Attributions We would like to sincerely thank everyone that has been involved with our project since its inception, contributing to its eventual completion. Throughout each step of the process we’ve been lucky to have had amazing support and guidance. Team members' contributions Human Practices Rosie Brady Julia Davis Iain Dunn Sam Garforth Andreas Hadjicharalambous Shu Ishida Alex Tep Harris Vince Wiki Gaurav Agarwal Rosie Brady Julia Davis Iain Dunn Sam Garforth Andreas Hadjicharalambous Shu Ishida Alex Tep Harris Vince Hannah Webb Eric LeGresley Modelling Iain Dunn Harris Vince Shu Ishida Parts Sam Garforth Collaboration Shu Ishida Rosie Brady Gaurav Agarwal Hannah Webb Julia Davis Iain Dunn Sam Garforth Alex Tep Harris Vince Delivery Method Rosie Brady Hannah Webb Harris Vince Sam Garforth Andreas Hadjicharalambous Characterisation Gaurav Agarwal Rosie Brady Julia Davis Sam Garforth Andreas Hadjicharalambous Eric LeGresley Poster Julia Davis Eric LeGresley Harris Vince Blog Rosie Brady Gaurav Agarwal Julia Davis Iain Dunn Sam Garforth Andreas Hadjicharalambous Shu Ishida Alex Tep Hannah Webb Cloning Gaurav Agarwal Julia Davis Rosie Brady Sam Garforth Andreas Hadjicharalambous Eric LeGresley Alex Tep Media Sam Garforth Harris Vince Hannah Webb Safety Gaurav Agarwal Rosie Brady Julia Davis Alex Tep Hannah Webb Special thanks to Dr George Wadhams Dr Wadhams has been central to the design and execution of the project from the very beginning, giving advice on wetlab and construct design, in addition to experimental approach. Dr Chris Jones Dr Jones has been hugely helpful to the students in the wet lab, teaching us how to use all the lab equipment and patiently answering our many day-to-day questions. His ability to explain protocols in an understanding way has allowed us to develop our lab skills considerably over the course of the summer. Professor Judy Armitage Professor Armitage generously allowed us to come into her lab for the summer, to complete the project under her supervision. Professor Anthony Watts Professor Watts has provided us with sponsorship support and has acted as our primary contact with the Biochemistry Department, specifically in relation to securing facilities and equipment. Professor Antonis Papachristodoulou Professor Papachristodoulou has provided us with guidance with respect to our modelling section. He has always been there to point us in the right direction and suggest new areas to explore. Thomas Folliard Tom has provided us with advice, particularly with respect to our plate reader experiments and cloning. His suggestions on how we might improve the efficiency of our cloning procedure were invaluable. He also provided us with a basic form of pCusC. Harrison Steel Harrison has provided crucial insights into understanding the data from plate reader experiments and also suggesting ways in which we might improve the our procedures. His past experience as an iGEM team member has also provided insights into how to get the most out of the competition. James Scott-Brown James has helped us develop our deterministic models and has been a key helper in trouble shooting our code. He also helped us analyse the data from the plate reader experiments. Also thanks to Dr Garry Brown Dr Brown gave the initial lecture that acted as the seed for our idea, and was happy to meet with us and discuss his experience with Wilson’s Disease. This guided the initial development of our project. Professor Kevin Foster Professor Foster provided us with valuable advice on our chassis and chelator choices. He also advised us on how we might make the bacteria persist for as long as possible in the small intestine. Professor Jane Kaye Professor Kaye acted as a springboard for the ethics and public engagement sections of our human practices, highlighting that policy is often dependent on public perception. Valerie Wheater and the Wilson’s Disease Support Group (WDSG) Valerie Wheater and the WDSG have been incredibly useful to our project, guiding us from the beginning with their thoughts on various aspects of our project. They invited us to present our project at their AGM and talk to many patients and doctors, which keenly illustrated to us the limitations of current treatments and the lack of rare disease awareness. Associate Professor Maike Bublitz Dr Bublitz gave us lots of very helpful advice on protein purification and other techniques we were unfamiliar with. Cardiff iGEM Our most sincere gratitude to all those at Cardiff iGEM in particular Rob Newman for setting up the collaboration, Dr. Geraint Parry for setting up the experiment and Dr. Anthony Hayes at the bioimaging unit measuring the our parts using the FLIM microscope. This took considerable time and expense for which we are very grateful. Crowd Funders and Sponsors Thank you to our kind Crowdfunders and sponsors for enabling us to have such a great summer! iGEM Thank you so much for this once-in-a-lifetime opportunity!