twitter</a>
+
        function rand (min, max) {return Math.floor(Math.random() * (max - min + 1)) + min;}
−
<a href="https://www.facebook.com/igembielefeld/">Facebook</a>
+
 
−
<a href="https://www.instagram.com/igem.bielefeld/">Instagram</a></p>
+
        var svg = d3.select("#chart")
−
<br>
+
    .append("svg")
−
<p> You can also contact us per email for collaborations and more! :) </p>
+
    .attr("width", 1580)
−
<p> <a href="&#109;&#097;&#105;&#108;&#116;&#111;&#058;&#105;&#110;&#102;&#111;&#064;&#105;&#103;&#101;&#109;&#045;&#098;&#105;&#101;&#108;&#101;&#102;&#101;&#108;&#100;&#046;&#100;&#101;"> info[@]igem-bielefeld.de </a></p>
+
      .attr("height", 500);
 +
 
 +
        var ranobj = "";
 +
        var rancol = "";
 +
 
 +
 
 +
        for(var i = 0; i < 200; i++){ranobj = ranobj + "" + rand(2, 2) + "";};
 +
 
 +
 
 +
        var rancol1 = function(x){
 +
 
 +
            if(x === 1){return "#E6E6E6";}
 +
 
 +
            else if(x === 2){return "#bababa";}
 +
 
 +
            else if(x === 3){return "#8b8b8b";}
 +
 
 +
            else if(x === 4){return "#C03178";}
 +
 
 +
            else if(x === 5){return "#a48bec";}
 +
 
 +
            else if(x === 6){return "#8bc895";}
 +
 
 +
            else if(x === 7){return "#D83EFF";}
 +
 
 +
            else if(x === 8){return "#A02965";}
 +
 
 +
            else if(x === 9){return "#3189c0";}
 +
 
 +
            else if(x === 10){return "#59d4d4";}
 +
 
 +
            else if(x === 11){return "#ff6a79";}
 +
 
 +
            else if(x === 12){return "#adff29";}
 +
 
 +
        };
 +
 
 +
        var rancol2 = function(x){
 +
 
 +
            if(x === 1){return "#666666";}
 +
 
 +
            else if(x === 2){return "#808080";}
 +
 
 +
            else if(x === 3){return "#666666";}
 +
 
 +
            else if(x === 4){return "#6B1B43";}
 +
 
 +
            else if(x === 5){return "#7170fe";}
 +
 
 +
            else if(x === 6){return "#42b45a";}
 +
 
 +
            else if(x === 7){return "#6C0056";}
 +
 
 +
            else if(x === 8){return "#5B1739";}
 +
 
 +
            else if(x === 9){return "#236189";}
 +
 
 +
            else if(x === 10){return "#3e9c92";}
 +
 
 +
            else if(x === 11){return "#cb3f51";}
 +
 
 +
            else if(x === 12){return "#07de00";}
 +
 
 +
        };
 +
 
 +
        for(var j = 0; j < ranobj.length; j++){
 +
 
 +
            var randy = rand(1,12);
 +
 
 +
            if(ranobj.charAt(j) === "1"){
 +
 
 +
                svg.append("circle")
 +
 
 +
                  .attr("cx", function(){return rand(0, 1600);})
 +
 
 +
                  .attr("cy", function(){return rand(0, 800);})
 +
 
 +
                  .attr("r", function(){return rand(5, 100);})
 +
 
 +
                  .style("stroke", function(){return rancol2(randy);})
 +
 
 +
                  .style("stroke-width", 5)
 +
 
 +
                  .style("fill", function(){return rancol1(randy);});;}
 +
 
 +
            else if(ranobj.charAt(j) === "2"){
 +
 
 +
                var circ = svg.append("circle")
 +
 
 +
                  .attr("cx", function(){return rand(0, 1600);})
 +
 
 +
                  .attr("cy", function(){return rand(0, 800);})
 +
 
 +
                  .attr("r", rand(30, 50))
 +
 
 +
                  .style("stroke", function(){return rancol2(randy);})
 +
 
 +
                  .style("stroke-width", 5)
 +
 
 +
                  .style("fill", function(){return rancol1(randy);});
 +
 
 +
                    circ.append("animate")
 +
 
 +
                            .attr("attributeName", "cx")
 +
 
 +
                            .attr("attributeType", "XML")
 +
 
 +
                            .attr("values", function(){
 +
 
 +
                                var ranheight = "";
 +
 
 +
                                for(var k = 0; k < 1; k++){ranheight = ranheight + "" + ((rand(-100, 1800) + rand(-100, 1800) + rand(-100, 1800)) / 3) + "" + ";";};
 +
 
 +
                                return ranheight;})
 +
 
 +
                            .attr("begin", "0s")
 +
 
 +
                            .attr("dur",function(){return "" + rand(2, 4) + "s"})
 +
 
 +
                            .attr("repeatCount", "indefinite");
 +
 
 +
                    circ.append("animate")
 +
 
 +
                            .attr("attributeName", "cy")
 +
 
 +
                            .attr("attributeType", "XML")
 +
 
 +
                            .attr("values", function(){
 +
 
 +
                                var ranheight = "";
 +
 
 +
                                for(var k = 0; k < 50; k++){ranheight = ranheight + "" + rand(-100, 900) + "" + ";";};
 +
 
 +
                                return ranheight;})
 +
 
 +
                            .attr("begin", "0s")
 +
 
 +
                            .attr("dur", function(){return "" + rand(200, 400) + "s"})
 +
 
 +
                            .attr("repeatCount", "indefinite");}
 +
   
 +
 
 +
            else if(ranobj.charAt(j) === "3"){
 +
 
 +
                svg.append("ellipse")
 +
 
 +
                  .attr("cx", function(){return rand(0, 2000);})
 +
 
 +
                  .attr("cy", function(){return rand(0, 2000);})
 +
 
 +
                  .attr("rx", function(){return rand(5, 100);})
 +
 
 +
                  .attr("ry", function(){return rand(5, 100);})
 +
 
 +
                  .style("stroke", rancol(rand(1,2)))
 +
 
 +
                  .style("stroke-width", function(){return rand(1, 10);})
 +
 
 +
                  .style("fill", rancol(rand(1,2)));}}
 +
 
 +
 
 +
    svg.append("svg:image")
 +
.attr("xlink:href", "https://static.igem.org/mediawiki/2017/4/4a/T--Bielefeld-CeBiTec--expand_monochrome_white_2.svg")
 +
.attr("width", 1000)
 +
.attr("height", 600)
 +
.attr("x", 400)
 +
.attr("y",-50);
 +
 +
var width = window.innerWidth;
 +
 +
$offset = width - 1600;
 +
 +
if (width > 1600) {
 +
$offset = 0
 +
}
 +
 +
$("svg").css({top: 0, left: $offset, position:'relative'});
 +
 +
$("svg").attr("width", 2000);
 +
 +
 
 +
      </script>
 +
  </div>
 +
 
 +
<div class="container start-page">
 +
 
 +
<!-- Screen 1 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/7/73/T--Bielefeld-CeBiTec--start-page-screen1.png">
 +
</div>
 +
<!-- Screen 2 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/c/c4/T--Bielefeld-CeBiTec--start-page-screen2.png">
 +
</div>
 +
<!-- Screen 3 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/a/ad/T--Bielefeld-CeBiTec--start-page-screen3.png">
 +
</div>
 +
<!-- Screen 4 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/1/13/T--Bielefeld-CeBiTec--start-page-screen4.png">
 +
</div>
 +
<!-- Screen 5 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/0/01/T--Bielefeld-CeBiTec--start-page-screen5.png">
 +
</div>
 +
<!-- Screen 6 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/3/3b/T--Bielefeld-CeBiTec--start-page-screen6.png">
 +
</div>
 +
<!-- Screen 7 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/8/81/T--Bielefeld-CeBiTec--start-page-screen7.png">
 +
</div>
 +
<!-- Screen 8 -->
 +
<div class="start-page-screen">
 +
<img class="start-page-image" src="https://static.igem.org/mediawiki/2017/b/b4/T--Bielefeld-CeBiTec--start-page-screen8.png">
 
</div>
 
</div>
−
</div>
 
−
</body>
 
−
</html>
 
  
 +
<div class="contentbox" style="background: #efefef">
 +
<div class="content">
 +
<h2>Achievements</h2>
 +
<div class="article">
 +
The following list sums up all of our achievements and refers to pages of this wiki containing detailed information. Next to this we recorded the <b><a href="https://youtu.be/1f5vwcrvUac">original presentation</a></b> of our project in Boston at the Giant Jamboree, which sums up and explains the project in less than 20 minutes:
 +
</div>
 +
<iframe width="560" height="315" style="margin: auto; display: block; margin-top: 25px; margin-bottom: 25px;;" src="https://www.youtube.com/embed/1f5vwcrvUac" frameborder="0" gesture="media" allow="encrypted-media" allowfullscreen></iframe>
 +
<div lass="article">
 +
<h3>Awards</h3>
 +
<ul style="margin-left: 5%;">
 +
<li>Best Foundational Advance Project</li>
 +
<li>Best New Basic Part</li>
 +
</ul>
 +
<h3>Nominations</h3>
 +
<ul style="margin-left: 5%;">
 +
<li>Best Software</li>
 +
<li>Best Hardware</li>
 +
<li>Best New Composite Part</li>
 +
<li>Best Part Collection</li>
 +
</ul>
 +
</div>
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br>
 +
<b>We established two orthogonal methods for the detection of unnatural base pairs in a target sequence: an <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Software">Oxford Nanopore sequencing</a> application and an enzyme based detection method</b>
 +
</article>
 +
</div>
 +
</div>
  
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b>Development of a <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Software">software</a> suite for these orthogonal methods </b>
 +
</article>
 +
</div>
 +
</div>
 +
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br>
 +
<b>Integration and characterization of  the <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/unnatural_base_pair/uptake_and_biosynthesis">nucleotide transporter PtNTT2</a> from <i>P.tricornutum</i> in <i>E.coli</i> for the uptake of  unnatural nucleoside triphosphates</b>
 +
</article>
 +
</div>
 +
</div>
 +
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b>Confirmation that certain Taq DNA polymerases can efficiently <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/unnatural_base_pair/preservation_system">incorporate unnatural nucleotides</a> </b>
 +
</article>
 +
</div>
 +
</div>
 +
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b>Construction of a <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox">toolbox</a> consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids</b>
 +
</article>
 +
</div>
 +
</div>
 +
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b><a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox/labeling">Colocalization</a> of the RuBisCo and and subcellular compartment (carboxysome) using a fluorescent amino acid</b>
 +
</article>
 +
</div>
 +
</div>         
 +
           
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b>Development of a <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox/photoswitching">photoswitchable lycopene pathway</a></b>
 +
</article>
 +
</div>
 +
</div>
 +
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br>
 +
<b>Design, <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/toolbox/fusing">chemical synthesis</a> and proof of functionality of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine</b>
 +
</article>
 +
</div>
 +
</div>
 +
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b><a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Model">Modeling</a> more than ten new aaRS sequences</b>
 +
</article>
 +
</div>
 +
</div>
 +
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b><a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/translational_system/library_and_selection">Library development</a> with several hundred thousand sequences for selecting aminoacyl-tRNA synthetases</b>
 +
</article>
 +
</div>
 +
</div>
 +
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br>
 +
<b>Construction of positive and negative <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Results/translational_system/library_and_selection">selection plasmids</a> for the evolution of new synthetases for non-canonical amino acids</b>
 +
</article>
 +
</div>
 +
</div>
 +
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b><a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Improve">Improvement</a> of an aminoacyl-tRNA synthetase test-system by introducing a FRET-system and development of a ranking system</b>
 +
</article>
 +
</div>
 +
</div>
 +
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b>Construction of an <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Hardware">LED panel</a> for irradiating 96-well microtiter plates, which can be used to manipulate non-canonical amino acids and for other applications  </b>
 +
</article>
 +
</div>
 +
</div>
 +
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b>Development of an <a target="_blank" href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Software">Android</a> App to control the LED panel with your smartphone via Bluetooth</b>
 +
</article>
 +
</div>
 +
</div>
 +
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br>
 +
<b>Writing a <a target="_blank" href="https://static.igem.org/mediawiki/2017/1/1e/T--Bielefeld-CeBiTec--DKE_Biosafety_Report.pdf">biosafety report</a> entitled “Auxotrophy to Xeno-DNA: A Comprehensive Exploration of Combinatorial Mechanisms for a High-Fidelity Biosafety System” </b>
 +
</article>
 +
</div>
 +
</div>
 +
 +
<div class="contentline">
 +
<div class="third" style="width: 15%; margin-left: 5%;">
 +
<div class="figure large">
 +
<img class="figure image" src="https://static.igem.org/mediawiki/2017/9/94/T--Bielefeld-CeBiTec--YKE_Bingo.png">
 +
<p class="figure subtitle"></p>
 +
</div>
 +
</div>
 +
<div class="third double">
 +
<article><br><br>
 +
<b>Writing the <a target="_blank" href="https://static.igem.org/mediawiki/2017/1/18/T--Bielefeld-CeBiTec--CMZ-ChImp.pdf">ChImp Report</a> on “Chances and Implications of an Expanded Genetic Code”</b>
 +
</article>
 +
</div>
 +
</div>
 +
<div class="bevel bl"></div>
 +
    </div>
 +
</div>
 +
 +
 +
</body>
 +
<script>
 +
$("#home").addClass("active");
 +
</script>
 +
</html>
 
{{Team:Bielefeld-CeBiTec/Footer}}
 
{{Team:Bielefeld-CeBiTec/Footer}}
 +
<br>

Latest revision as of 15:18, 16 December 2017

Achievements

The following list sums up all of our achievements and refers to pages of this wiki containing detailed information. Next to this we recorded the original presentation of our project in Boston at the Giant Jamboree, which sums up and explains the project in less than 20 minutes:

Awards

  • Best Foundational Advance Project
  • Best New Basic Part

Nominations

  • Best Software
  • Best Hardware
  • Best New Composite Part
  • Best Part Collection


We established two orthogonal methods for the detection of unnatural base pairs in a target sequence: an Oxford Nanopore sequencing application and an enzyme based detection method



Development of a software suite for these orthogonal methods


Integration and characterization of the nucleotide transporter PtNTT2 from P.tricornutum in E.coli for the uptake of unnatural nucleoside triphosphates



Construction of a toolbox consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids



Colocalization of the RuBisCo and and subcellular compartment (carboxysome) using a fluorescent amino acid


Design, chemical synthesis and proof of functionality of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine



Modeling more than ten new aaRS sequences



Library development with several hundred thousand sequences for selecting aminoacyl-tRNA synthetases


Construction of positive and negative selection plasmids for the evolution of new synthetases for non-canonical amino acids



Improvement of an aminoacyl-tRNA synthetase test-system by introducing a FRET-system and development of a ranking system



Construction of an LED panel for irradiating 96-well microtiter plates, which can be used to manipulate non-canonical amino acids and for other applications



Development of an Android App to control the LED panel with your smartphone via Bluetooth


Writing a biosafety report entitled “Auxotrophy to Xeno-DNA: A Comprehensive Exploration of Combinatorial Mechanisms for a High-Fidelity Biosafety System”



Writing the ChImp Report on “Chances and Implications of an Expanded Genetic Code”