Difference between revisions of "Team:IIT Delhi/recipe"
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| − | 20x TAE buffer | + | <h2> 20x TAE buffer </h2> |
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<li>80mL of 1M Tris solution | <li>80mL of 1M Tris solution | ||
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| − | For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O. | + | <h2 id="pfont"> For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O. |
Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap | Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap | ||
the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and | the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and | ||
| − | store at room temperature. | + | store at room temperature. </h2> |
</div> | </div> | ||
</div> | </div> | ||
Revision as of 20:20, 31 October 2017
RECIPES
Accurate Experimenting for Accurate Results !!
20x TAE buffer
- 80mL of 1M Tris solution
- 0.7264g of EDTANa2 salt
- 2.284 mL glacial acetic acid
- distilled H2O 100mL
For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O. Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and store at room temperature.
- 50 mM glucose.
- 25 mM Tris-Cl (pH 8.0).
- 10 mM EDTA (pH 8.0).
0.2 N NaOH (freshly diluted from a 10 N stock).(100ulsds + 700ul mq + 200ul naoh)
1% (w/v) SDS.
- 5 M potassium acetate, 60.0 ml
- Glacial acetic acid, 11.5 ml
- H2O, 28.5 ml
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
