Difference between revisions of "Team:Georgia State/Collaborations"

 
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                         <h1>Collaborations</h1>
 
                         <h1>Collaborations</h1>
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                                     <li><a href="https://2017.igem.org/Team:Georgia_State/Team">Team</a></li>
 
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                                     <li><a href="https://2017.igem.org/Team:Georgia_State/Attributions">Attributions</a></li>
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                                <li><a href=" https://2017.igem.org/Team:Georgia_State/Description">Description</a></li>
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                                <li><a href="https://2017.igem.org/Team:Georgia_State/Results">Results</a></li>                                        
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                                <li><a href="https://2017.igem.org/Team:Georgia_State/Collaborations">Collaborations</a></li>
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                                      <li><a href="https://2017.igem.org/Team:Georgia_State/Design">Design</a></li>
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                </ul>
                                      <li><a href="https://2017.igem.org/Team:Georgia_State/Parts">Parts</a></li>
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            <a class="dropdown-toggle" data-toggle="dropdown">Parts
                                                                            </li>
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                                    <li><a href="https://2017.igem.org/Team:Georgia_State/Safety">Safety</a></li>
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                                <li><a href="https://2017.igem.org/Team:Georgia_State/Parts">Parts</a></li>
                                    <li><a href="https://2017.igem.org/Team:Georgia_State/Notebook">Notebook</a></li>
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                                <li><a href="https://2017.igem.org/Team:Georgia_State/Design">Design</a></li>    
                                    <li class="dropdown-submenu">
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                                <li><a href="https://2017.igem.org/Team:Georgia_State/Contribution">Contribution</a></li>
      <a href="https://2017.igem.org/Team:Georgia_State/Engagement" class="dropdown-toggle" data-toggle="dropdown">Human Practices
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                                <li><a href="https://2017.igem.org/Team:Georgia_State/Improve">Improvement</a></li>
                                          <ul class="dropdown-menu">
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              </ul>
                            <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Silver">Silver</a></li>
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          </li>
                        <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Gold_Integrated">Integrated and Gold</a></li>
+
        <li><a href="https://2017.igem.org/Team:Georgia_State/Safety">Safety</a></li>
                            <li><a href="https://2017.igem.org/Team:Georgia_State/Engagement">Public Engagement</a></li>
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        <li class="dropdown-submenu">
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            <a class="dropdown-toggle" data-toggle="dropdown">Notebook
                            <li><a href="https://igem.org/2017_Judging_Form?team=Georgia_State">Judging</a></li>
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                              <li><a href="https://2017.igem.org/Team:Georgia_State/Notebook">Notebook</a></li>  
                            </div>
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                              <li><a href="https://2017.igem.org/Team:Georgia_State/Experiments">Experiments</a></li>
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        </li>
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 +
        , <li class="dropdown-submenu">
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              <a class="dropdown-toggle" data-toggle="dropdown">Human Practices
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                <ul class="dropdown-menu">
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                          <li><a href="https://2017.igem.org/Team:Georgia_State/Engagement">Public Engagement</a></li>
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                          <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Silver">Silver</a></li>
 +
                          <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Gold_Integrated">Integrated and Gold</a>
 +
                              <ul><a href="https://2017.igem.org/Team:Georgia_State/HP/ASL">ASL Gallery</a></ul>
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              </a>
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 +
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             <option value="/Team:Georgia_State">Home</option>
             <option value="https://2017.igem.org/Team:Georgia_State">Team</option>
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 +
            <option value="/Team:Georgia_State/HP/ASL">ASL Gallery</option>
 
             <option value="https://igem.org/2017_Judging_Form?team=Georgia_State">Judging</option>
 
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                     <ul id="myTab" class="nav nav-tabs">
                       <li class=""><a href="#ASF" data-toggle="tab"> Atlanta Science Festival</a></li>
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                       <li class=""><a href="#Emory Protocol" data-toggle="tab"> Emory Protocol</a></li>
                       <li class=""><a href="#STEM" data-toggle="tab">Accessibility In STEM</a></li>
+
                       <li class=""><a href="#Hybrid" data-toggle="tab">GSU- Emory Protocol</a></li>
<li class=""><a href="#More" data-toggle="tab">SBC Speaker Series</a></li>
+
 
 
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                                         </ul>
 
                     <div id="myTabContent" class="tab-content">
 
                     <div id="myTabContent" class="tab-content">
                       <div class="tab-pane fade active in" id="ASF">
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                       <div class="tab-pane fade active in" id="Emory Protocol">
 
                         <div class="row-fluid">
 
                         <div class="row-fluid">
 
                             <div class="span6 ruler-right ruler-bottom">
 
                             <div class="span6 ruler-right ruler-bottom">
 
                                 <div class="block">
 
                                 <div class="block">
                                 <p class="last">We were requested to come back to ASF for 2017 due to how successful our Bio Brick activity was the previous year. We provided children and parents alike with a better understanding of the goals, applications and importance of synthetic biology by utilizing the bio brick activity and the glow fish display. This year, we educated many students and parents on synthetic biology and how to create a BioBrick construct using legos. The BioBrick activity utilizes colorful lego pieces to explain to attendees how to create a biobrick. This process involves selecting a plasmid base, promote sequence, ribosomal binding site and a coding sequence. This year used glow fish to relate how fluorescent proteins are utilized in real life. Glow fish are technicallyGMO’s that are appreciated in everyday life as a result of introducing the coding sequence from jellyfish and put it into the embryo of fish to produce a fluorescent glow. We also took some time to explained previous iGEM projects and their benefits to
+
                                 <p class="last">MA6b1. cells</p>
society. Lastly, we gave out a survey to get a better understanding of how many people are aware of the properties and benefits of CBD oil.</p>
+
 
 +
<p class="last"> centrifuged 2500 rpm for 5 or 10 min depending on the sample</p>
 +
<p class="last">  LB supernatant was dumped</p>
 +
<p class="last"> cells resuspended in 30 mL of 1500 uM phosphate buffer</p>
 +
<p class="last">centrifuged at 2500 rpm for 10 min</p>
 +
<p class="last">phosphate buffer supernatant dumped</p>
 +
<p class="last"> cells resuspended in 32 mL of 1500 uM phosphate buffer</p>
 +
<p class="last"> 1 mL of resuspended cells put into 1.5 mL tubes labeled with the strain and time point</p>
 +
<p class="last">tubes centrifuged at 10k rpm for 5 mins at the specified time point</p>
 +
<p class="last">specified time points</p>
 +
        <p class="last">t0= 30 mins</p>
 +
        <p class="last">t2= 1 hr</p>
 +
        <p class="last">t3= 2.5 hrs</p>
 +
        <p class="last">t4= 24 hrs?</p>
 +
 
 +
<p class="last">Leave overnight in a mixer at 37 C. </p>
 +
<p class="last">Next day, centrifuge 96 well plate.</p>
 +
 
 +
 
 +
<p class="last">Recipe for Malachite Green:</p>
 +
<p class="last">20 uL of the supernatant of each sample put into a new 96-well</p>
 +
<p class="last">60 uL of DI water added to each well with the sample to dilute</p>
 +
<p class="last">20 uL of Malachite green reagent added</p>
 +
 
 +
<p class="last">Malachite Green - </p>
 +
<p class="last">500ul Malachite Green</p>
 +
<p class="last">125ul Ammonia </p>
 +
<p class="last">10ul Tween </p>
 +
<br></br>
 +
 
 
                                 </div>
 
                                 </div>
 
                             </div>
 
                             </div>
 
                             <div class="span6">
 
                             <div class="span6">
 
                                 <div class="block no-spacing-l">
 
                                 <div class="block no-spacing-l">
                                 <img src="https://static.igem.org/mediawiki/2017/3/3d/T--Georgia_State--AtlantaSci_Poster.png" class="spacing-b no-spacing-l" alt="">
+
                                 <img src="https://static.igem.org/mediawiki/2017/9/90/T--Georgia_State--Phosphate.png" class="spacing-b no-spacing-l" alt="">
 +
<h1 class="media-heading">Source</h1>
 +
<p class="last">Source for picture of phosphate model: https://2017.igem.org/File:T--Georgia_State--Phosphate.png</p>
 
                                 </div>
 
                                 </div>
 
                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
 
                       </div>
 
                       </div>
                       <div class="tab-pane fade" id="STEM">
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                       <div class="tab-pane fade" id="Hybrid">
 
                         <div class="row-fluid">
 
                         <div class="row-fluid">
 
                             <div class="span10 ruler-bottom">
 
                             <div class="span10 ruler-bottom">
 
                                 <div class="block">
 
                                 <div class="block">
<h3>GCDHH</h3>
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                              <p class="last">MA6b1. cells</p>
                                <p class="last">Over the summer the Georgia Center of the Death and Hard-of-Hearing (GCDHH) reached out to our GSU iGEM team to learn about synthetic biology and how they could be a part of the researching world. Without knowing how to approach the obstacle of communicating with one another, we reached out to our Department of Education to find out the best way to present our information to the incoming students. Putting the guidelines we received, we hosted a successful open lab day for the GCDHH students. We had interpreters signing as we were explaining the basics of our lab and the undergraduate research that iGEM has to offer. When we initially agreed to hosting the students, we had no idea how much it would impact our view of synthetic biology. We believe that the knowledge and experience we gain from being a part of iGEM should be accessible to everyone that has an interest. Furthermore, we have created our presentation to be more accessible and have gotten an opportunity to learn sign language. </p>  
+
<p class="last"> Day One Program</p>
 +
<p class="last"> Take 5 mL of LB broth and place in 50 mL falcon tubes. Take a stab from glycerol stocks of two wild-types and two commercial strains(provided by Emory), place in tube. Let shake in the incubator overnight at 37 degrees.</p>
 +
<p class="last"> centrifuged 2500 rpm for 5 or 10 min depending on the sample</p>
 +
<p class="last">  LB supernatant was dumped</p>
 +
<p class="last"> cells resuspended in 30 mL of 1500 uM phosphate buffer</p>
 +
<p class="last">centrifuged at 2500 rpm for 10 min</p>
 +
<p class="last">phosphate buffer supernatant dumped</p>
 +
<p class="last"> cells resuspended in 32 mL of 1500 uM phosphate buffer</p>
 +
<p class="last"> 1 mL of resuspended cells put into 1.5 mL tubes labeled with the strain and time point</p>
 +
<p class="last">tubes centrifuged at 10k rpm for 5 mins at the specified time point</p>
 +
<p class="last">specified time points</p>
 +
        <p class="last">t0= 30 mins</p>
 +
        <p class="last">t2= 1 hr</p>
 +
        <p class="last">t3= 2.5 hrs</p>
 +
        <p class="last">t4= 5 hrs</p>
 +
        <p class="last">t4= 7.5 hrs</p>
 +
        <p class="last">t4= 24 hrs?</p>
 +
 
 +
<p class="last">Leave overnight in a mixer at 37 C. </p>
 +
<p class="last">Next day, centrifuge 96 well plate.</p>
 +
 
 +
 
 +
<p class="last">Recipe for Malachite Green:</p>
 +
<p class="last">20 uL of the supernatant of each sample put into a new 96-well</p>
 +
<p class="last">60 uL of DI water added to each well with the sample to dilute</p>
 +
<p class="last">20 uL of Malachite green reagent added</p>
 +
 
 +
 
 +
<br></br>
 +
 
 
<br></br>
 
<br></br>
 
</div>
 
</div>
 
                            
 
                            
<div class="row-fluid">
 
<div class="span4">
 
<div class="media">
 
<div class="media-body"><img class="rotateimg90" src="https://static.igem.org/mediawiki/2017/b/b0/T--Georgia_State--STEMcamp5.jpg"  alt="">
 
</div>
 
</div>
 
</div>
 
<div class="span4">
 
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Latest revision as of 03:44, 2 November 2017

MA6b1. cells

centrifuged 2500 rpm for 5 or 10 min depending on the sample

LB supernatant was dumped

cells resuspended in 30 mL of 1500 uM phosphate buffer

centrifuged at 2500 rpm for 10 min

phosphate buffer supernatant dumped

cells resuspended in 32 mL of 1500 uM phosphate buffer

1 mL of resuspended cells put into 1.5 mL tubes labeled with the strain and time point

tubes centrifuged at 10k rpm for 5 mins at the specified time point

specified time points

t0= 30 mins

t2= 1 hr

t3= 2.5 hrs

t4= 24 hrs?

Leave overnight in a mixer at 37 C.

Next day, centrifuge 96 well plate.

Recipe for Malachite Green:

20 uL of the supernatant of each sample put into a new 96-well

60 uL of DI water added to each well with the sample to dilute

20 uL of Malachite green reagent added

Malachite Green -

500ul Malachite Green

125ul Ammonia

10ul Tween



Source

Source for picture of phosphate model: https://2017.igem.org/File:T--Georgia_State--Phosphate.png

MA6b1. cells

Day One Program

Take 5 mL of LB broth and place in 50 mL falcon tubes. Take a stab from glycerol stocks of two wild-types and two commercial strains(provided by Emory), place in tube. Let shake in the incubator overnight at 37 degrees.

centrifuged 2500 rpm for 5 or 10 min depending on the sample

LB supernatant was dumped

cells resuspended in 30 mL of 1500 uM phosphate buffer

centrifuged at 2500 rpm for 10 min

phosphate buffer supernatant dumped

cells resuspended in 32 mL of 1500 uM phosphate buffer

1 mL of resuspended cells put into 1.5 mL tubes labeled with the strain and time point

tubes centrifuged at 10k rpm for 5 mins at the specified time point

specified time points

t0= 30 mins

t2= 1 hr

t3= 2.5 hrs

t4= 5 hrs

t4= 7.5 hrs

t4= 24 hrs?

Leave overnight in a mixer at 37 C.

Next day, centrifuge 96 well plate.

Recipe for Malachite Green:

20 uL of the supernatant of each sample put into a new 96-well

60 uL of DI water added to each well with the sample to dilute

20 uL of Malachite green reagent added








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