Difference between revisions of "Team:Cologne-Duesseldorf/Eric"

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</ul>
 
</ul>
 
<br>
 
<br>
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<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents  
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<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">
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 +
 
 +
<ul>
 +
<li>
 +
By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents  
 +
</li>
 +
</ul>
 +
 
 
<br>
 
<br>
−
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">With two different sensors we were able to efficiently  measure the pH and the redox potential inside our yeast peroxisomes.
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 +
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">
 +
 
 +
<ul>
 +
<li>
 +
With two different sensors we were able to efficiently  measure the pH and the redox potential inside our yeast peroxisomes.
 +
</li>
 +
</ul>
 +
 
 
<br>
 
<br>
−
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible.
+
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">
 +
 
 +
<ul>
 +
<li>
 +
Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible.
 +
 
 
<br>
 
<br>
−
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">By successfully translocating the required enzymes for the metabolic pathways of nootkatone and violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox
+
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">
 +
 
 +
<ul>
 +
<li>
 +
By successfully translocating the required enzymes for the metabolic pathways of nootkatone and violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox
 +
</li>
 +
</ul>
 +
 
 
<br>
 
<br>
−
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox.
+
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">
 +
 
 +
<ul>
 +
<li>
 +
We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox.
 +
</li>
 +
</ul>
 +
 
 
<br>
 
<br>
−
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">With a high throughput assay we characterized the import efficiency of different PTS2 sequences.
+
 
 +
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">
 +
 
 +
<ul>
 +
<li>
 +
With a high throughput assay we characterized the import efficiency of different PTS2 sequences.
 +
</li>
 +
</ul>
 +
 
 +
 
 
<br>
 
<br>
−
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment.
+
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">
 +
 
 +
<ul>
 +
<li>
 +
To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment.
 +
</li>
 +
</ul>
 +
 
 
<br>
 
<br>
−
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time.
+
 
 +
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">
 +
 
 +
<ul>
 +
<li>
 +
For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time.
 +
</li>
 +
</ul>
 +
 
 +
 
 
<br>
 
<br>
−
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">All our excellent results can be combined into a highly variable compartment toolbox for designing artificial compartments based on the peroxisomes in <i>S. cerevisiae</i> with an enormous range of applications.
+
<img src="https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg" style="max-width:100px;" align="left">
 +
 
 +
<ul>
 +
<li>
 +
All our excellent results can be combined into a highly variable compartment toolbox for designing artificial compartments based on the peroxisomes in <i>S. cerevisiae</i> with an enormous range of applications.
 +
</li>
 +
</ul>
 +
 
 
</p>
 
</p>
  

Revision as of 18:57, 1 November 2017




  • We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for the peroxisome in S. cerevisiae.

  • By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents

  • With two different sensors we were able to efficiently measure the pH and the redox potential inside our yeast peroxisomes.

  • Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible.
    • By successfully translocating the required enzymes for the metabolic pathways of nootkatone and violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox

    • We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox.

    • With a high throughput assay we characterized the import efficiency of different PTS2 sequences.

    • To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment.

    • For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time.

    • All our excellent results can be combined into a highly variable compartment toolbox for designing artificial compartments based on the peroxisomes in S. cerevisiae with an enormous range of applications.