Difference between revisions of "Team:Georgia State/Collaborations"

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<h1 class="media-heading">Emory</h1>
 
<h1 class="media-heading">Emory</h1>
 
<p class="last">Emory, this year, had a cool project that focused on an issue faced by water refinery plants. Emory found during their visit to their local WaterHub that a primary concern was to come up with an efficient way to deal with constant fluctuating orthophosphate levels. Emory, to address this problem, decided to experiment on increasing the efficiency of organisms to eat phosphate. We assisted Emory in this endeavor by working with four strains of Bacillus subtilis. Two of the strains were wild-type isolated from water donated by the Emory Waterhub, and two of the strains were commercially available Bacillus subtilis. The goal of our work was to see which strain could uptake the most phosphate.  To test the bacteria we the protocol provided by Emory, see tab labeled Emory protocol. Then we modified the protocol, see modified protocol. And, finally, because we were lucky enough to visit the Georgia Aquarium a place with an abundant amount of water and a state of the art filtration system we asked the aquarium for some of their unfiltered and filtered water to see if they had some of the same phosphate problems.</p>
 
<p class="last">Emory, this year, had a cool project that focused on an issue faced by water refinery plants. Emory found during their visit to their local WaterHub that a primary concern was to come up with an efficient way to deal with constant fluctuating orthophosphate levels. Emory, to address this problem, decided to experiment on increasing the efficiency of organisms to eat phosphate. We assisted Emory in this endeavor by working with four strains of Bacillus subtilis. Two of the strains were wild-type isolated from water donated by the Emory Waterhub, and two of the strains were commercially available Bacillus subtilis. The goal of our work was to see which strain could uptake the most phosphate.  To test the bacteria we the protocol provided by Emory, see tab labeled Emory protocol. Then we modified the protocol, see modified protocol. And, finally, because we were lucky enough to visit the Georgia Aquarium a place with an abundant amount of water and a state of the art filtration system we asked the aquarium for some of their unfiltered and filtered water to see if they had some of the same phosphate problems.</p>
 
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                       <li class=""><a href="#Protocol" data-toggle="tab"> Emory Protocol</a></li>
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                       <li class=""><a href="#ASF" data-toggle="tab"> Atlanta Science Festival</a></li>
                       <li class=""><a href="#Hybrid" data-toggle="tab">GSU Emory Hybrid Protocol</a></li>
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                       <li class=""><a href="#STEM" data-toggle="tab">Accessibility In STEM</a></li>
 
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<li class=""><a href="#More" data-toggle="tab">SBC Speaker Series</a></li>
 
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                                 <p class="last">MA6b1. cells
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                                 <p class="last">We were requested to come back to ASF for 2017 due to how successful our Bio Brick activity was the previous year. We provided children and parents alike with a better understanding of the goals, applications and importance of synthetic biology by utilizing the bio brick activity and the glow fish display. This year, we educated many students and parents on synthetic biology and how to create a BioBrick construct using legos. The BioBrick activity utilizes colorful lego pieces to explain to attendees how to create a biobrick. This process involves selecting a plasmid base, promote sequence, ribosomal binding site and a coding sequence. This year used glow fish to relate how fluorescent proteins are utilized in real life. Glow fish are technicallyGMO’s that are appreciated in everyday life as a result of introducing the coding sequence from jellyfish and put it into the embryo of fish to produce a fluorescent glow. We also took some time to explained previous iGEM projects and their benefits to
 
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society. Lastly, we gave out a survey to get a better understanding of how many people are aware of the properties and benefits of CBD oil.</p>
<p class="last"> centrifuged 2500 rpm for 5 or 10 min depending on the sample</p>
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<p class="last">  LB supernatant was dumped</p>
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<p class="last"> cells resuspended in 30 mL of 1500 uM phosphate buffer</p>
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<p class="last">centrifuged at 2500 rpm for 10 min</p>
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<p class="last">phosphate buffer supernatant dumped</p>
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                                <img src="https://static.igem.org/mediawiki/2017/3/3d/T--Georgia_State--AtlantaSci_Poster.png" class="spacing-b no-spacing-l" alt="">
<p class="last"> cells resuspended in 32 mL of 1500 uM phosphate buffer</p>
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<p class="last"> 1 mL of resuspended cells put into 1.5 mL tubes labeled with the strain and time point</p>
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<p class="last">tubes centrifuged at 10k rpm for 5 mins at the specified time point</p>
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<p class="last">specified time points</p>
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        <p class="last">t0= 30 mins</p>
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        <p class="last">t2= 1 hr</p>
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        <p class="last">t3= 2.5 hrs</p>
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        <p class="last">t4= 24 hrs?</p>
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<p class="last">Leave overnight in a mixer at 37 C. </p>
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<p class="last">Next day, centrifuge 96 well plate.</p>
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<p class="last">Recipe for Malachite Green:</p>
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<p class="last">20 uL of the supernatant of each sample put into a new 96-well</p>
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<p class="last">60 uL of DI water added to each well with the sample to dilute</p>
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<p class="last">20 uL of Malachite green reagent added</p>
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<p class="last">Malachite Green - </p>
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<p class="last">500ul Malachite Green</p>
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<p class="last">125ul Ammonia </p>
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<p class="last">10ul Tween </p>
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                                 <p class="last">Over the summer the Georgia Center of the Death and Hard-of-Hearing (GCDHH) reached out to our GSU iGEM team to learn about synthetic biology and how they could be a part of the researching world. Without knowing how to approach the obstacle of communicating with one another, we reached out to our Department of Education to find out the best way to present our information to the incoming students. Putting the guidelines we received, we hosted a successful open lab day for the GCDHH students. We had interpreters signing as we were explaining the basics of our lab and the undergraduate research that iGEM has to offer. When we initially agreed to hosting the students, we had no idea how much it would impact our view of synthetic biology. We believe that the knowledge and experience we gain from being a part of iGEM should be accessible to everyone that has an interest. Furthermore, we have created our presentation to be more accessible and have gotten an opportunity to learn sign language. </p>  
 
                                 <p class="last">Over the summer the Georgia Center of the Death and Hard-of-Hearing (GCDHH) reached out to our GSU iGEM team to learn about synthetic biology and how they could be a part of the researching world. Without knowing how to approach the obstacle of communicating with one another, we reached out to our Department of Education to find out the best way to present our information to the incoming students. Putting the guidelines we received, we hosted a successful open lab day for the GCDHH students. We had interpreters signing as we were explaining the basics of our lab and the undergraduate research that iGEM has to offer. When we initially agreed to hosting the students, we had no idea how much it would impact our view of synthetic biology. We believe that the knowledge and experience we gain from being a part of iGEM should be accessible to everyone that has an interest. Furthermore, we have created our presentation to be more accessible and have gotten an opportunity to learn sign language. </p>  
 
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<div class="media-body"><img class="rotateimg90" src="https://static.igem.org/mediawiki/2017/b/b0/T--Georgia_State--STEMcamp5.jpg"  alt="">
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<div class="media-body"><img  class="rotateimg90" src="https://static.igem.org/mediawiki/2017/4/4c/T--Georgia_State--STEMcamp4.jpg" height="auto" width="auto" alt="">
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<h3> Salvation Army Boys & Girls Club</h3> 
 
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Revision as of 20:38, 1 November 2017

We were requested to come back to ASF for 2017 due to how successful our Bio Brick activity was the previous year. We provided children and parents alike with a better understanding of the goals, applications and importance of synthetic biology by utilizing the bio brick activity and the glow fish display. This year, we educated many students and parents on synthetic biology and how to create a BioBrick construct using legos. The BioBrick activity utilizes colorful lego pieces to explain to attendees how to create a biobrick. This process involves selecting a plasmid base, promote sequence, ribosomal binding site and a coding sequence. This year used glow fish to relate how fluorescent proteins are utilized in real life. Glow fish are technicallyGMO’s that are appreciated in everyday life as a result of introducing the coding sequence from jellyfish and put it into the embryo of fish to produce a fluorescent glow. We also took some time to explained previous iGEM projects and their benefits to society. Lastly, we gave out a survey to get a better understanding of how many people are aware of the properties and benefits of CBD oil.

GCDHH

Over the summer the Georgia Center of the Death and Hard-of-Hearing (GCDHH) reached out to our GSU iGEM team to learn about synthetic biology and how they could be a part of the researching world. Without knowing how to approach the obstacle of communicating with one another, we reached out to our Department of Education to find out the best way to present our information to the incoming students. Putting the guidelines we received, we hosted a successful open lab day for the GCDHH students. We had interpreters signing as we were explaining the basics of our lab and the undergraduate research that iGEM has to offer. When we initially agreed to hosting the students, we had no idea how much it would impact our view of synthetic biology. We believe that the knowledge and experience we gain from being a part of iGEM should be accessible to everyone that has an interest. Furthermore, we have created our presentation to be more accessible and have gotten an opportunity to learn sign language.



Salvation Army Boys & Girls Club

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