Team:IIT Delhi/recipe

iGEM IIT Delhi

RECIPES

                                                                                                                                                                                                                 

Accurate Experimenting for Accurate Results !!

20x TAE buffer
  • 80mL of 1M Tris solution
  • 0.7264g of EDTANa2 salt
  • 2.284 mL glacial acetic acid
  • distilled H2O 100mL
    For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O. Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and store at room temperature.
  • 50 mM glucose.
  • 25 mM Tris-Cl (pH 8.0).
  • 10 mM EDTA (pH 8.0).
    Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by autoclaving and store at 4°C.
    0.2 N NaOH (freshly diluted from a 10 N stock).(100ulsds + 700ul mq + 200ul naoh) 1% (w/v) SDS.
  • 5 M potassium acetate, 60.0 ml
  • Glacial acetic acid, 11.5 ml
  • H2O, 28.5 ml
    The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate. Store the solution at 4°C and transfer it to an ice bucket just before use.
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E-mail: iitd.igem@gmail.com
Undergraduate Laboratory
Department of Biotechnology and Biochemical Engineering, IIT Delhi