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− | {{ | + | {{Georgia_State_sand}} |
<html> | <html> | ||
+ | <!DOCTYPE html> | ||
+ | <!--[if lt IE 7]> <html class="no-js lt-ie9 lt-ie8 lt-ie7"> <![endif]--> | ||
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+ | <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Georgia_State/CSS? | ||
+ | action=raw&ctype=text/css"> | ||
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+ | <p class="chromeframe">You are using an <strong>outdated</strong> browser. Please <a href="http://browsehappy.com/">upgrade your browser</a> or <a href="http://www.google.com/chromeframe/?redirect=true">activate Google Chrome Frame</a> to improve your experience.</p> | ||
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+ | <!-- This code is taken from http://twitter.github.com/bootstrap/examples/hero.html --> | ||
+ | <div class="container"> | ||
+ | <div class="site-header"> | ||
+ | <div class="logo"> | ||
+ | <h1>Improvement</h1> | ||
+ | </div> | ||
− | <div class=" | + | <!-- Menu Start --> |
− | < | + | <div class="menu"> |
− | < | + | <div class="navbar"> |
+ | <a class="btn btn-navbar" data-toggle="collapse" data-target=".nav-collapse"> | ||
+ | <i class="fw-icon-th-list"></i> | ||
+ | </a> | ||
+ | <div class="nav-collapse collapse"> | ||
+ | <ul class="nav"> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State" class="active">Home</a></li> | ||
+ | <li class="dropdown-submenu"> | ||
+ | <a class="dropdown-toggle" data-toggle="dropdown">Team | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/Team">Team</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/Attributions">Attributions</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
− | < | + | <li class="dropdown-submenu"> |
− | < | + | <a class="dropdown-toggle" data-toggle="dropdown">Project |
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/Description">Description</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/Design">Design</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/Results">Results</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/Collaborations">Collaborations</a></li> | ||
+ | |||
+ | </ul> | ||
+ | </li> | ||
+ | |||
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+ | <a class="dropdown-toggle" data-toggle="dropdown">Parts | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/Parts">Parts</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/PartsDesign">Design</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/Contribution">Contribution</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/Improve">Improvement</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/Safety">Safety</a></li> | ||
+ | |||
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+ | <a class="dropdown-toggle" data-toggle="dropdown">Notebook | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/Notebook">Notebook</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/Experiments">Experiments</a></li> | ||
+ | </ul> | ||
+ | </a> | ||
+ | </li> | ||
− | < | + | , <li class="dropdown-submenu"> |
− | < | + | <a class="dropdown-toggle" data-toggle="dropdown">Human Practices |
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/Engagement">Public Engagement</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Silver">Silver</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Gold_Integrated">Integrated and Gold</a> | ||
+ | <ul><a href="https://2017.igem.org/Team:Georgia_State/HP/ASL">ASL Gallery</a></ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </a> | ||
+ | </li> | ||
+ | |||
+ | <li><a href="https://igem.org/2017_Judging_Form?team=Georgia_State">Judging</a></li> | ||
+ | </div> | ||
+ | <!--/.nav-collapse --> | ||
+ | </div> | ||
+ | <div class="mini-menu"> | ||
+ | <label> | ||
+ | <select class="selectnav"onChange="window.location.href=this.value"> | ||
+ | <option value="/Team:Georgia_State">Home</option> | ||
+ | <option value="/Team:Georgia_State/Team">Team</option> | ||
+ | <option value="/Team:Georgia_State/Attributions">Attributions</option> | ||
+ | <option value="/Team:Georgia_State/Description">Description</option> | ||
+ | <option value="/Team:Georgia_State/Design">Design</option> | ||
+ | <option value="/Team:Georgia_State/Results">Results</option> | ||
+ | <option value="/Team:Georgia_State/Collaborations">Collaborations</option> | ||
+ | <option value="/Team:Georgia_State/Parts">Parts</option> | ||
+ | <option value="/Team:Georgia_State/PartsDesign">Parts Design</option> | ||
+ | <option value="/Team:Georgia_State/Contribution">Contribution</option> | ||
+ | <option value="/Team:Georgia_State/Improvement">Improve</option> | ||
+ | <option value="/Team:Georgia_State/Safety">Safety</option> | ||
+ | <option value="/Team:Georgia_State/Notebook">Notebook</option> | ||
+ | <option value="/Team:Georgia_State/Experiments">Experiments</option> | ||
+ | <option value="/Team:Georgia_State/Engagement">Public Engagement</option> | ||
+ | <option value="/Team:Georgia_State/HP/Silver">Silver</option> | ||
+ | <option value="/Team:Georgia_State/HP/Gold_Integrated">Gold and Integrated</option> | ||
+ | <option value="/Team:Georgia_State/HP/ASL">ASL Gallery</option> | ||
+ | <option value="https://igem.org/2017_Judging_Form?team=Georgia_State">Judging</option> | ||
+ | </select> | ||
+ | </label> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- Menu End --> | ||
− | </div> | + | <div class="container"> |
+ | <div class="featured-heading"> | ||
+ | <h1><i><font style="text-transform: none;">"Attempt the impossible in order to improve your work."</font></i></h2> | ||
+ | <h2>Bette Davis</h4> | ||
+ | </div> | ||
+ | <div class="column full_size"> | ||
+ | <h1>hCG</h1> | ||
+ | <p>In our search for a recombinant Human Chorionic Gonadotropin (hCG) beta subunit plasmid, we found that the part <a href="http://parts.igem.org/Part:BBa_K732001"> BBa_K732001 </a>from UVA was not codon optimized. Our attempt to express this part in E.coli failed. We have instead found the cDNA sequence of the beta-subunit from <a href="https://www.ncbi.nlm.nih.gov/pubmed/12477932">Strausberg, R.L. et al </a> and used it to improve the part. Thus, we were able to add the new improved part to the registry and this will allow for other teams to be able to work with a functional part with the optimized codon. Our new part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2524000"> BBa_K2524000 </a> could ideally be used to express hCG and be detected at low concentrations with a pregnancy test. </p> | ||
+ | |||
+ | </div> | ||
+ | <a href="https://2017.igem.org/Team:Georgia_State"><h1 style="color:#ffffff; background-color:#A9CCE3; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: Trebuchet MS">Back to Home</h1> </a><br><br> | ||
+ | <b>References</b><br> | ||
</html> | </html> |
Latest revision as of 03:18, 2 November 2017
Improvement
"Attempt the impossible in order to improve your work."
Bette Davis
hCG
In our search for a recombinant Human Chorionic Gonadotropin (hCG) beta subunit plasmid, we found that the part BBa_K732001 from UVA was not codon optimized. Our attempt to express this part in E.coli failed. We have instead found the cDNA sequence of the beta-subunit from Strausberg, R.L. et al and used it to improve the part. Thus, we were able to add the new improved part to the registry and this will allow for other teams to be able to work with a functional part with the optimized codon. Our new part BBa_K2524000 could ideally be used to express hCG and be detected at low concentrations with a pregnancy test.
Back to Home
References