Difference between revisions of "Team:Georgia State/Collaborations"

 
(36 intermediate revisions by 3 users not shown)
Line 12: Line 12:
 
         <meta name="description" content="">
 
         <meta name="description" content="">
 
         <meta name="viewport" content="width=device-width">
 
         <meta name="viewport" content="width=device-width">
−
     
+
            <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Georgia_State/CSS?
−
      <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Georgia_State/CSS?
+
 
action=raw&ctype=text/css">
 
action=raw&ctype=text/css">
 
        
 
        
 +
 +
        <script type="text/javascript" src="https://2017.igem.org/Template:Georgia_State/JS?
 +
action=raw&ctype=text/javascript"></script>
 +
 
     </head>
 
     </head>
 
     <body>
 
     <body>
Line 27: Line 30:
 
                     <div class="logo">
 
                     <div class="logo">
 
                         <h1>Collaborations</h1>
 
                         <h1>Collaborations</h1>
−
                     </div>                
+
                     </div>
 +
 
 
<!-- Menu Start -->
 
<!-- Menu Start -->
 
                     <div class="menu">
 
                     <div class="menu">
Line 36: Line 40:
 
                             <div class="nav-collapse collapse">
 
                             <div class="nav-collapse collapse">
 
                                     <ul class="nav">
 
                                     <ul class="nav">
−
                                    <li><a href="https://2017.igem.org/Team:Georgia_State"  class="active">Home</a></li>
+
        <li><a href="https://2017.igem.org/Team:Georgia_State"  class="active">Home</a></li>
 +
        <li class="dropdown-submenu">
 +
            <a class="dropdown-toggle" data-toggle="dropdown">Team
 +
                <ul class="dropdown-menu">
 
                                     <li><a href="https://2017.igem.org/Team:Georgia_State/Team">Team</a></li>
 
                                     <li><a href="https://2017.igem.org/Team:Georgia_State/Team">Team</a></li>
−
                                     <li class="dropdown-submenu">
+
                                     <li><a href="https://2017.igem.org/Team:Georgia_State/Attributions">Attributions</a></li>
−
        <a href="https://2017.igem.org/Team:Georgia_State/Description" class="dropdown-toggle" data-toggle="dropdown">Project
+
                </ul>
−
                                          <ul class="dropdown-menu">
+
        </li>
−
                                          <li><a href="https://2017.igem.org/Team:Georgia_State/Results">Results</a></li>
+
 
−
                                          <li><a href="https://2017.igem.org/Team:Georgia_State/InterLab">Interlab</a></li>
+
        <li class="dropdown-submenu">
−
                            <li><a href="https://2017.igem.org/Team:Georgia_State/Collaborations">Collaborations</a></li>
+
            <a class="dropdown-toggle" data-toggle="dropdown">Project
−
                                          <li><a href="https://2017.igem.org/Team:Georgia_State/Attributions">Attributions</a></li>
+
                <ul class="dropdown-menu">
−
                                                                                                                    </ul>
+
                         
−
                                                                            </li>
+
                                <li><a href=" https://2017.igem.org/Team:Georgia_State/Description">Description</a></li>
−
                                    <li class="dropdown-submenu">
+
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Results">Results</a></li>                                        
−
                <a href="https://2017.igem.org/Team:Georgia_State/Parts" class="dropdown-toggle" data-toggle="dropdown">Parts
+
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Collaborations">Collaborations</a></li>
−
                                          <ul class="dropdown-menu">
+
                                                                                                                                                         
−
                                      <li><a href="https://2017.igem.org/Team:Georgia_State/Design">Design</a></li>
+
                </ul>
−
                                      <li><a href="https://2017.igem.org/Team:Georgia_State/Parts">Parts</a></li>
+
          </li>
−
                                      <li><a href="https://2017.igem.org/Team:Georgia_State/Contribution">Contribution</a></li>
+
       
−
                                          <li><a href="https://2017.igem.org/Team:Georgia_State/Improve">Improvement</a></li>
+
        <li class="dropdown-submenu">
−
                                                                                                        </ul>
+
            <a class="dropdown-toggle" data-toggle="dropdown">Parts
−
                                                                            </li>
+
                <ul class="dropdown-menu">
−
                                    <li><a href="https://2017.igem.org/Team:Georgia_State/Safety">Safety</a></li>
+
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Parts">Parts</a></li>
−
                                    <li><a href="https://2017.igem.org/Team:Georgia_State/Notebook">Notebook</a></li>
+
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Design">Design</a></li>    
−
                                    <li class="dropdown-submenu">
+
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Contribution">Contribution</a></li>
−
      <a href="https://2017.igem.org/Team:Georgia_State/Engagement" class="dropdown-toggle" data-toggle="dropdown">Human Practices
+
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Improve">Improvement</a></li>
−
                                          <ul class="dropdown-menu">
+
              </ul>
−
                            <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Silver">Silver</a></li>
+
          </li>
−
                        <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Gold_Integrated">Integrated and Gold</a></li>
+
        <li><a href="https://2017.igem.org/Team:Georgia_State/Safety">Safety</a></li>
−
                            <li><a href="https://2017.igem.org/Team:Georgia_State/Engagement">Public Engagement</a></li>
+
       
−
                                                                            </ul>
+
        <li class="dropdown-submenu">
−
                                                                            </li>
+
            <a class="dropdown-toggle" data-toggle="dropdown">Notebook
−
                            <li><a href="https://igem.org/2017_Judging_Form?team=Georgia_State">Judging</a></li>
+
                <ul class="dropdown-menu">
−
                                </ul>
+
                              <li><a href="https://2017.igem.org/Team:Georgia_State/Notebook">Notebook</a></li>  
−
                            </div>
+
                              <li><a href="https://2017.igem.org/Team:Georgia_State/Experiments">Experiments</a></li>
 +
                </ul>
 +
            </a>
 +
        </li>
 +
 
 +
        , <li class="dropdown-submenu">
 +
              <a class="dropdown-toggle" data-toggle="dropdown">Human Practices
 +
                <ul class="dropdown-menu">
 +
                          <li><a href="https://2017.igem.org/Team:Georgia_State/Engagement">Public Engagement</a></li>
 +
                          <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Silver">Silver</a></li>
 +
                          <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Gold_Integrated">Integrated and Gold</a>
 +
                              <ul><a href="https://2017.igem.org/Team:Georgia_State/HP/ASL">ASL Gallery</a></ul>
 +
                          </li>              
 +
                </ul>
 +
              </a>
 +
          </li>
 +
 
 +
          <li><a href="https://igem.org/2017_Judging_Form?team=Georgia_State">Judging</a></li>
 +
  </div>
 
<!--/.nav-collapse -->
 
<!--/.nav-collapse -->
 
                         </div>
 
                         </div>
Line 74: Line 99:
 
             <label>
 
             <label>
 
           <select class="selectnav"onChange="window.location.href=this.value">
 
           <select class="selectnav"onChange="window.location.href=this.value">
−
             <option value="https://2017.igem.org/Team:Georgia_State">Home</option>
+
             <option value="/Team:Georgia_State">Home</option>
−
             <option value="https://2017.igem.org/Team:Georgia_State">Team</option>
+
            <option value="/Team:Georgia_State/Team">Team</option>
−
             <option value="https://2017.igem.org/Team:Georgia_State">Project</option>
+
             <option value="/Team:Georgia_State/Attributions">Attributions</option>
−
             <option value="https://2017.igem.org/Team:Georgia_State">Parts</option>
+
            <option value="/Team:Georgia_State/Results">Results</option>
−
             <option value="https://2017.igem.org/Team:Georgia_State">Safety</option>
+
            <option value="/Team:Georgia_State/Collaborations">Collaborations</option>
−
             <option value="https://2017.igem.org/Team:Georgia_State">Human Practices</option>
+
             <option value="/Team:Georgia_State/Parts">Parts</option>
−
             <option value="https://2017.igem.org/Team:Georgia_State">Awards</option>
+
            <option value="/Team:Georgia_State/Description">Description</option>
−
             <option value="https://2017.igem.org/Team:Georgia_State">Notebook</option>
+
             <option value="/Team:Georgia_State/Design">Design</option>
 +
            <option value="/Team:Georgia_State/Contribution">Contribution</option>
 +
             <option value="/Team:Georgia_State/Improvement">Improve</option>
 +
            <option value="/Team:Georgia_State/Safety">Safety</option>
 +
             <option value="/Team:Georgia_State/Notebook">Notebook</option>
 +
            <option value="/Team:Georgia_State/Experiments">Experiments</option>
 +
             <option value="/Team:Georgia_State/Engagement">Public Engagement</option>
 +
            <option value="/Team:Georgia_State/HP/Silver">Silver</option>
 +
             <option value="/Team:Georgia_State/HP/Gold_Integrated">Gold and Integrated</option>
 +
            <option value="/Team:Georgia_State/HP/ASL">ASL Gallery</option>
 
             <option value="https://igem.org/2017_Judging_Form?team=Georgia_State">Judging</option>
 
             <option value="https://igem.org/2017_Judging_Form?team=Georgia_State">Judging</option>
 
           </select>
 
           </select>
Line 102: Line 136:
 
<div class="featured-blocks">
 
<div class="featured-blocks">
 
<div class="media-body">
 
<div class="media-body">
−
<h1 class="media-heading">The Plight of the Horseshoe Crab</h1>
+
<h1 class="media-heading">UVA Meet-Up</h1>
−
<p class="last"> To understand the plight of the horseshoe crab we decided to reach out to our local authorities, the Georgia Aquarium. The aquarium was able to provide an immersive learning environment. At the Georgia Aquarium, we were able to learn about the crab from our tour guide Max and able to touch the horseshoe crab, it was a tad bit slimy but cute nevertheless.  On our tour, we were made aware that horseshoe crabs are crucial to the food webs of many species and that with the depletion of the horseshoe crab population we see a reduction of a lot of other species, especially certain types of birds.  The crisis that the horseshoe crab faces was made clear by our tour guide, with the crabs serve as valuable resources for commercial fishers and the biomedical industry. But, in recent years concerns have been raised about the decline in Limuli population. Click on the tabs to learn about crab population threats and environmental impact of crab population depletion.</p>
+
<p class="last"> The University of Virginia iGEM team invited us along with William & Mary, University of Delaware, UNC-Asheville, and the University of Maryland to the Virginia Mid-Atlantic Meetup event. Each team gave a short presentation on their project. After every two presentations, there were break-out sessions. Most of the collaboration took place during the breakout sessions. Due to this opportunity, we were able to discuss and ask questions to the other teams about their projects.  The discussions helped us think about our project on an introspective level.  Through our conversations with William & Mary, we realized the importance of the rate of protein expression in a vector and its relation to our protein expression rates.  Analyzing how each group presented improved our overall presentation technique.
−
<h1 class="media-heading">The Plight of the Horseshoe Crab</h1>
+
After every presentation and break out session the Community Bio Labs from Charlottesville presented and then we had one final breakout session. This break-out session was the most important because we were able to speak directly with someone who had used HCG and pregnancy tests as a detection device. The conversation made us think about the concentration levels pregnancy tests detect and how we may need to alter our detection mechanism.</p>
−
<p class="last"> To understand the plight of the horseshoe crab we decided to reach out to our local authorities, the Georgia Aquarium. The aquarium was able to provide an immersive learning environment. At the Georgia Aquarium, we were able to learn about the crab from our tour guide Max and able to touch the horseshoe crab, it was a tad bit slimy but cute nevertheless.  On our tour, we were made aware that horseshoe crabs are crucial to the food webs of many species and that with the depletion of the horseshoe crab population we see a reduction of a lot of other species, especially certain types of birds. The crisis that the horseshoe crab faces was made clear by our tour guide, with the crabs serve as valuable resources for commercial fishers and the biomedical industry. But, in recent years concerns have been raised about the decline in Limuli population. Click on the tabs to learn about crab population threats and environmental impact of crab population depletion.</p>
+
</div>
 +
                         
 +
<div class="row-fluid">
 +
<div class="span4">
 +
<div class="media">
 +
<div class="media-body"><img src="https://static.igem.org/mediawiki/2017/8/8e/T--Georgia_State--UVAmeetup1.jpg"  alt="">
 +
</div>
 +
</div>
 +
</div>
 +
<div class="span4">
 +
<div class="media">
 +
<div class="media-body"><img src="https://static.igem.org/mediawiki/2017/7/7b/T--Georgia_State--UVAGROUP.jpeg" alt="">
 +
</div>
 +
</div>
 +
</div>
 +
<div class="span4">
 +
<div class="media">
 +
<div class="media-body"><img src="https://static.igem.org/mediawiki/2017/8/8b/T--Georgia_State--UVAmeetup2.jpg" height="auto" width="auto" alt="">
 +
</div>
 +
</div>
 +
</div>
  
 
</div>
 
</div>
 +
<div class="ruler-bottom"></div>
 +
<h1 class="media-heading">Emory</h1>
 +
<p class="last">Emory, this year, had a cool project that focused on an issue faced by water refinery plants. Emory found during their visit to their local WaterHub that a primary concern was to come up with an efficient way to deal with constant fluctuating orthophosphate levels. Emory, to address this problem, decided to experiment on increasing the efficiency of organisms to eat phosphate. We assisted Emory in this endeavor by working with four strains of Bacillus subtilis. Two of the strains were wild-type isolated from water donated by the Emory Waterhub, and two of the strains were commercially available Bacillus subtilis. The goal of our work was to see which strain could uptake the most phosphate.  To test the bacteria we the protocol provided by Emory, see tab labeled Emory protocol. Then we modified the protocol, see modified protocol. And, finally, because we were lucky enough to visit the Georgia Aquarium a place with an abundant amount of water and a state of the art filtration system we asked the aquarium for some of their unfiltered and filtered water to see if they had some of the same phosphate problems.</p>
 +
</div>
 +
            </div>
 +
            <div class="container">
 +
                <div class="tabs">
 +
                    <ul id="myTab" class="nav nav-tabs">
 +
                      <li class=""><a href="#Emory Protocol" data-toggle="tab"> Emory Protocol</a></li>
 +
                      <li class=""><a href="#Hybrid" data-toggle="tab">GSU- Emory Protocol</a></li>
  
 +
                                        </ul>
 +
                    <div id="myTabContent" class="tab-content">
 +
                      <div class="tab-pane fade active in" id="Emory Protocol">
 +
                        <div class="row-fluid">
 +
                            <div class="span6 ruler-right ruler-bottom">
 +
                                <div class="block">
 +
                                <p class="last">MA6b1. cells</p>
  
 +
<p class="last"> centrifuged 2500 rpm for 5 or 10 min depending on the sample</p>
 +
<p class="last">  LB supernatant was dumped</p>
 +
<p class="last"> cells resuspended in 30 mL of 1500 uM phosphate buffer</p>
 +
<p class="last">centrifuged at 2500 rpm for 10 min</p>
 +
<p class="last">phosphate buffer supernatant dumped</p>
 +
<p class="last"> cells resuspended in 32 mL of 1500 uM phosphate buffer</p>
 +
<p class="last"> 1 mL of resuspended cells put into 1.5 mL tubes labeled with the strain and time point</p>
 +
<p class="last">tubes centrifuged at 10k rpm for 5 mins at the specified time point</p>
 +
<p class="last">specified time points</p>
 +
        <p class="last">t0= 30 mins</p>
 +
        <p class="last">t2= 1 hr</p>
 +
        <p class="last">t3= 2.5 hrs</p>
 +
        <p class="last">t4= 24 hrs?</p>
  
−
</div>
+
<p class="last">Leave overnight in a mixer at 37 C. </p>
 +
<p class="last">Next day, centrifuge 96 well plate.</p>
  
 +
 +
<p class="last">Recipe for Malachite Green:</p>
 +
<p class="last">20 uL of the supernatant of each sample put into a new 96-well</p>
 +
<p class="last">60 uL of DI water added to each well with the sample to dilute</p>
 +
<p class="last">20 uL of Malachite green reagent added</p>
 +
 +
<p class="last">Malachite Green - </p>
 +
<p class="last">500ul Malachite Green</p>
 +
<p class="last">125ul Ammonia </p>
 +
<p class="last">10ul Tween </p>
 +
<br></br>
 +
 +
                                </div>
 +
                            </div>
 +
                            <div class="span6">
 +
                                <div class="block no-spacing-l">
 +
                                <img src="https://static.igem.org/mediawiki/2017/9/90/T--Georgia_State--Phosphate.png" class="spacing-b no-spacing-l" alt="">
 +
<h1 class="media-heading">Source</h1>
 +
<p class="last">Source for picture of phosphate model: https://2017.igem.org/File:T--Georgia_State--Phosphate.png</p>
 +
                                </div>
 +
                            </div>
 +
                        </div>
 +
                      </div>
 +
                      <div class="tab-pane fade" id="Hybrid">
 +
                        <div class="row-fluid">
 +
                            <div class="span10 ruler-bottom">
 +
                                <div class="block">
 +
                              <p class="last">MA6b1. cells</p>
 +
<p class="last"> Day One Program</p>
 +
<p class="last"> Take 5 mL of LB broth and place in 50 mL falcon tubes. Take a stab from glycerol stocks of two wild-types and two commercial strains(provided by Emory), place in tube. Let shake in the incubator overnight at 37 degrees.</p>
 +
<p class="last"> centrifuged 2500 rpm for 5 or 10 min depending on the sample</p>
 +
<p class="last">  LB supernatant was dumped</p>
 +
<p class="last"> cells resuspended in 30 mL of 1500 uM phosphate buffer</p>
 +
<p class="last">centrifuged at 2500 rpm for 10 min</p>
 +
<p class="last">phosphate buffer supernatant dumped</p>
 +
<p class="last"> cells resuspended in 32 mL of 1500 uM phosphate buffer</p>
 +
<p class="last"> 1 mL of resuspended cells put into 1.5 mL tubes labeled with the strain and time point</p>
 +
<p class="last">tubes centrifuged at 10k rpm for 5 mins at the specified time point</p>
 +
<p class="last">specified time points</p>
 +
        <p class="last">t0= 30 mins</p>
 +
        <p class="last">t2= 1 hr</p>
 +
        <p class="last">t3= 2.5 hrs</p>
 +
        <p class="last">t4= 5 hrs</p>
 +
        <p class="last">t4= 7.5 hrs</p>
 +
        <p class="last">t4= 24 hrs?</p>
 +
 +
<p class="last">Leave overnight in a mixer at 37 C. </p>
 +
<p class="last">Next day, centrifuge 96 well plate.</p>
 +
 +
 +
<p class="last">Recipe for Malachite Green:</p>
 +
<p class="last">20 uL of the supernatant of each sample put into a new 96-well</p>
 +
<p class="last">60 uL of DI water added to each well with the sample to dilute</p>
 +
<p class="last">20 uL of Malachite green reagent added</p>
 +
 +
 +
<br></br>
 +
 +
<br></br>
 +
</div>
 +
                         
 +
</div>
 
  </div>
 
  </div>
 
                         </div>
 
                         </div>
 
                           </div>
 
                           </div>
 +
<br><br><br>
 +
<a href="https://2017.igem.org/Team:Georgia_State"><h1 style="color:#ffffff; background-color:#A9CCE3; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: Trebuchet MS">Back to Home</h1> </a><br><br>
  
 
             <div class="container">
 
             <div class="container">

Latest revision as of 03:44, 2 November 2017

"It is the long history of humankind (and animal kind, too) those who learned to collaborate and improvise most effectively have prevailed."

Charles Darwin

UVA Meet-Up

The University of Virginia iGEM team invited us along with William & Mary, University of Delaware, UNC-Asheville, and the University of Maryland to the Virginia Mid-Atlantic Meetup event. Each team gave a short presentation on their project. After every two presentations, there were break-out sessions. Most of the collaboration took place during the breakout sessions. Due to this opportunity, we were able to discuss and ask questions to the other teams about their projects. The discussions helped us think about our project on an introspective level. Through our conversations with William & Mary, we realized the importance of the rate of protein expression in a vector and its relation to our protein expression rates. Analyzing how each group presented improved our overall presentation technique. After every presentation and break out session the Community Bio Labs from Charlottesville presented and then we had one final breakout session. This break-out session was the most important because we were able to speak directly with someone who had used HCG and pregnancy tests as a detection device. The conversation made us think about the concentration levels pregnancy tests detect and how we may need to alter our detection mechanism.

Emory

Emory, this year, had a cool project that focused on an issue faced by water refinery plants. Emory found during their visit to their local WaterHub that a primary concern was to come up with an efficient way to deal with constant fluctuating orthophosphate levels. Emory, to address this problem, decided to experiment on increasing the efficiency of organisms to eat phosphate. We assisted Emory in this endeavor by working with four strains of Bacillus subtilis. Two of the strains were wild-type isolated from water donated by the Emory Waterhub, and two of the strains were commercially available Bacillus subtilis. The goal of our work was to see which strain could uptake the most phosphate. To test the bacteria we the protocol provided by Emory, see tab labeled Emory protocol. Then we modified the protocol, see modified protocol. And, finally, because we were lucky enough to visit the Georgia Aquarium a place with an abundant amount of water and a state of the art filtration system we asked the aquarium for some of their unfiltered and filtered water to see if they had some of the same phosphate problems.

MA6b1. cells

centrifuged 2500 rpm for 5 or 10 min depending on the sample

LB supernatant was dumped

cells resuspended in 30 mL of 1500 uM phosphate buffer

centrifuged at 2500 rpm for 10 min

phosphate buffer supernatant dumped

cells resuspended in 32 mL of 1500 uM phosphate buffer

1 mL of resuspended cells put into 1.5 mL tubes labeled with the strain and time point

tubes centrifuged at 10k rpm for 5 mins at the specified time point

specified time points

t0= 30 mins

t2= 1 hr

t3= 2.5 hrs

t4= 24 hrs?

Leave overnight in a mixer at 37 C.

Next day, centrifuge 96 well plate.

Recipe for Malachite Green:

20 uL of the supernatant of each sample put into a new 96-well

60 uL of DI water added to each well with the sample to dilute

20 uL of Malachite green reagent added

Malachite Green -

500ul Malachite Green

125ul Ammonia

10ul Tween



Source

Source for picture of phosphate model: https://2017.igem.org/File:T--Georgia_State--Phosphate.png

MA6b1. cells

Day One Program

Take 5 mL of LB broth and place in 50 mL falcon tubes. Take a stab from glycerol stocks of two wild-types and two commercial strains(provided by Emory), place in tube. Let shake in the incubator overnight at 37 degrees.

centrifuged 2500 rpm for 5 or 10 min depending on the sample

LB supernatant was dumped

cells resuspended in 30 mL of 1500 uM phosphate buffer

centrifuged at 2500 rpm for 10 min

phosphate buffer supernatant dumped

cells resuspended in 32 mL of 1500 uM phosphate buffer

1 mL of resuspended cells put into 1.5 mL tubes labeled with the strain and time point

tubes centrifuged at 10k rpm for 5 mins at the specified time point

specified time points

t0= 30 mins

t2= 1 hr

t3= 2.5 hrs

t4= 5 hrs

t4= 7.5 hrs

t4= 24 hrs?

Leave overnight in a mixer at 37 C.

Next day, centrifuge 96 well plate.

Recipe for Malachite Green:

20 uL of the supernatant of each sample put into a new 96-well

60 uL of DI water added to each well with the sample to dilute

20 uL of Malachite green reagent added








Back to Home



Copyright(c) GSUiGEM / Original Design by:www.alltemplateneeds.com