Difference between revisions of "Team:Bielefeld-CeBiTec"

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                                 var ranheight = "";
 
                                 var ranheight = "";
  
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                                 for(var k = 0; k < 1; k++){ranheight = ranheight + "" + rand(-100, 1700) + "" + ";";};
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                                 for(var k = 0; k < 1; k++){ranheight = ranheight + "" + ((rand(-100, 1800) + rand(-100, 1800) + rand(-100, 1800)) / 3) + "" + ";";};
  
 
                                 return ranheight;})
 
                                 return ranheight;})

Revision as of 18:38, 11 December 2017

Achievements


We established two orthogonal methods for the detection of unnatural base pairs in a target sequence: an Oxford Nanopore sequencing application and an enzyme based detection method



Development of a software suite for these orthogonal methods


Integration and characterization of the nucleotide transporter PtNTT2 from P.tricornutum in E.coli for the uptake of unnatural nucleoside triphosphates



Construction of a toolbox consisting of five aminoacyl-tRNA synthetases for incorporation of non-canonical amino acids



Colocalization of the RuBisCo and and subcellular compartment (carboxysome) using a fluorescent amino acid


Design, chemical synthesis and proof of functionality of a novel, fully synthetic amino acid based on cyanonitrobenzothiazol and asparagine



Modeling more than ten new aaRS sequences



Library development with several hundred thousand sequences for selecting aminoacyl-tRNA synthetases


Construction of positive and negative selection plasmids for the evolution of new synthetases for non-canonical amino acids



Improvement of an aminoacyl-tRNA synthetase test-system by introducing a FRET-system and development of a ranking system



Construction of an LED panel for irradiating 96-well microtiter plates, which can be used to manipulate non-canonical amino acids and for other applications



Development of an Android App to control the LED panel with your smartphone via Bluetooth


Writing a biosafety report entitled “Auxotrophy to Xeno-DNA: A Comprehensive Exploration of Combinatorial Mechanisms for a High-Fidelity Biosafety System”



Writing the ChImp Report on “Chances and Implications of an Expanded Genetic Code”