Difference between revisions of "Team:Georgia State/Improve"

 
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                     <h1><i><font style="text-transform: none;">"Attempt the impossible in order to improve your work."</font></i></h2>
 
                     <h1><i><font style="text-transform: none;">"Attempt the impossible in order to improve your work."</font></i></h2>
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                     <h2>Robert Ingersoll</h4>
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                     <h2>Bette Davis</h4>
 
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<h1>Improve</h1>
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<h1>hCG</h1>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.</p>
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<p>In our search for a recombinant Human Chorionic Gonadotropin (hCG) beta subunit plasmid, we found that the part <a href="http://parts.igem.org/Part:BBa_K732001"> BBa_K732001 </a>from UVA was not codon optimized. Our attempt to express this part in E.coli failed. We have instead found the cDNA sequence of the beta-subunit from <a href="https://www.ncbi.nlm.nih.gov/pubmed/12477932">Strausberg, R.L. et al </a> and used it to improve the part. Thus, we were able to add the new improved part to the registry and this will allow for other teams to be able to work with a functional part with the optimized codon. Our new part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2524000"> BBa_K2524000 </a> could ideally be used to express hCG and be detected at low concentrations with a pregnancy test. </p>
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<h3>Gold Medal Criterion #2</h3>
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<p><b>Standard Tracks:</b> Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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<a href="https://2017.igem.org/Team:Georgia_State"><h1 style="color:#ffffff; background-color:#A9CCE3; -moz-border-radius: 15px; -webkit-border-radius: 15px; padding:15px; text-align: center; font-family: Trebuchet MS">Back to Home</h1> </a><br><br>
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<b>References</b><br>
  
 
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Latest revision as of 03:18, 2 November 2017

"Attempt the impossible in order to improve your work."

Bette Davis

hCG

In our search for a recombinant Human Chorionic Gonadotropin (hCG) beta subunit plasmid, we found that the part BBa_K732001 from UVA was not codon optimized. Our attempt to express this part in E.coli failed. We have instead found the cDNA sequence of the beta-subunit from Strausberg, R.L. et al and used it to improve the part. Thus, we were able to add the new improved part to the registry and this will allow for other teams to be able to work with a functional part with the optimized codon. Our new part BBa_K2524000 could ideally be used to express hCG and be detected at low concentrations with a pregnancy test.

Back to Home



References