iGEM IIT Delhi follows a high standard of safe and responsible biological engineering. We completely understand that we are responsible for living up to the trust placed in us to design, build, and share biological devices safely.
Lab access and rules (including appropriate clothing, eating and drinking, etc.).
Responsible individuals (such as lab or departmental specialist or institutional biosafety officer).
Differences between biosafety levels.
Biosafety equipment (such as biosafety cabinets).
Good microbial technique (such as lab practices).
Disinfection and sterilization.
Emergency procedures.
Chemicals, fire and electrical safety.
We didn’t have enough knowledge about the transport rules at first. Later through the collaboration on GMM Legislation we got a good insight of the transport rules in various parts of the world.
No work was conducted with Risk Group 3 or 4 organisms.
Chassis organism used : E.coli.
Strains of E.coli used : DH5-alpha, K-12, BL-21.
Description : Risk Group 1 organisms do not cause disease in healthy adult humans.
Lab Safety Level : Safety Level 1.
Besides our chassis, we used Streptomyces griseus (Orf2), Bacillus megaterium, Pseudomonas putida(SrpR) , Pseudomonas fluorescens (PhlF) and Bacillus subtilis (LmrA) because we are interested in repressor proteins expressed in these micro-organism. We have found the sequence and had it synthesized by a provider or fish it out from the organisms using PCR.
No research was conducted by the team in a Safety Level 3 or 4 laboratory.
The project is not associated with Risk Group 3 and 4 Organisms and therefore no work was conducted by the team in a Safety Level 3 or 4 laboratory.
iGEM IIT Delhi has always followed safe shipment guidelines while submitting samples. Certificates has always been processed and verified before any shipment. Here is ‘Non Infectious and Non Hazardous Certificate’ attached for shipment of one of the DNA samples.
iGEM IIT Delhi has not released or deployed our project outside of the laboratory at any time during the competition or at the Giant Jamboree.
iGEM IIT Delhi has always been following all biosafety rules of our institution and concerned biosafety laws of our country.
EHLSU (Environmental Health and Lab Safety Unit) is responsible for holding up all the safety guidelines in all the labs (including ours) at the institution. The guidelines can be found on the link -
http://dbtbiosafety.nic.in/guideline/pdf/guidelines_94.pdf
The safety guidelines for all the labs in our region (North India) are declared and monitored by EHLSU, whose rules can be found on the following link - http://dbtbiosafety.nic.in/guideline/pdf/guidelines_94.pdf
iGEM IIT Delhi did not conduct any experiment with human subjects (including non-invasive experiments, such as surveys), for the surveys conducted we have followed the rules of our institution.
iGEM IIT Delhi has always followed safe shipment guidelines while submitting samples. Certificates has always been processed and verified before any shipment. Here is ‘Non Infectious and Non Hazardous Certificate’ attached for shipment of one of the DNA samples.
iGEM IIT Delhi has not released or deployed our project outside of the laboratory at any time during the competition or at the Giant Jamboree.
